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B4TDY8 (RLMKL_SALHS) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosomal RNA large subunit methyltransferase K/L

Including the following 2 domains:

  1. 23S rRNA m2G2445 methyltransferase
    EC=2.1.1.173
    Alternative name(s):
    rRNA (guanine-N(2)-)-methyltransferase RlmL
  2. 23S rRNA m7G2069 methyltransferase
    EC=2.1.1.264
    Alternative name(s):
    rRNA (guanine-N(7)-)-methyltransferase RlmK
Gene names
Name:rlmL
Ordered Locus Names:SeHA_C1170
OrganismSalmonella heidelberg (strain SL476) [Complete proteome] [HAMAP]
Taxonomic identifier454169 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length702 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates the guanine in position 2445 (m2G2445) and the guanine in position 2069 (m7G2069) of 23S rRNA By similarity. HAMAP-Rule MF_01858

Catalytic activity

S-adenosyl-L-methionine + guanine(2445) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(2445) in 23S rRNA. HAMAP-Rule MF_01858

S-adenosyl-L-methionine + guanine(2069) in 23S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(2069) in 23S rRNA. HAMAP-Rule MF_01858

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_01858.

Sequence similarities

Belongs to the methyltransferase superfamily. RlmKL family.

Contains 1 THUMP domain.

Ontologies

Keywords
   Biological processrRNA processing
   Cellular componentCytoplasm
   LigandRNA-binding
S-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function23S rRNA (guanine(2445)-N(2))-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

RNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

rRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 702702Ribosomal RNA large subunit methyltransferase K/L HAMAP-Rule MF_01858
PRO_0000366810

Regions

Domain43 – 154112THUMP

Sequences

Sequence LengthMass (Da)Tools
B4TDY8 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: 031E82BCA744E152

FASTA70278,849
        10         20         30         40         50         60 
MNSLFASTAR GLEELLKTEL EKLGAVGCQV VQGGVHFQGD TRLIYQSLMW SRLASRIILP 

        70         80         90        100        110        120 
MGECKVYSDL DLYLGVQAIN WTEIFNPGAT FAVHFSGLND TIRNSQYGAM KVKDAIVDAF 

       130        140        150        160        170        180 
TRKNLPRPNV DRESPDLRIN VWLNKETASI ALDLSGDGLH LRGYRDRTGL APIKETLAAA 

       190        200        210        220        230        240 
IVMRSGWQPG TPLLDPMCGS GTLLIEAAMW ATDRAPGLHR GHWGFSGWAQ HDETIWQEVK 

       250        260        270        280        290        300 
AEAQTRARKG LAEYSSHFYG SDSDARVIER ARSNARRAGI GELITFEVKD VAQLSNPLPK 

       310        320        330        340        350        360 
GPYGTVISNP PYGERLDSEP ALIALHSLLG RTMKNQFGGW NLSLFSASPD LLGSLQLRAD 

       370        380        390        400        410        420 
KQFKAKNGPL DCVQKNYHIA ETTADSKPAT VAEDYANRLR KNLKKLEKWA RQEGIECYRL 

       430        440        450        460        470        480 
YDADLPEYNV AVDRYGDWAV IQEYAPPKTV DAQKARQRLF DIIAATLSVL GIPPNKLVLK 

       490        500        510        520        530        540 
TRERQKGKNQ YQKMSEKGEF LEVSEYNARL WVNLTDYLDT GLFLDHRIAR RMLGEMSKGK 

       550        560        570        580        590        600 
DFLNLFSYTG SASVHAGLGG ARSTTTVDMS RTYLEWAERN LRLNGLSGRA HRLIQADCLG 

       610        620        630        640        650        660 
WLREANEQFD LIFIDPPTFS NSKRMEESFD VQRDHVALMK DLKRLLRKGG TIMFSNNKRG 

       670        680        690        700 
FRMDLEGLAE LGLTAQEITQ KTLSPDFARN RQIHNCWLIR AA 

« Hide

References

[1]"Complete genome of Salmonella heidelberg strain SL476."
Ravel J., Fricke W.F., White D., McDermott P., Mammel M., Rosovitz M., Leclerc J., Cebula T., Sebastian Y.
Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SL476.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001120 Genomic DNA. Translation: ACF69678.1.
RefSeqYP_002045063.1. NC_011083.1.

3D structure databases

ProteinModelPortalB4TDY8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING454169.SeHA_C1170.

Proteomic databases

PRIDEB4TDY8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACF69678; ACF69678; SeHA_C1170.
PATRIC18510082. VBISalEnt43179_1152.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0116.
HOGENOMHOG000218371.
OMANKRGFKM.
OrthoDBEOG6HJ238.
ProtClustDBPRK11783.

Enzyme and pathway databases

BioCycSENT454169:GHYG-1160-MONOMER.

Family and domain databases

HAMAPMF_01858. 23SrRNA_methyltr_KL.
InterProIPR017244. 23SrRNA_methyltr_KL.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR000241. RNA_methylase_dom.
IPR019614. SAM-dep_methyl-trfase.
IPR004114. THUMP.
[Graphical view]
PfamPF10672. Methyltrans_SAM. 1 hit.
PF02926. THUMP. 1 hit.
PF01170. UPF0020. 1 hit.
[Graphical view]
PIRSFPIRSF037618. RNA_Mtase_bacteria_prd. 1 hit.
PRINTSPR00507. N12N6MTFRASE.
SMARTSM00981. THUMP. 1 hit.
[Graphical view]
PROSITEPS51165. THUMP. 1 hit.
PS01261. UPF0020. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRLMKL_SALHS
AccessionPrimary (citable) accession number: B4TDY8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: April 16, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families