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B4TPU7 (GLPK_SALSV) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycerol kinase

EC=2.7.1.30
Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK
Gene names
Name:glpK
Ordered Locus Names:SeSA_A4302
OrganismSalmonella schwarzengrund (strain CVM19633) [Complete proteome] [HAMAP]
Taxonomic identifier439843 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length502 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate By similarity. HAMAP-Rule MF_00186

Catalytic activity

ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP-Rule MF_00186

Enzyme regulation

Activity of this regulatory enzyme is affected by several metabolites. Allosterically and non-competitively inhibited by fructose 1,6-bisphosphate (FBP) and unphosphorylated phosphocarrier protein EIIA-Glc (III-Glc), an integral component of the bacterial phosphotransferase (PTS) system By similarity. HAMAP-Rule MF_00186

Pathway

Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP-Rule MF_00186

Subunit structure

Homotetramer and homodimer (in equilibrium). Heterodimer with EIIA-Glc. Binds 1 zinc ion per glycerol kinase EIIA-Glc dimer. The zinc ion is important for dimerization By similarity.

Sequence similarities

Belongs to the FGGY kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 502502Glycerol kinase HAMAP-Rule MF_00186
PRO_1000098761

Regions

Nucleotide binding14 – 163ATP By similarity
Nucleotide binding412 – 4165ATP By similarity
Region84 – 852Substrate binding By similarity
Region234 – 2363Allosteric FBP inhibitor binding HAMAP-Rule MF_00186
Region246 – 2472Substrate binding By similarity

Sites

Metal binding4791Zinc; shared with EIIA-Glc By similarity
Binding site141Substrate By similarity
Binding site181ATP By similarity
Binding site1361Substrate By similarity
Binding site2681ATP By similarity
Binding site3111ATP; via carbonyl oxygen By similarity
Binding site3151ATP; via amide nitrogen By similarity
Binding site3301ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B4TPU7 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: A780A522CA16C1B4

FASTA50256,052
        10         20         30         40         50         60 
MTEKKYIVAL DQGTTSSRAV VMDHDANIVS VSQREFEQIY PKPGWVEHDP MEIWASQSST 

        70         80         90        100        110        120 
LVEVLAKADI SSDQIAAIGI TNQRETAIVW ERETGKPIYN AIVWQCRRTA DICEQLKRDG 

       130        140        150        160        170        180 
LEDYIRDNTG LVVDPYFSGT KVKWILDHVE GSRERAKRGE LLFGTVDTWL IWKMTQGRVH 

       190        200        210        220        230        240 
VTDYTNASRT MLFNIHDLDW DDKMLDVLDI PRAMLPQVRK SSEVYGQTNI GGKGGTRIPI 

       250        260        270        280        290        300 
AGIAGDQQAA LFGQLCVKEG MAKNTYGTGC FMLMNTGEKA VKSENGLLTT IACGPSGEVN 

       310        320        330        340        350        360 
YALEGAVFMA GASIQWLRDE MKLISDAFDS EYFATKVKDT NGVYVVPAFT GLGAPYWDPY 

       370        380        390        400        410        420 
ARGAIFGLTR GVNSNHIIRA TLESIAYQTR DVLEAMQADS GIRLHALRVD GGAVANNFLM 

       430        440        450        460        470        480 
QFQSDILGTR VERPEVREVT ALGAAYLAGL AVGYWQNLDE LQEKAVIERE FRPGIETTER 

       490        500 
NYRYSGWKKA VKRAMAWEEH DK 

« Hide

References

[1]"Complete genome of Salmonella schwarzengrund strain CVM19633."
Ravel J., Fricke W.F., White D., McDermott P., Mammel M., Rosovitz M., Leclerc J., Cebula T., Sebastian Y.
Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CVM19633.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001127 Genomic DNA. Translation: ACF90443.1.
RefSeqYP_002117007.1. NC_011094.1.

3D structure databases

ProteinModelPortalB4TPU7.
SMRB4TPU7. Positions 3-501.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING439843.SeSA_A4302.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACF90443; ACF90443; SeSA_A4302.
PATRIC32377167. VBISalEnt87589_4275.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0554.
HOGENOMHOG000222134.
OMAALYGQLC.
OrthoDBEOG6RZB46.
ProtClustDBPRK00047.

Enzyme and pathway databases

BioCycSENT439843:GHHR-1874-MONOMER.
UniPathwayUPA00618; UER00672.

Family and domain databases

HAMAPMF_00186. Glycerol_kin.
InterProIPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01311. glycerol_kin. 1 hit.
PROSITEPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLPK_SALSV
AccessionPrimary (citable) accession number: B4TPU7
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: February 19, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways