B4SZF9 (NADA_SALNS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Quinolinate synthase A
EC=2.5.1.72

Gene names

Name:	nadA
Ordered Locus Names:	SNSL254_A0820

Organism

Salmonella newport (strain SL254) [Complete proteome] [HAMAP]

Taxonomic identifier

423368 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella newport

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate By similarity. HAMAP-Rule MF_00567
Catalytic activity	Glycerone phosphate + iminosuccinate = pyridine-2,3-dicarboxylate + 2 H2O + phosphate. HAMAP-Rule MF_00567
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from iminoaspartate: step 1/1. HAMAP-Rule MF_00567
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the quinolinate synthase A family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP quinolinate biosynthetic process Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinolinate synthetase A activity Inferred from electronic annotation. Source: HAMAP transferase activity, transferring alkyl or aryl (other than methyl) groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Quinolinate synthase A HAMAP-Rule MF_00567		PRO_1000129426
Sites
	Binding site	47	1	Iminoaspartate By similarity
	Binding site	68	1	Iminoaspartate; via amide nitrogen By similarity
	Binding site	139	1	Iminoaspartate By similarity
	Binding site	156	1	Iminoaspartate By similarity
	Binding site	226	1	Iminoaspartate By similarity
	Binding site	243	1	Iminoaspartate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4SZF9 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: FD5AA227CC2ED057	FASTA	347	37,927
	10 20 30 40 50 60 MSVMFDPQAA IYPFPPKPTP LNDDEKQFYR EKIKRLLKER NAVMVAHYYT DPEIQQLAEE 70 80 90 100 110 120 TGGCISDSLE MARFGAKHAA STLLVAGVRF MGETAKILSP EKNILMPTLA AECSLDLGCP 130 140 150 160 170 180 IDEFSAFCDA HPDRTVVVYA NTSAAVKARA DWVVTSSIAV ELIEHLDSLG EKIIWAPDRH 190 200 210 220 230 240 LGNYVQKQTG ADVLCWQGAC IVHDEFKTQA LTRLKKIYPD AALLVHPESP QSIVEMADAV 250 260 270 280 290 300 GSTSQLIKAA KTLPHRQLIV ATDRGIFYKM QQAVPEKELL EAPTAGEGAT CRSCAHCPWM 310 320 330 340 AMNGLKAIAE GLEQGGAAHE IQVDAALREG ALLPLNRMLD FAATLRA

References

[1]	"Complete genome of Salmonella newport strain SL254." Ravel J., Fricke W.F., White D., McDermott P., Mammel M., Rosovitz M., Leclerc J., Cebula T., Sebastian Y. Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SL254.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001113 Genomic DNA. Translation: ACF62134.1.
RefSeq	YP_002039992.1. NC_011080.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	423368.SNSL254_A0820.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACF62134; ACF62134; SNSL254_A0820.
GeneID	6487023.
KEGG	see:SNSL254_A0820.
PATRIC	18520062. VBISalEnt114557_0936.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0379.
HOGENOM	HOG000222769.
KO	K03517.
OMA	GCPADEF.
ProtClustDB	PRK09375.
Enzyme and pathway databases
BioCyc	SENT423368:GHJB-1075-MONOMER.
UniPathway	UPA00253; UER00327.
Family and domain databases
HAMAP	MF_00567. NadA_type1.
InterPro	IPR003473. NadA. IPR023513. Quinolinate_synth_A_type1. [Graphical view]
Pfam	PF02445. NadA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00550. nadA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADA_SALNS

Accession

Primary (citable) accession number: B4SZF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 23, 2008
Last modified:	May 1, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program