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Protein

Ribosomal RNA large subunit methyltransferase K/L

Gene

rlmL

Organism
Salmonella heidelberg (strain SL476)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the guanine in position 2445 (m2G2445) and the guanine in position 2069 (m7G2069) of 23S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(2445) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(2445) in 23S rRNA.UniRule annotation
S-adenosyl-L-methionine + guanine(2069) in 23S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(2069) in 23S rRNA.UniRule annotation

GO - Molecular functioni

  1. 23S rRNA (guanine(2445)-N(2))-methyltransferase activity Source: UniProtKB-HAMAP
  2. RNA binding Source: UniProtKB-HAMAP
  3. rRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSENT454169:GHYG-1160-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA large subunit methyltransferase K/LUniRule annotation
Including the following 2 domains:
23S rRNA m2G2445 methyltransferaseUniRule annotation (EC:2.1.1.173UniRule annotation)
Alternative name(s):
rRNA (guanine-N(2)-)-methyltransferase RlmLUniRule annotation
23S rRNA m7G2069 methyltransferaseUniRule annotation (EC:2.1.1.264UniRule annotation)
Alternative name(s):
rRNA (guanine-N(7)-)-methyltransferase RlmKUniRule annotation
Gene namesi
Name:rlmLUniRule annotation
Ordered Locus Names:SeHA_C1170
OrganismiSalmonella heidelberg (strain SL476)
Taxonomic identifieri454169 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001866: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 702702Ribosomal RNA large subunit methyltransferase K/LPRO_0000366810Add
BLAST

Proteomic databases

PRIDEiB4TDY8.

Interactioni

Protein-protein interaction databases

STRINGi454169.SeHA_C1170.

Structurei

3D structure databases

ProteinModelPortaliB4TDY8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 154112THUMPUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the methyltransferase superfamily. RlmKL family.UniRule annotation
Contains 1 THUMP domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0116.
HOGENOMiHOG000218371.
OMAiGYEADPR.
OrthoDBiEOG6HJ238.

Family and domain databases

Gene3Di3.40.50.150. 3 hits.
HAMAPiMF_01858. 23SrRNA_methyltr_KL.
InterProiIPR017244. 23SrRNA_methyltr_KL.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR000241. RNA_methylase_dom.
IPR019614. SAM-dep_methyl-trfase.
IPR029063. SAM-dependent_MTases.
IPR004114. THUMP.
[Graphical view]
PfamiPF10672. Methyltrans_SAM. 1 hit.
PF02926. THUMP. 1 hit.
PF01170. UPF0020. 1 hit.
[Graphical view]
PIRSFiPIRSF037618. RNA_Mtase_bacteria_prd. 1 hit.
PRINTSiPR00507. N12N6MTFRASE.
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 3 hits.
PROSITEiPS51165. THUMP. 1 hit.
PS01261. UPF0020. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4TDY8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNSLFASTAR GLEELLKTEL EKLGAVGCQV VQGGVHFQGD TRLIYQSLMW
60 70 80 90 100
SRLASRIILP MGECKVYSDL DLYLGVQAIN WTEIFNPGAT FAVHFSGLND
110 120 130 140 150
TIRNSQYGAM KVKDAIVDAF TRKNLPRPNV DRESPDLRIN VWLNKETASI
160 170 180 190 200
ALDLSGDGLH LRGYRDRTGL APIKETLAAA IVMRSGWQPG TPLLDPMCGS
210 220 230 240 250
GTLLIEAAMW ATDRAPGLHR GHWGFSGWAQ HDETIWQEVK AEAQTRARKG
260 270 280 290 300
LAEYSSHFYG SDSDARVIER ARSNARRAGI GELITFEVKD VAQLSNPLPK
310 320 330 340 350
GPYGTVISNP PYGERLDSEP ALIALHSLLG RTMKNQFGGW NLSLFSASPD
360 370 380 390 400
LLGSLQLRAD KQFKAKNGPL DCVQKNYHIA ETTADSKPAT VAEDYANRLR
410 420 430 440 450
KNLKKLEKWA RQEGIECYRL YDADLPEYNV AVDRYGDWAV IQEYAPPKTV
460 470 480 490 500
DAQKARQRLF DIIAATLSVL GIPPNKLVLK TRERQKGKNQ YQKMSEKGEF
510 520 530 540 550
LEVSEYNARL WVNLTDYLDT GLFLDHRIAR RMLGEMSKGK DFLNLFSYTG
560 570 580 590 600
SASVHAGLGG ARSTTTVDMS RTYLEWAERN LRLNGLSGRA HRLIQADCLG
610 620 630 640 650
WLREANEQFD LIFIDPPTFS NSKRMEESFD VQRDHVALMK DLKRLLRKGG
660 670 680 690 700
TIMFSNNKRG FRMDLEGLAE LGLTAQEITQ KTLSPDFARN RQIHNCWLIR

AA
Length:702
Mass (Da):78,849
Last modified:September 23, 2008 - v1
Checksum:i031E82BCA744E152
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001120 Genomic DNA. Translation: ACF69678.1.
RefSeqiYP_002045063.1. NC_011083.1.

Genome annotation databases

EnsemblBacteriaiACF69678; ACF69678; SeHA_C1170.
PATRICi18510082. VBISalEnt43179_1152.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001120 Genomic DNA. Translation: ACF69678.1.
RefSeqiYP_002045063.1. NC_011083.1.

3D structure databases

ProteinModelPortaliB4TDY8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi454169.SeHA_C1170.

Proteomic databases

PRIDEiB4TDY8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACF69678; ACF69678; SeHA_C1170.
PATRICi18510082. VBISalEnt43179_1152.

Phylogenomic databases

eggNOGiCOG0116.
HOGENOMiHOG000218371.
OMAiGYEADPR.
OrthoDBiEOG6HJ238.

Enzyme and pathway databases

BioCyciSENT454169:GHYG-1160-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 3 hits.
HAMAPiMF_01858. 23SrRNA_methyltr_KL.
InterProiIPR017244. 23SrRNA_methyltr_KL.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR000241. RNA_methylase_dom.
IPR019614. SAM-dep_methyl-trfase.
IPR029063. SAM-dependent_MTases.
IPR004114. THUMP.
[Graphical view]
PfamiPF10672. Methyltrans_SAM. 1 hit.
PF02926. THUMP. 1 hit.
PF01170. UPF0020. 1 hit.
[Graphical view]
PIRSFiPIRSF037618. RNA_Mtase_bacteria_prd. 1 hit.
PRINTSiPR00507. N12N6MTFRASE.
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 3 hits.
PROSITEiPS51165. THUMP. 1 hit.
PS01261. UPF0020. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Comparative genomics of 28 Salmonella enterica isolates: evidence for CRISPR-mediated adaptive sublineage evolution."
    Fricke W.F., Mammel M.K., McDermott P.F., Tartera C., White D.G., Leclerc J.E., Ravel J., Cebula T.A.
    J. Bacteriol. 193:3556-3568(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SL476.

Entry informationi

Entry nameiRLMKL_SALHS
AccessioniPrimary (citable) accession number: B4TDY8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: January 7, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.