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B4TCA8 (FADJ_SALHS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Fatty acid oxidation complex subunit alpha

Including the following 2 domains:

  1. Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase
    EC=4.2.1.17
    EC=5.1.2.3
  2. 3-hydroxyacyl-CoA dehydrogenase
    EC=1.1.1.35
Gene names
Name:fadJ
Ordered Locus Names:SeHA_C2630
OrganismSalmonella heidelberg (strain SL476) [Complete proteome] [HAMAP]
Taxonomic identifier454169 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella ›

Protein attributes

Sequence length715 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities By similarity. HAMAP-Rule MF_01617

Catalytic activity

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O. HAMAP-Rule MF_01617

(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH. HAMAP-Rule MF_01617

(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA. HAMAP-Rule MF_01617

Pathway

Lipid metabolism; fatty acid beta-oxidation. HAMAP-Rule MF_01617

Subunit structure

Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI) By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.

In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 715715Fatty acid oxidation complex subunit alpha HAMAP-Rule MF_01617
PRO_1000185951

Regions

Region1 – 190190Enoyl-CoA hydratase By similarity
Region306 – 7154103-hydroxyacyl-CoA dehydrogenase By similarity

Sites

Site1181Important for catalytic activity By similarity
Site1401Important for catalytic activity By similarity

Sequences

Sequence LengthMass (Da)Tools
B4TCA8 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: 6544646BA777687A

FASTA71577,211
        10         20         30         40         50         60 
MTTTSAFMLN VRLDNVAVVA IDVPGEKVNT LKAEFAAQVR AILKQIRENK ALQGVVFISA 

        70         80         90        100        110        120 
KADNFIAGAD INMIGHCQNA QEAETLARQG QQLMAEIQAL PVPVIAAIHG ACLGGGLEMA 

       130        140        150        160        170        180 
LACHRRICTD DVKTVLGLPE VQLGLLPGSG GTQRLPRLVG VSTALDMILT GKQLRARQAL 

       190        200        210        220        230        240 
KAGLVDDVVP QTILLEAAVE LAKKERLAQR TLPVRERILA GPLGRALLFR LVRKKTAQKT 

       250        260        270        280        290        300 
QGNYPATERI IDVIETGLAQ GSSSGYDAEA RAFGELAMTP QSQALRAVFF ASTEVKKDPG 

       310        320        330        340        350        360 
SDAPPGPLNS VGILGGGLMG GGIAWVTACK GGLPVRIKDI NTQGINHALK YSWDLLETKV 

       370        380        390        400        410        420 
RRRHIKASER DKQLALISGS TDYRGFSHRD LVIEAVFEDL PLKQQMVAEV EQNCAAHTIF 

       430        440        450        460        470        480 
ASNTSSLPIG DIAANAARPE QVIGLHFFSP VEKMPLVEVI PHASTSAQTI ATTVKLAKKQ 

       490        500        510        520        530        540 
GKTPIVVSDK AGFYVNRILA PYINEAIRML TEGERVEHID AALVKFGFPV GPIQLLDEVG 

       550        560        570        580        590        600 
IDTGTKIIPV LEAAYGERFS APANVVASIL NDDRKGRKNG RGFYLYGEKG RKSKKQVDPA 

       610        620        630        640        650        660 
IYKLIGVQGQ SRLSAQQVAE RCVMLMLNEA ARCFDEKVIR SARDGDIGAV FGIGFPPFLG 

       670        680        690        700        710 
GPFRYMDALG PGEMVATLQR LAALYGPRYA PCEQLVRMAE RREHFWTNGE TDQGN

« Hide

References

[1]"Complete genome of Salmonella heidelberg strain SL476."
Ravel J., Fricke W.F., White D., McDermott P., Mammel M., Rosovitz M., Leclerc J., Cebula T., Sebastian Y.
Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SL476.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001120 Genomic DNA. Translation: ACF69849.1.
RefSeqYP_002046443.1. NC_011083.1.

3D structure databases

ProteinModelPortalB4TCA8.
ModBaseSearch...

Protein-protein interaction databases

STRING454169.SeHA_C2630.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACF69849; ACF69849; SeHA_C2630.
GeneID6488772.
KEGGseh:SeHA_C2630.
PATRIC18512888. VBISalEnt43179_2542.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1250.
HOGENOMHOG000261346.
KOK01782.
OMASPKRDKG.
ProtClustDBPRK11154.

Enzyme and pathway databases

BioCycSENT454169:GHYG-4164-MONOMER.
UniPathwayUPA00659.

Family and domain databases

Gene3D1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
HAMAPMF_01617. FadJ.
InterProIPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR012802. FadJ.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF00725. 3HCDH. 1 hit.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMSSF48179. 6DGDH_C_like. 2 hits.
TIGRFAMsTIGR02440. FadJ. 1 hit.
PROSITEPS00067. 3HCDH. 1 hit.
PS00166. ENOYL_COA_HYDRATASE. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFADJ_SALHS
AccessionPrimary (citable) accession number: B4TCA8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 23, 2008
Last modified: May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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