B4TQQ5 (LEXA_SALSV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
LexA repressor
EC=3.4.21.88

Gene names

Name:	lexA
Ordered Locus Names:	SeSA_A4429

Organism

Salmonella schwarzengrund (strain CVM19633) [Complete proteome] [HAMAP]

Taxonomic identifier

439843 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella enterica subsp. enterica serovar Schwarzengrund

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity. HAMAP-Rule MF_00015
Catalytic activity	Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP-Rule MF_00015
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the peptidase S24 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Hydrolase Repressor
PTM	Autocatalytic cleavage
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP proteolysis Inferred from electronic annotation. Source: InterPro transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: HAMAP serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 202	202	LexA repressor HAMAP-Rule MF_00015		PRO_1000089597
Regions
	DNA binding	28 – 48	21	H-T-H motif By similarity
Sites
	Active site	119	1	For autocatalytic cleavage activity By similarity
	Active site	156	1	For autocatalytic cleavage activity By similarity
	Site	84 – 85	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4TQQ5 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 6BC34EA11A70430C	FASTA	202	22,305
	10 20 30 40 50 60 MKALTARQQE VFDLIRDHIS QTGMPPTRAE IAQRLGFRSP NAAEEHLKAL ARKGVLEIVS 70 80 90 100 110 120 GASRGIRLLQ EEEDGLPLVG RVAAGEPLLA QQHIEGHYQV DPSLFKPSAD FLLRVSGMSM 130 140 150 160 170 180 KDIGIMDGDL LAVHKTQDVR NGQVVVARID DEVTVKRLKK QGNKVELLPE NSEFTPIVVD 190 200 LREQSFTIEG LAVGVIRNGE WL

References

[1]

"Complete genome of Salmonella schwarzengrund strain CVM19633." Ravel J., Fricke W.F., White D., McDermott P., Mammel M., Rosovitz M., Leclerc J., Cebula T., Sebastian Y. Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CVM19633.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001127 Genomic DNA. Translation: ACF93006.1.
RefSeq	YP_002117116.1. NC_011094.1.
3D structure databases
ProteinModelPortal	B4TQQ5.
SMR	B4TQQ5. Positions 2-198.
ModBase	Search...
Protein-protein interaction databases
STRING	439843.SeSA_A4429.
Protein family/group databases
MEROPS	S24.001.
Proteomic databases
PRIDE	B4TQQ5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACF93006; ACF93006; SeSA_A4429.
GeneID	6519254.
KEGG	sew:SeSA_A4429.
PATRIC	32377419. VBISalEnt87589_4389.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1974.
HOGENOM	HOG000232167.
KO	K01356.
OMA	RGDSMKN.
ProtClustDB	PRK00215.
Enzyme and pathway databases
BioCyc	SENT439843:GHHR-4529-MONOMER.
Family and domain databases
Gene3D	1.10.10.10. 1 hit. 2.10.109.10. 1 hit.
HAMAP	MF_00015. LexA.
InterPro	IPR006199. LexA_DNA-bd_dom. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA. IPR019759. Peptidase_S24_S26. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00726. LEXASERPTASE.
SUPFAM	SSF51306. Pept_S24_S26_C. 1 hit.
TIGRFAMs	TIGR00498. lexA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEXA_SALSV

Accession

Primary (citable) accession number: B4TQQ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	May 1, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program