B4TK70 (SYP_SALHS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	SeHA_C0280

Organism

Salmonella heidelberg (strain SL476) [Complete proteome] [HAMAP]

Taxonomic identifier

454169 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella enterica subsp. enterica serovar Heidelberg

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP-Rule MF_01569
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP-Rule MF_01569
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP-Rule MF_01569
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 572	572	Proline--tRNA ligase HAMAP-Rule MF_01569		PRO_1000199418

Sequences

	Sequence		Length	Mass (Da)
	B4TK70 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: DCD34C0EFDE33CEC	FASTA	572	63,540
	10 20 30 40 50 60 MRTSQYLLST LKETPADAEV ISHQLMLRAG MIRKLASGLY TWLPTGLRVL KKVENIVREE 70 80 90 100 110 120 MNNAGAIEVS MPVVQPADLW QESGRWEQYG PELLRFVDRG ERPFVLGPTH EEVITDLVRN 130 140 150 160 170 180 ELSSYKQLPL NFFQIQTKFR DEVRPRFGVM RSREFLMKDA YSFHTSQESL QETYDAMYAA 190 200 210 220 230 240 YSRIFSRMGL DFRAVQADTG SIGGNASHEF QVLAQSGEDD IVFSDVSDYA ANIELAEAIA 250 260 270 280 290 300 PQTPRAAATQ EMTLVDTPNA KTIAELVEQF NLPIEKTVKT LLVKAVKDSK SPLVALLVRG 310 320 330 340 350 360 DHELNEVKAE KLPHVASPLT FATEEEIRAV INAGPGSLGP VNMPIPVIID RTVAAMSDFA 370 380 390 400 410 420 AGANIDGKHY FGINWDRDVA TPVVADIRNV VAGDPSPDGQ GTLLIKRGIE VGHIFQLGTK 430 440 450 460 470 480 YSEALKASVQ GEDGRNQILT MGCYGIGVTR VVAAAIEQNF DERGIVWPDA IAPFQVAILP 490 500 510 520 530 540 MNMHKSFRVQ ELAEKLYSEL RAQGIEVLMD DRKERPGVMF ADMELIGIPH TIVIGDRNLD 550 560 570 NDDIEYKYRR SGEKSLIKTG DIVDYLVKAI KG

References

[1]	"Complete genome of Salmonella heidelberg strain SL476." Ravel J., Fricke W.F., White D., McDermott P., Mammel M., Rosovitz M., Leclerc J., Cebula T., Sebastian Y. Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SL476.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001120 Genomic DNA. Translation: ACF68125.1.
RefSeq	YP_002044232.1. NC_011083.1.
3D structure databases
ProteinModelPortal	B4TK70.
ModBase	Search...
Protein-protein interaction databases
STRING	454169.SeHA_C0280.
Proteomic databases
PRIDE	B4TK70.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACF68125; ACF68125; SeHA_C0280.
GeneID	6492184.
KEGG	seh:SeHA_C0280.
PATRIC	18508412. VBISalEnt43179_0338.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0442.
HOGENOM	HOG000076894.
KO	K01881.
OMA	IQPAELW.
ProtClustDB	PRK09194.
Enzyme and pathway databases
BioCyc	SENT454169:GHYG-2382-MONOMER.
Family and domain databases
Gene3D	3.40.50.800. 1 hit. 3.90.960.10. 1 hit.
HAMAP	MF_01569. Pro_tRNA_synth_type1.
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-ligase_IIa. IPR004500. Pro-tRNA-synth_IIa_bac-type. IPR023717. Pro-tRNA-Synthase_IIa_type1. IPR007214. YbaK/aa-tRNA-synth-assoc-dom. [Graphical view]
PANTHER	PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF55826. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
TIGRFAMs	TIGR00409. proS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_SALHS

Accession

Primary (citable) accession number: B4TK70

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 23, 2008
Last modified:	May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program