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B4U5Z5 (B4U5Z5_HYDS0) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172
EC=2.1.1.14 HAMAP MF_00172
Alternative name(s):
Cobalamin-independent methionine synthase HAMAP MF_00172
Methionine synthase, vitamin-B12 independent isozyme HAMAP MF_00172
Gene names
Name:metE HAMAP MF_00172
Ordered Locus Names:HY04AAS1_1502
OrganismHydrogenobaculum sp. (strain Y04AAS1) [Complete proteome] [HAMAP]
Taxonomic identifier380749 [NCBI]
Taxonomic lineageBacteria › Aquificae › Aquificales › Aquificaceae › Hydrogenobaculum

Protein attributes

Sequence length755 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172 SAAS SAAS013215

Catalytic activity

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172 SAAS SAAS013215

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172 SAAS SAAS013215

Sequence similarities

Belongs to the vitamin-B12 independent methionine synthase family. HAMAP MF_00172

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding6411Zinc By similarity HAMAP MF_00172
Metal binding6431Zinc By similarity HAMAP MF_00172
Metal binding7271Zinc By similarity HAMAP MF_00172

Sequences

Sequence LengthMass (Da)Tools
B4U5Z5 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: BA44DC8C4E121C5B

FASTA75587,551
        10         20         30         40         50         60 
MKALAYGYPK LGEKREFKNA LESFWKKAIT EEQFYEQMRN IEAQRIRAYS ESVDEIPVGE 

        70         80         90        100        110        120 
LSFYDFMLDT AVMVGMIPSR FGEYKGLETY FEMARGKSAM EMTKYFNTNY HYIVPELESN 

       130        140        150        160        170        180 
NFKLLENKPK RCYLNAKDKV QNPKPYLIAP FTFLKLSKTY KKTTKDGFSV LETSKIENEK 

       190        200        210        220        230        240 
TLYYFLEPLL EVYKDILKNL KTEGVNIVSL QDPGLVFDLK DYELNAVKKI YEELSQVSDI 

       250        260        270        280        290        300 
ELITYYDSVS AYKDIIDLPV KRIGLDLCSN AENLENLRKY GFPKDKELIA GIINGRQVWR 

       310        320        330        340        350        360 
ANLRDKLKLI DELSKIAPNL SISNSSPLFH LPVNVELETK MDKDLKDRLS FAKQKLEELK 

       370        380        390        400        410        420 
TLKNAFLGDK ESLKEVEASA KLFEGSFGKN QVVIDRIKAL KDSDFQRELP YKERIKLQQS 

       430        440        450        460        470        480 
ILNLPIFPTT TIGSFPQTEE VRKTRSAFRS GKISKQEYEA FIKSQIDNVI AFQEEIGLDV 

       490        500        510        520        530        540 
LVHGEFERTD MVEFFAEKLK GVATTEHGWI ISYGSRGYRP PIIYGDVYRD EPMTLNEILY 

       550        560        570        580        590        600 
AQSKTQKPVK GMLTGPVTIL NWSFYREDIP KKEVAYQIAL AILDEVKDLE KSGIKIIQID 

       610        620        630        640        650        660 
EPAFREGTPI KKKDWEDYFD WAIKSFRLSS KANPKTQIHT HMCYSEFNDI IDKIYDMDFD 

       670        680        690        700        710        720 
VISIEASRSK GEILEAFERF GKWDRQIGIG VYDIHSPTIP SVEEMEIVMK RAMKVLDKSL 

       730        740        750 
LWVNPDCGLK TRRWEEVKPA LKNMMEMALK LRKEA

« Hide

References

[1]"Complete and draft genome sequences of six members of the Aquificales."
Reysenbach A.-L., Hamamura N., Podar M., Griffiths E., Ferreira S., Hochstein R., Heidelberg J., Johnson J., Mead D., Pohorille A., Sarmiento M., Schweighofer K., Seshadri R., Voytek M.A.
J. Bacteriol. 191:1992-1993(2009) [PubMed: 19136599] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001130 Genomic DNA. Translation: ACG58185.1.
RefSeqYP_002122163.1. NC_011126.1.

3D structure databases

ProteinModelPortalB4U5Z5.
SMRB4U5Z5. Positions 2-754.
ModBaseSearch...

Protein-protein interaction databases

STRINGB4U5Z5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6744333.
GenomeReviewsGene locus HY04AAS1_1502 in contig CP001130_GR.
KEGGhya:HY04AAS1_1502.
PATRIC22137728. VBIHydSp64203_1508.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG287495.
OMAMLDTAVM.
ProtClustDBPRK05222.

Family and domain databases

HAMAPMF_00172. Meth_synth.
[Tree]
InterProIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Methionine_synth.
[Graphical view]
KOK00549.
PfamPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsTIGR01371. Met_syn_B12ind. 1 hit.
ProtoNetSearch...

Entry information

Entry nameB4U5Z5_HYDS0
AccessionPrimary (citable) accession number: B4U5Z5
Entry history
Integrated into UniProtKB/TrEMBL: September 23, 2008
Last sequence update: September 23, 2008
Last modified: December 14, 2011
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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