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Reviewed, UniProtKB/Swiss-Prot B4T1X2 (NRFA_SALNS)

Last modified February 9, 2010. Version 12. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cytochrome c-552
    EC=1.7.2.2
Alternative name(s):
    Ammonia-forming cytochrome c nitrite reductase
      Short name=Cytochrome c nitrite reductase
Gene names
Name: nrfA
Ordered Locus Names: SNSL254_A4622
OrganismSalmonella newport (strain SL254) [Complete proteome] [HAMAP]
Taxonomic identifier423368 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella newport

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Protein attributes

Sequence length478 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Plays a role in nitrite reduction By similarity. HAMAP MF_01182

Catalytic activity

NH3 + 2 H2O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H+. HAMAP MF_01182

Cofactor

Binds 1 calcium ion per monomer By similarity. HAMAP MF_01182

Binds 5 heme groups covalently per monomer By similarity. HAMAP MF_01182

Pathway

Nitrogen metabolism; nitrate reduction (assimilation). HAMAP MF_01182

Subcellular location

Periplasm By similarity HAMAP MF_01182.

Sequence similarities

Belongs to the cytochrome c-552 family.

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Ontologies

Keywords
   Biological processElectron transport
Transport
   Cellular componentPeriplasm
   DomainSignal
   LigandCalcium
Heme
Iron
Metal-binding
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processelectron transport chain

Inferred from electronic annotation. Source: UniProtKB-KW

nitrogen compound metabolic process

Inferred from electronic annotation. Source: HAMAP

transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentperiplasmic space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

heme binding

Inferred from electronic annotation. Source: InterPro

nitrite reductase (cytochrome, ammonia-forming) activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 478452Cytochrome c-552 HAMAP MF_01182
PRO_1000138220

Sites

Metal binding941Iron (heme 3 axial ligand) By similarity
Metal binding1261Iron (heme 1 axial ligand) By similarity
Metal binding1641Iron (heme 2 axial ligand) By similarity
Metal binding2131Iron (heme 3 axial ligand) By similarity
Metal binding2151Calcium By similarity
Metal binding2161Calcium; via carbonyl oxygen By similarity
Metal binding2611Calcium; via carbonyl oxygen By similarity
Metal binding2631Calcium By similarity
Metal binding2751Iron (heme 5 axial ligand) By similarity
Metal binding2861Iron (heme 4 axial ligand) By similarity
Metal binding3011Iron (heme 2 axial ligand) By similarity
Metal binding3181Iron (heme 5 axial ligand) By similarity
Metal binding3931Iron (heme 4 axial ligand) By similarity
Binding site1221Heme 1 (covalent) By similarity
Binding site1251Heme 1 (covalent) By similarity
Binding site1601Heme 2 (covalent) By similarity
Binding site1631Heme 2 (covalent) By similarity
Binding site2091Heme 3 (covalent) By similarity
Binding site2121Heme 3 (covalent) By similarity
Binding site2161Substrate By similarity
Binding site2641Substrate By similarity
Binding site2821Heme 4 (covalent) By similarity
Binding site2851Heme 4 (covalent) By similarity
Binding site3141Heme 5 (covalent) By similarity
Binding site3171Heme 5 (covalent) By similarity

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Sequences

Sequence LengthMass (Da)Tools
B4T1X2-1 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: 0AD7521E66215571

FASTA47853,787
        10         20         30         40         50         60 
MARKTLRARR FFSLIFPFFF ITSVYAEQTP ESAKTVTVEA KNEMFAPQHP DQYQSWKATS 

        70         80         90        100        110        120 
EQSAREDALA EDPRLVILWA GYPFSRDYNK PRGHAYAVTD VRETLRTGAP KTAEDGPLPM 

       130        140        150        160        170        180 
ACWSCKSPDV ARLIQQEGED GYFHGKWARG GPEIVNDLGC ADCHNTASDD FAQGKPALTL 

       190        200        210        220        230        240 
SRPYAERAME AIGKPFDKAG RFDQQSMVCG QCHVEYYFDG KNKAVKFPWD EGMKVENMEQ 

       250        260        270        280        290        300 
YYDAIAFSDW TNSLSKTPML KAQHPEYETW SAGIHGKNNV TCIDCHMPKV QNAEGKLYTD 

       310        320        330        340        350        360 
HKIGNPFDNF AQTCANCHTQ DKASLQKVVA ERKQAIHDLK IKVEDQLVHA HFEAKAAWDA 

       370        380        390        400        410        420 
GATDAEMKPI LNDIRHAQWR WDLAIASHGI HMHAPEEGLR MLGSAMDKAA DARTKLARLL 

       430        440        450        460        470 
ATKGITHEIP LPDISTKEKA QKAIGLNMQQ INAEKQDFLK TVVPQWEDQA RKNGLLSQ

« Hide

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References

[1]"Complete genome of Salmonella newport strain SL254."
Ravel J., Fricke W.F., White D., McDermott P., Mammel M., Rosovitz M., Leclerc J., Cebula T., Sebastian Y.
Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001113 Genomic DNA. Translation: ACF62061.1.
RefSeqYP_002043528.1.

3D structure databases

SMRB4T1X2. Positions 37-477.
ModBaseSearch...

Genome annotation databases

GeneID6483130.
GenomeReviewsGene locus SNSL254_A4622 in contig CP001113_GR.
KEGGsee:SNSL254_A4622.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG488281.
OMAPWDMGTT.

Family and domain databases

HAMAPMF_01182. Cytochrom_C552.
[Tree]
InterProIPR003321. Cyt_c552.
IPR017570. Cyt_c_NO2Rdtase_formate-dep.
IPR011031. Multihaem_cyt.
[Graphical view]
PfamPF02335. Cytochrom_C552. 1 hit.
[Graphical view]
PIRSFPIRSF000243. Cyt_c552. 1 hit.
TIGRFAMsTIGR03152. cyto_c552_HCOOH. 1 hit.
PROSITEPS51008. MULTIHEME_CYTC. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameNRFA_SALNS
AccessionPrimary (citable) accession number: B4T1X2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 23, 2008
Last modified: February 9, 2010
This is version 12 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents