true2009-03-242024-03-2777TGT_SALNSComparative genomics of 28 Salmonella enterica isolates: evidence for CRISPR-mediated adaptive sublineage evolution.Fricke W.F.Mammel M.K.McDermott P.F.Tartera C.White D.G.Leclerc J.E.Ravel J.Cebula T.A.doi:10.1128/jb.00297-112011J. Bacteriol.1933556-3568NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]SL254Queuine tRNA-ribosyltransferase-likeQ_tRNA_TgtTGTTGT-like_sftRNA_ribo_trans-likeQ_tRNA_tgttgt_generalQUEUINE TRNA-RIBOSYLTRANSFERASEQUEUINE TRNA-RIBOSYLTRANSFERASETGTtRNA-guanine transglycosylaseQueuine tRNA-ribosyltransferase2.4.2.29Guanine insertion enzymetRNA-guanine transglycosylasetgtSNSL254_A0451Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).Binds 1 zinc ion per subunit.Homodimer. Within each dimer, one monomer is responsible for RNA recognition and catalysis, while the other monomer binds to the replacement base PreQ1.Belongs to the queuine tRNA-ribosyltransferase family.Queuine tRNA-ribosyltransferase425221375RNA binding245251RNA binding; important for wobble base 34 recognition269273Proton acceptor89Nucleophile26493143187214302304307333substrate2008-09-23142522c39369158b815ee4bef23333bb4a6fe2MKFELDTTDGRARRGRLVFDRGVVETPAFMPVGTYGTVKGMTPEEVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSMEMSLRWAKRSRDRFDSLGNKNALFGIIQGSVYEDLRDISVKGLVEIGFDGYAVGGLAVGEPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKHKSDTSPLDAECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKLESFVTEFYQRQGRPVPPLNVDtrue