B4U609 (MURD_HYDS0) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase
EC=6.3.2.9

Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Gene names

Name:	murD
Ordered Locus Names:	HY04AAS1_1516

Organism

Hydrogenobaculum sp. (strain Y04AAS1) [Complete proteome] [HAMAP]

Taxonomic identifier

380749 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Hydrogenobaculum

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity HAMAP MF_00639.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_1000147408
Regions
	Nucleotide binding	92 – 98	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B4U609 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 55E29454C51F8759	FASTA	411	46,171
	10 20 30 40 50 60 MAYLVWGKGR SGLAAFNLLK AKGFKAYIGD DKEDKNLWQD VWNEIDTVVL SPGIPPFHPL 70 80 90 100 110 120 WKEAIKSSKE VIGETELAYR FYKGKNIIAV TGTDGKSTTV HLIHHFTSFK EGGNFGTPFS 130 140 150 160 170 180 EIVLDNDKEN VALEASSFQG KTLDTFRPNV GVFLNFSKDH LDWHPSLEDY LLSKQKIFKN 190 200 210 220 230 240 QTQEDILILN AQKPVCDTPS LAKKIFFGEN GDLKVVGSDV YYKDELLIEN ISHPSLKGLH 250 260 270 280 290 300 NLYNIAVASF VAFSMGISLE EIKKNLETFE ALPFRYQYLG NFEGIDICND SKSTTVNALM 310 320 330 340 350 360 SALESTKAPI LLIAGGIDKG GDFSSIEAYK DKIKAVFLYG KDKNLIKDQI EHFLKVYVLE 370 380 390 400 410 DLESALLKVK EQARKGDTIL FSPACASFDM FESYKHRGEV FNELVKKHFN S

References

[1]

"Complete and draft genome sequences of six members of the Aquificales." Reysenbach A.-L., Hamamura N., Podar M., Griffiths E., Ferreira S., Hochstein R., Heidelberg J., Johnson J., Mead D., Pohorille A., Sarmiento M., Schweighofer K., Seshadri R., Voytek M.A. J. Bacteriol. 191:1992-1993(2009) [PubMed: 19136599] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Y04AAS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001130 Genomic DNA. Translation: ACG58199.1.
RefSeq	YP_002122177.1. NC_011126.1.
3D structure databases
ProteinModelPortal	B4U609.
ModBase	Search...
Protein-protein interaction databases
STRING	B4U609.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6744347.
GenomeReviews	Gene locus HY04AAS1_1516 in contig CP001130_GR.
KEGG	hya:HY04AAS1_1516.
PATRIC	22137756. VBIHydSp64203_1522.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG750024.
OMA	ACASWDM.
ProtClustDB	CLSK2299927.
Family and domain databases
HAMAP	MF_00639. MurD. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01925.
PANTHER	PTHR23135:SF2. PTHR23135:SF2. 1 hit.
Pfam	PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01087. MurD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_HYDS0

Accession

Primary (citable) accession number: B4U609

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program