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B4TK70 (SYP_SALHS) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proline--tRNA ligase

EC=6.1.1.15
Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS
Gene names
Name:proS
Ordered Locus Names:SeHA_C0280
OrganismSalmonella heidelberg (strain SL476) [Complete proteome] [HAMAP]
Taxonomic identifier454169 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length572 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP-Rule MF_01569

Catalytic activity

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP-Rule MF_01569

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01569

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01569.

Domain

Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP-Rule MF_01569

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processprolyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

regulation of translational fidelity

Inferred from electronic annotation. Source: GOC

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

proline-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 572572Proline--tRNA ligase HAMAP-Rule MF_01569
PRO_1000199418

Sequences

Sequence LengthMass (Da)Tools
B4TK70 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: DCD34C0EFDE33CEC

FASTA57263,540
        10         20         30         40         50         60 
MRTSQYLLST LKETPADAEV ISHQLMLRAG MIRKLASGLY TWLPTGLRVL KKVENIVREE 

        70         80         90        100        110        120 
MNNAGAIEVS MPVVQPADLW QESGRWEQYG PELLRFVDRG ERPFVLGPTH EEVITDLVRN 

       130        140        150        160        170        180 
ELSSYKQLPL NFFQIQTKFR DEVRPRFGVM RSREFLMKDA YSFHTSQESL QETYDAMYAA 

       190        200        210        220        230        240 
YSRIFSRMGL DFRAVQADTG SIGGNASHEF QVLAQSGEDD IVFSDVSDYA ANIELAEAIA 

       250        260        270        280        290        300 
PQTPRAAATQ EMTLVDTPNA KTIAELVEQF NLPIEKTVKT LLVKAVKDSK SPLVALLVRG 

       310        320        330        340        350        360 
DHELNEVKAE KLPHVASPLT FATEEEIRAV INAGPGSLGP VNMPIPVIID RTVAAMSDFA 

       370        380        390        400        410        420 
AGANIDGKHY FGINWDRDVA TPVVADIRNV VAGDPSPDGQ GTLLIKRGIE VGHIFQLGTK 

       430        440        450        460        470        480 
YSEALKASVQ GEDGRNQILT MGCYGIGVTR VVAAAIEQNF DERGIVWPDA IAPFQVAILP 

       490        500        510        520        530        540 
MNMHKSFRVQ ELAEKLYSEL RAQGIEVLMD DRKERPGVMF ADMELIGIPH TIVIGDRNLD 

       550        560        570 
NDDIEYKYRR SGEKSLIKTG DIVDYLVKAI KG 

« Hide

References

[1]"Complete genome of Salmonella heidelberg strain SL476."
Ravel J., Fricke W.F., White D., McDermott P., Mammel M., Rosovitz M., Leclerc J., Cebula T., Sebastian Y.
Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SL476.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001120 Genomic DNA. Translation: ACF68125.1.
RefSeqYP_002044232.1. NC_011083.1.

3D structure databases

ProteinModelPortalB4TK70.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING454169.SeHA_C0280.

Proteomic databases

PRIDEB4TK70.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACF68125; ACF68125; SeHA_C0280.
PATRIC18508412. VBISalEnt43179_0338.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0442.
HOGENOMHOG000076894.
OMAIQPAELW.
OrthoDBEOG6TTVMR.
ProtClustDBPRK09194.

Enzyme and pathway databases

BioCycSENT454169:GHYG-273-MONOMER.

Family and domain databases

Gene3D3.40.50.800. 1 hit.
3.90.960.10. 1 hit.
HAMAPMF_01569. Pro_tRNA_synth_type1.
InterProIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023717. Pro-tRNA-Synthase_IIa_type1.
IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
[Graphical view]
PANTHERPTHR11451:SF3. PTHR11451:SF3. 1 hit.
PfamPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF04073. tRNA_edit. 1 hit.
[Graphical view]
PRINTSPR01046. TRNASYNTHPRO.
SUPFAMSSF52954. SSF52954. 1 hit.
SSF55826. SSF55826. 1 hit.
TIGRFAMsTIGR00409. proS_fam_II. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYP_SALHS
AccessionPrimary (citable) accession number: B4TK70
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 23, 2008
Last modified: February 19, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries