B4TDY8 (RLMKL_SALHS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase K/L

Including the following 2 domains:

1. 23S rRNA m2G2445 methyltransferase
   EC=2.1.1.173
   Alternative name(s):
   rRNA (guanine-N(2)-)-methyltransferase RlmL
2. 23S rRNA m7G2069 methyltransferase
   EC=2.1.1.264
   Alternative name(s):
   rRNA (guanine-N(7)-)-methyltransferase RlmK

Gene names

Name:	rlmL
Ordered Locus Names:	SeHA_C1170

Organism

Salmonella heidelberg (strain SL476) [Complete proteome] [HAMAP]

Taxonomic identifier

454169 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella enterica subsp. enterica serovar Heidelberg

Protein attributes

Sequence length	702 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the guanine in position 2445 (m2G2445) and the guanine in position 2069 (m7G2069) of 23S rRNA By similarity. HAMAP-Rule MF_01858
Catalytic activity	S-adenosyl-L-methionine + guanine(2445) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(2445) in 23S rRNA. HAMAP-Rule MF_01858 S-adenosyl-L-methionine + guanine(2069) in 23S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(2069) in 23S rRNA. HAMAP-Rule MF_01858
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01858.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmKL family. Contains 1 THUMP domain.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	rRNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	23S rRNA (guanine(2445)-N(2))-methyltransferase activity Inferred from electronic annotation. Source: HAMAP RNA binding Inferred from electronic annotation. Source: HAMAP rRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 702	702	Ribosomal RNA large subunit methyltransferase K/L HAMAP-Rule MF_01858		PRO_0000366810
Regions
	Domain	43 – 154	112	THUMP

Sequences

	Sequence		Length	Mass (Da)
	B4TDY8 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 031E82BCA744E152	FASTA	702	78,849
	10 20 30 40 50 60 MNSLFASTAR GLEELLKTEL EKLGAVGCQV VQGGVHFQGD TRLIYQSLMW SRLASRIILP 70 80 90 100 110 120 MGECKVYSDL DLYLGVQAIN WTEIFNPGAT FAVHFSGLND TIRNSQYGAM KVKDAIVDAF 130 140 150 160 170 180 TRKNLPRPNV DRESPDLRIN VWLNKETASI ALDLSGDGLH LRGYRDRTGL APIKETLAAA 190 200 210 220 230 240 IVMRSGWQPG TPLLDPMCGS GTLLIEAAMW ATDRAPGLHR GHWGFSGWAQ HDETIWQEVK 250 260 270 280 290 300 AEAQTRARKG LAEYSSHFYG SDSDARVIER ARSNARRAGI GELITFEVKD VAQLSNPLPK 310 320 330 340 350 360 GPYGTVISNP PYGERLDSEP ALIALHSLLG RTMKNQFGGW NLSLFSASPD LLGSLQLRAD 370 380 390 400 410 420 KQFKAKNGPL DCVQKNYHIA ETTADSKPAT VAEDYANRLR KNLKKLEKWA RQEGIECYRL 430 440 450 460 470 480 YDADLPEYNV AVDRYGDWAV IQEYAPPKTV DAQKARQRLF DIIAATLSVL GIPPNKLVLK 490 500 510 520 530 540 TRERQKGKNQ YQKMSEKGEF LEVSEYNARL WVNLTDYLDT GLFLDHRIAR RMLGEMSKGK 550 560 570 580 590 600 DFLNLFSYTG SASVHAGLGG ARSTTTVDMS RTYLEWAERN LRLNGLSGRA HRLIQADCLG 610 620 630 640 650 660 WLREANEQFD LIFIDPPTFS NSKRMEESFD VQRDHVALMK DLKRLLRKGG TIMFSNNKRG 670 680 690 700 FRMDLEGLAE LGLTAQEITQ KTLSPDFARN RQIHNCWLIR AA

References

[1]	"Complete genome of Salmonella heidelberg strain SL476." Ravel J., Fricke W.F., White D., McDermott P., Mammel M., Rosovitz M., Leclerc J., Cebula T., Sebastian Y. Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SL476.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001120 Genomic DNA. Translation: ACF69678.1.
RefSeq	YP_002045063.1. NC_011083.1.
3D structure databases
ProteinModelPortal	B4TDY8.
ModBase	Search...
Protein-protein interaction databases
STRING	454169.SeHA_C1170.
Proteomic databases
PRIDE	B4TDY8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACF69678; ACF69678; SeHA_C1170.
GeneID	6488811.
KEGG	seh:SeHA_C1170.
PATRIC	18510082. VBISalEnt43179_1152.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0116.
HOGENOM	HOG000218371.
KO	K12297.
OMA	NKRGFKM.
ProtClustDB	PRK11783.
Enzyme and pathway databases
BioCyc	SENT454169:GHYG-3983-MONOMER.
Family and domain databases
HAMAP	MF_01858. 23SrRNA_methyltr_KL.
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR002296. N12N6_MeTrfase. IPR000241. RNA_methylase_dom. IPR017244. S-Ado-dep_RNA_MeTrfase_bac. IPR019614. SAM-dep_methyl-trfase. IPR004114. THUMP. [Graphical view]
Pfam	PF10672. Methyltrans_SAM. 1 hit. PF02926. THUMP. 1 hit. PF01170. UPF0020. 1 hit. [Graphical view]
PIRSF	PIRSF037618. RNA_Mtase_bacteria_prd. 1 hit.
PRINTS	PR00507. N12N6MTFRASE.
SMART	SM00981. THUMP. 1 hit. [Graphical view]
PROSITE	PS51165. THUMP. 1 hit. PS01261. UPF0020. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RLMKL_SALHS

Accession

Primary (citable) accession number: B4TDY8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program