Reviewed, UniProtKB/Swiss-Prot B4TKU5 (RTCA_SALHS)

Names and origin

Protein names

Recommended name:
    Probable RNA 3'-terminal phosphate cyclase
      Short name=RNA-3'-phosphate cyclase
      Short name=RNA cyclase
    EC=6.5.1.4

Gene names

Name:	rtcA
Ordered Locus Names:	SeHA_C3826

Organism

Salmonella heidelberg (strain SL476) [Complete proteome] [HAMAP]

Taxonomic identifier

454169 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella enterica subsp. enterica serovar Heidelberg

Protein attributes

Sequence length	353 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity.
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 353	353	Probable RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_1000099354
Sites
	Active site	322	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4TKU5-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 7FBFCE2783230F6C	FASTA	353	37,050
	10 20 30 40 50 60 MARIIALDGA QGEGGGQILR SALSLSMITG QPFEMSGIRA GRAKPGLLRQ HLTAVRAATE 70 80 90 100 110 120 ICGAQVNGDE LGSQQLRFTP GPIRGGEYRF AIGSAGSCML VLQTVLPALW FADGSSRVEV 130 140 150 160 170 180 HGGTHNQAAP SADFICRVWE PLLARMGISQ RTTLIKHGFY PAGGGAAATV VEPATSLRGL 190 200 210 220 230 240 TLISRGETLR TTAEALLAAV PYHVGEREVA TLEAHFPQAE KNVVALEAHF PQAEKNVVAL 250 260 270 280 290 300 EGGCGPGNAL SLMIQSEQLT ELFAAFGVKG TSAEAVANQV AHEARRYLAS PAAVGEHLAD 310 320 330 340 350 QLILPLALAG EGAFTVARAS AHLLTNIVVV ERFLPVRFSC EATESGYLVR VSD

References

[1]	"Complete genome of Salmonella heidelberg strain SL476." Ravel J., Fricke W.F., White D., McDermott P., Mammel M., Rosovitz M., Leclerc J., Cebula T., Sebastian Y. Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001120 Genomic DNA. Translation: ACF69933.1.
RefSeq	YP_002047543.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6490448.
GenomeReviews	Gene locus SeHA_C3826 in contig CP001120_GR.
KEGG	seh:SeHA_C3826.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	EIDGSYG.
Family and domain databases
HAMAP	MF_00200. [Tree]
InterPro	IPR000228. RNA3'_term_phos_cycl-like. IPR013796. RNA3'_term_phos_cycl_insert. IPR017770. RNA3'_term_phos_cycl_sub. [Graphical view]
Gene3D	G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 1 hit.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_SALHS

Accession

Primary (citable) accession number: B4TKU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)