B4TJA0 (GUAC_SALHS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP reductase
EC=1.7.1.7

Alternative name(s):
Guanosine 5'-monophosphate oxidoreductase
Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	SeHA_C0155

Organism

Salmonella heidelberg (strain SL476) [Complete proteome] [HAMAP]

Taxonomic identifier

454169 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella enterica subsp. enterica serovar Heidelberg

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity. HAMAP MF_00596
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP MF_00596
Subunit structure	Homotetramer By similarity. HAMAP MF_00596
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 1 subfamily.

Ontologies

Keywords
Ligand	Metal-binding NADP Potassium
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	GMP reductase HAMAP MF_00596		PRO_1000129864
Regions
	Nucleotide binding	108 – 131	24	NADP By similarity
	Nucleotide binding	216 – 239	24	NADP; ribose moiety By similarity
Sites
	Active site	186	1	Thioimidate intermediate By similarity
	Metal binding	181	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	183	1	Potassium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4TJA0 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 71223D342F001B45	FASTA	347	37,139
	10 20 30 40 50 60 MRIEEDLKLG FKDVLIRPKR STLKSRSDVE LERQFTFKHS GQTWSGVPII AANMDTVGTF 70 80 90 100 110 120 EMAQALAGFD ILTAVHKHYT VEEWAAFINT ASADVLKHVM VSTGTSDADF EKTVQILALN 130 140 150 160 170 180 PALNFVCIDV ANGYSEHFVQ FVAKAREAWP TKTICAGNVV TGEMCEELIL SGADIVKVGI 190 200 210 220 230 240 GPGSVCTTRV KTGVGYPQLS AVIECADAAH GLGGMIVSDG GCTMPGDVAK AFGGGADFVM 250 260 270 280 290 300 LGGMLAGHEE SGGSVVEENG EKFMLFYGMS SESAMNRHVG GVAKYRAAEG KTVKLPLRGP 310 320 330 340 VGNTARDILG GLRSACTYVG ASRLKELTKR TTFIRVQEQE NRIFNSL

References

[1]	"Complete genome of Salmonella heidelberg strain SL476." Ravel J., Fricke W.F., White D., McDermott P., Mammel M., Rosovitz M., Leclerc J., Cebula T., Sebastian Y. Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SL476.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001120 Genomic DNA. Translation: ACF69085.1.
RefSeq	YP_002044111.1. NC_011083.1.
3D structure databases
ProteinModelPortal	B4TJA0.
SMR	B4TJA0. Positions 3-338.
ModBase	Search...
Protein-protein interaction databases
STRING	B4TJA0.
Proteomic databases
PRIDE	B4TJA0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6489724.
GenomeReviews	Gene locus SeHA_C0155 in contig CP001120_GR.
KEGG	seh:SeHA_C0155.
PATRIC	18508179. VBISalEnt43179_0222.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG298985.
OMA	FRNSKQM.
ProtClustDB	PRK05096.
Family and domain databases
HAMAP	MF_00596. GMP_reduct_type1. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005993. GMP_reduct1. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K00364.
PANTHER	PTHR11911:SF33. PTHR11911:SF33. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF000235. GMP_reductase. 1 hit.
TIGRFAMs	TIGR01305. GMP_reduct_1. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_SALHS

Accession

Primary (citable) accession number: B4TJA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program