B4U5Z5 (B4U5Z5_HYDS0) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP-Rule MF_00172
EC=2.1.1.14 HAMAP-Rule MF_00172

Alternative name(s):
Cobalamin-independent methionine synthase HAMAP-Rule MF_00172
Methionine synthase, vitamin-B12 independent isozyme HAMAP-Rule MF_00172

Gene names

Name:	metE HAMAP-Rule MF_00172
Ordered Locus Names:	HY04AAS1_1502

Organism

Hydrogenobaculum sp. (strain Y04AAS1) [Complete proteome] [HAMAP] EMBL ACG58185.1

Taxonomic identifier

380749 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Aquificae › Aquificae › Aquificales › Aquificaceae › Hydrogenobaculum

Protein attributes

Sequence length	755 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP-Rule MF_00172 SAAS SAAS013215
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP-Rule MF_00172 SAAS SAAS013215
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP-Rule MF_00172 SAAS SAAS013215
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family. HAMAP-Rule MF_00172

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis HAMAP-Rule MF_00172 SAAS SAAS013215
Domain	Repeat HAMAP-Rule MF_00172
Ligand	Metal-binding HAMAP-Rule MF_00172 SAAS SAAS013215 Zinc HAMAP-Rule MF_00172 SAAS SAAS013215
Molecular function	Methyltransferase HAMAP-Rule MF_00172 SAAS SAAS013215 EMBL ACG58185.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	641	1	Zinc By similarity HAMAP-Rule MF_00172
	Metal binding	643	1	Zinc By similarity HAMAP-Rule MF_00172
	Metal binding	727	1	Zinc By similarity HAMAP-Rule MF_00172

Sequences

	Sequence		Length	Mass (Da)
	B4U5Z5 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: BA44DC8C4E121C5B	FASTA	755	87,551
	10 20 30 40 50 60 MKALAYGYPK LGEKREFKNA LESFWKKAIT EEQFYEQMRN IEAQRIRAYS ESVDEIPVGE 70 80 90 100 110 120 LSFYDFMLDT AVMVGMIPSR FGEYKGLETY FEMARGKSAM EMTKYFNTNY HYIVPELESN 130 140 150 160 170 180 NFKLLENKPK RCYLNAKDKV QNPKPYLIAP FTFLKLSKTY KKTTKDGFSV LETSKIENEK 190 200 210 220 230 240 TLYYFLEPLL EVYKDILKNL KTEGVNIVSL QDPGLVFDLK DYELNAVKKI YEELSQVSDI 250 260 270 280 290 300 ELITYYDSVS AYKDIIDLPV KRIGLDLCSN AENLENLRKY GFPKDKELIA GIINGRQVWR 310 320 330 340 350 360 ANLRDKLKLI DELSKIAPNL SISNSSPLFH LPVNVELETK MDKDLKDRLS FAKQKLEELK 370 380 390 400 410 420 TLKNAFLGDK ESLKEVEASA KLFEGSFGKN QVVIDRIKAL KDSDFQRELP YKERIKLQQS 430 440 450 460 470 480 ILNLPIFPTT TIGSFPQTEE VRKTRSAFRS GKISKQEYEA FIKSQIDNVI AFQEEIGLDV 490 500 510 520 530 540 LVHGEFERTD MVEFFAEKLK GVATTEHGWI ISYGSRGYRP PIIYGDVYRD EPMTLNEILY 550 560 570 580 590 600 AQSKTQKPVK GMLTGPVTIL NWSFYREDIP KKEVAYQIAL AILDEVKDLE KSGIKIIQID 610 620 630 640 650 660 EPAFREGTPI KKKDWEDYFD WAIKSFRLSS KANPKTQIHT HMCYSEFNDI IDKIYDMDFD 670 680 690 700 710 720 VISIEASRSK GEILEAFERF GKWDRQIGIG VYDIHSPTIP SVEEMEIVMK RAMKVLDKSL 730 740 750 LWVNPDCGLK TRRWEEVKPA LKNMMEMALK LRKEA

References

[1]

"Complete and draft genome sequences of six members of the Aquificales." Reysenbach A.L., Hamamura N., Podar M., Griffiths E., Ferreira S., Hochstein R., Heidelberg J., Johnson J., Mead D., Pohorille A., Sarmiento M., Schweighofer K., Seshadri R., Voytek M.A. J. Bacteriol. 191:1992-1993(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Y04AAS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001130 Genomic DNA. Translation: ACG58185.1.
RefSeq	YP_002122163.1. NC_011126.1.
3D structure databases
ProteinModelPortal	B4U5Z5.
SMR	B4U5Z5. Positions 2-754.
ModBase	Search...
Protein-protein interaction databases
STRING	380749.HY04AAS1_1502.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACG58185; ACG58185; HY04AAS1_1502.
GeneID	6744333.
KEGG	hya:HY04AAS1_1502.
PATRIC	22137728. VBIHydSp64203_1508.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0620.
HOGENOM	HOG000246221.
KO	K00549.
OMA	QEFSIAY.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	HSP380749:GH30-1555-MONOMER.
UniPathway	UPA00051; UER00082.
Family and domain databases
HAMAP	MF_00172. Meth_synth.
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B4U5Z5_HYDS0

Accession

Primary (citable) accession number: B4U5Z5

Entry history

Integrated into UniProtKB/TrEMBL:	September 23, 2008
Last sequence update:	September 23, 2008
Last modified:	May 1, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)