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B4TK70

- SYP_SALHS

UniProt

B4TK70 - SYP_SALHS

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Protein

Proline--tRNA ligase

Gene

proS

Organism
Salmonella heidelberg (strain SL476)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS.UniRule annotation

Catalytic activityi

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. proline-tRNA ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. prolyl-tRNA aminoacylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSENT454169:GHYG-273-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Proline--tRNA ligaseUniRule annotation (EC:6.1.1.15UniRule annotation)
Alternative name(s):
Prolyl-tRNA synthetaseUniRule annotation
Short name:
ProRSUniRule annotation
Gene namesi
Name:proSUniRule annotation
Ordered Locus Names:SeHA_C0280
OrganismiSalmonella heidelberg (strain SL476)
Taxonomic identifieri454169 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001866: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 572572Proline--tRNA ligasePRO_1000199418Add
BLAST

Proteomic databases

PRIDEiB4TK70.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi454169.SeHA_C0280.

Structurei

3D structure databases

ProteinModelPortaliB4TK70.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain.UniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0442.
HOGENOMiHOG000076894.
OMAiMHQAYCN.
OrthoDBiEOG6TTVMR.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
3.90.960.10. 1 hit.
HAMAPiMF_01569. Pro_tRNA_synth_type1.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023717. Pro-tRNA-Synthase_IIa_type1.
IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
[Graphical view]
PANTHERiPTHR11451:SF3. PTHR11451:SF3. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF04073. tRNA_edit. 1 hit.
[Graphical view]
PRINTSiPR01046. TRNASYNTHPRO.
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55826. SSF55826. 1 hit.
TIGRFAMsiTIGR00409. proS_fam_II. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4TK70-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRTSQYLLST LKETPADAEV ISHQLMLRAG MIRKLASGLY TWLPTGLRVL
60 70 80 90 100
KKVENIVREE MNNAGAIEVS MPVVQPADLW QESGRWEQYG PELLRFVDRG
110 120 130 140 150
ERPFVLGPTH EEVITDLVRN ELSSYKQLPL NFFQIQTKFR DEVRPRFGVM
160 170 180 190 200
RSREFLMKDA YSFHTSQESL QETYDAMYAA YSRIFSRMGL DFRAVQADTG
210 220 230 240 250
SIGGNASHEF QVLAQSGEDD IVFSDVSDYA ANIELAEAIA PQTPRAAATQ
260 270 280 290 300
EMTLVDTPNA KTIAELVEQF NLPIEKTVKT LLVKAVKDSK SPLVALLVRG
310 320 330 340 350
DHELNEVKAE KLPHVASPLT FATEEEIRAV INAGPGSLGP VNMPIPVIID
360 370 380 390 400
RTVAAMSDFA AGANIDGKHY FGINWDRDVA TPVVADIRNV VAGDPSPDGQ
410 420 430 440 450
GTLLIKRGIE VGHIFQLGTK YSEALKASVQ GEDGRNQILT MGCYGIGVTR
460 470 480 490 500
VVAAAIEQNF DERGIVWPDA IAPFQVAILP MNMHKSFRVQ ELAEKLYSEL
510 520 530 540 550
RAQGIEVLMD DRKERPGVMF ADMELIGIPH TIVIGDRNLD NDDIEYKYRR
560 570
SGEKSLIKTG DIVDYLVKAI KG
Length:572
Mass (Da):63,540
Last modified:September 23, 2008 - v1
Checksum:iDCD34C0EFDE33CEC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001120 Genomic DNA. Translation: ACF68125.1.
RefSeqiYP_002044232.1. NC_011083.1.

Genome annotation databases

EnsemblBacteriaiACF68125; ACF68125; SeHA_C0280.
PATRICi18508412. VBISalEnt43179_0338.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001120 Genomic DNA. Translation: ACF68125.1 .
RefSeqi YP_002044232.1. NC_011083.1.

3D structure databases

ProteinModelPortali B4TK70.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 454169.SeHA_C0280.

Proteomic databases

PRIDEi B4TK70.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACF68125 ; ACF68125 ; SeHA_C0280 .
PATRICi 18508412. VBISalEnt43179_0338.

Phylogenomic databases

eggNOGi COG0442.
HOGENOMi HOG000076894.
OMAi MHQAYCN.
OrthoDBi EOG6TTVMR.

Enzyme and pathway databases

BioCyci SENT454169:GHYG-273-MONOMER.

Family and domain databases

Gene3Di 3.40.50.800. 1 hit.
3.90.960.10. 1 hit.
HAMAPi MF_01569. Pro_tRNA_synth_type1.
InterProi IPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023717. Pro-tRNA-Synthase_IIa_type1.
IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
[Graphical view ]
PANTHERi PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfami PF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF04073. tRNA_edit. 1 hit.
[Graphical view ]
PRINTSi PR01046. TRNASYNTHPRO.
SUPFAMi SSF52954. SSF52954. 1 hit.
SSF55826. SSF55826. 1 hit.
TIGRFAMsi TIGR00409. proS_fam_II. 1 hit.
PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comparative genomics of 28 Salmonella enterica isolates: evidence for CRISPR-mediated adaptive sublineage evolution."
    Fricke W.F., Mammel M.K., McDermott P.F., Tartera C., White D.G., Leclerc J.E., Ravel J., Cebula T.A.
    J. Bacteriol. 193:3556-3568(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SL476.

Entry informationi

Entry nameiSYP_SALHS
AccessioniPrimary (citable) accession number: B4TK70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 23, 2008
Last modified: October 29, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3