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Protein

Phosphomethylpyrimidine synthase

Gene

thiC

Organism
Salmonella schwarzengrund (strain CVM19633)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.UniRule annotation

Catalytic activityi

5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine = 4-amino-2-methyl-5-(phosphomethyl)pyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei239SubstrateUniRule annotation1
Binding sitei268SubstrateUniRule annotation1
Binding sitei297SubstrateUniRule annotation1
Binding sitei333SubstrateUniRule annotation1
Binding sitei433SubstrateUniRule annotation1
Metal bindingi437ZincUniRule annotation1
Binding sitei460SubstrateUniRule annotation1
Metal bindingi501ZincUniRule annotation1
Metal bindingi581Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi584Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi589Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processThiamine biosynthesis
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine, Zinc

Enzyme and pathway databases

UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphomethylpyrimidine synthaseUniRule annotation (EC:4.1.99.17UniRule annotation)
Alternative name(s):
Hydroxymethylpyrimidine phosphate synthaseUniRule annotation
Short name:
HMP-P synthaseUniRule annotation
Short name:
HMP-phosphate synthaseUniRule annotation
Short name:
HMPP synthaseUniRule annotation
Thiamine biosynthesis protein ThiCUniRule annotation
Gene namesi
Name:thiCUniRule annotation
Ordered Locus Names:SeSA_A4374
OrganismiSalmonella schwarzengrund (strain CVM19633)
Taxonomic identifieri439843 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001865 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000932341 – 631Phosphomethylpyrimidine synthaseAdd BLAST631

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB4TQK4
SMRiB4TQK4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni353 – 355Substrate bindingUniRule annotation3
Regioni394 – 397Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the ThiC family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000224484
KOiK03147
OMAiTWELFRD

Family and domain databases

Gene3Di3.20.20.540, 1 hit
HAMAPiMF_00089 ThiC, 1 hit
InterProiView protein in InterPro
IPR037509 ThiC
IPR025747 ThiC-associated_dom
IPR038521 ThiC/Bza_sf
IPR002817 ThiC/BzaA/B
PANTHERiPTHR30557 PTHR30557, 1 hit
PfamiView protein in Pfam
PF13667 ThiC-associated, 1 hit
PF01964 ThiC_Rad_SAM, 1 hit
SFLDiSFLDF00407 phosphomethylpyrimidine_syntha, 1 hit
TIGRFAMsiTIGR00190 thiC, 1 hit

Sequencei

Sequence statusi: Complete.

B4TQK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTTTLTRRE QRAKAQHFID TLEGTAFPNS KRIYVTGSQH DIRVPMREIQ
60 70 80 90 100
LSPTLIGGSK DNPQFEENEA VPVYDTSGPY GDPEVAINVQ QGLAKLRQPW
110 120 130 140 150
IDARNDSEEL DDRSSAYTRE RLADDGLDDL RFTGLLTPKR AKAGKRVTQL
160 170 180 190 200
HYARNGIVTP EMEFIAIREN MGRERIRSEV LRHQHPGMSF GARLPENITP
210 220 230 240 250
EFVRDEVAAG RAIIPANINH PESEPMIIGR NFLVKVNANI GNSAVTSSIE
260 270 280 290 300
EEVEKLVWAT RWGADTVMDL STGRYIHETR EWILRNSPVP IGTVPIYQAL
310 320 330 340 350
EKVNGIAEDL TWEAFRDTLL EQAEQGVDYF TIHAGVLLRY VPMTAKRLTG
360 370 380 390 400
IVSRGGSIMA KWCLSHHKEN FLFEHFREIC EICAAYDVSL SLGDGLRPGS
410 420 430 440 450
IQDANDEAQF SELHTLGELT KIAWEYDVQV MIEGPGHVPM HMIQRNMTEE
460 470 480 490 500
LEHCHEAPFY TLGPLTTDIA PGYDHFTSGI GAAMIGWFGC AMLCYVTPKE
510 520 530 540 550
HLGLPNKEDV KQGLITYKIA AHAADLAKGH PGAQIRDNAM SKARFEFRWE
560 570 580 590 600
DQFNLALDPF TARAWHDETL PQESGKVAHF CSMCGPKFCS MKISQEVRDY
610 620 630
AAAQTIEVGM ADMSENFRAK GGEIYLKREE V
Length:631
Mass (Da):70,874
Last modified:September 23, 2008 - v1
Checksum:i682A964C7A468E8D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001127 Genomic DNA Translation: ACF89304.1
RefSeqiWP_000108416.1, NC_011094.1

Genome annotation databases

EnsemblBacteriaiACF89304; ACF89304; SeSA_A4374
KEGGisew:SeSA_A4374

Similar proteinsi

Entry informationi

Entry nameiTHIC_SALSV
AccessioniPrimary (citable) accession number: B4TQK4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: March 28, 2018
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health