B4TB84 (SYQ_SALHS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamine--tRNA ligase
EC=6.1.1.18

Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	SeHA_C0805

Organism

Salmonella heidelberg (strain SL476) [Complete proteome] [HAMAP]

Taxonomic identifier

454169 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella enterica subsp. enterica serovar Heidelberg

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP-Rule MF_00126
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00126
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00126.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: UniProtKB-HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: UniProtKB-HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 555	555	Glutamine--tRNA ligase HAMAP-Rule MF_00126		PRO_1000095512
Regions
	Motif	34 – 44	11	"HIGH" region HAMAP-Rule MF_00126
	Motif	268 – 272	5	"KMSKS" region HAMAP-Rule MF_00126
Sites
	Binding site	271	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4TB84 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 8F47D5CBF9577E64	FASTA	555	63,538
	10 20 30 40 50 60 MSEAEARPTN FIRQIIDEDL ASGKHTTVHT RFPPEPNGYL HIGHAKSICL NFGIAQDYQG 70 80 90 100 110 120 QCNLRFDDTN PVKEDIEYVD SIKNDVEWLG FHWSGDIRYS SDYFDQLHAY AVELINKGLA 130 140 150 160 170 180 YVDELTPEQI REYRGTLTAP GKNSPFRDRS VEENLALFEK MRTGGFEEGK ACLRAKIDMA 190 200 210 220 230 240 SPFIVMRDPV LYRIKFAEHH QTGNKWCIYP MYDFTHCISD ALEGITHSLC TLEFQDNRRL 250 260 270 280 290 300 YDWVLDNITI PVHPRQYEFS RLNLEYTVMS KRKLNLLVTD KHVEGWDDPR MPTISGLRRR 310 320 330 340 350 360 GYTAASIREF CKRIGVTKQD NTIEMASLES CIREDLNENA PRAMAVIDPV KLVIENYPQG 370 380 390 400 410 420 ESEMVTMPNH PNKPEMGSRE VPFSGEIWID RADFREEANK QYKRLVMGKE VRLRNAYVIK 430 440 450 460 470 480 AERVEKDAEG NITTIFCTYD ADTLSKDPAD GRKVKGVIHW VSAAHALPIE IRLYDRLFSV 490 500 510 520 530 540 PNPGAAEDFL SVINPESLVI KQGYGEPSLK AAVAGKAFQF EREGYFCLDS RYATADKLVF 550 NRTVGLRDTW AKAGE

References

[1]

"Comparative genomics of 28 Salmonella enterica isolates: evidence for CRISPR-mediated adaptive sublineage evolution." Fricke W.F., Mammel M.K., McDermott P.F., Tartera C., White D.G., Leclerc J.E., Ravel J., Cebula T.A. J. Bacteriol. 193:3556-3568(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SL476.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001120 Genomic DNA. Translation: ACF68510.1.
RefSeq	YP_002044715.1. NC_011083.1.
3D structure databases
ProteinModelPortal	B4TB84.
SMR	B4TB84. Positions 9-549.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
STRING	454169.SeHA_C0805.
Proteomic databases
PRIDE	B4TB84.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACF68510; ACF68510; SeHA_C0805.
PATRIC	18509398. VBISalEnt43179_0816.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000259232.
OMA	SREIYID.
OrthoDB	EOG6DRPF7.
Enzyme and pathway databases
BioCyc	SENT454169:GHYG-796-MONOMER.
Family and domain databases
Gene3D	1.10.1160.10. 1 hit. 2.40.240.10. 2 hits. 3.40.50.620. 2 hits.
HAMAP	MF_00126. Gln_tRNA_synth.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth. IPR022861. Gln_tRNA_ligase_bac. IPR000924. Glu/Gln-tRNA-synth. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. SSF50715. 1 hit.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_SALHS

Accession

Primary (citable) accession number: B4TB84

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	July 9, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program