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B4SWP7

- TGT_SALNS

UniProt

B4SWP7 - TGT_SALNS

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Protein

Queuine tRNA-ribosyltransferase

Gene

tgt

Organism
Salmonella newport (strain SL254)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).UniRule annotation

Catalytic activityi

Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine.UniRule annotation
Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei89 – 891NucleophileUniRule annotation
Binding sitei90 – 901SubstrateUniRule annotation
Metal bindingi302 – 3021ZincUniRule annotation
Metal bindingi304 – 3041ZincUniRule annotation
Metal bindingi307 – 3071ZincUniRule annotation
Metal bindingi333 – 3331ZincUniRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. queuine tRNA-ribosyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. queuosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Queuosine biosynthesis, tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciSENT423368:GHJB-443-MONOMER.
UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
Queuine tRNA-ribosyltransferaseUniRule annotation (EC:2.4.2.29UniRule annotation)
Alternative name(s):
Guanine insertion enzymeUniRule annotation
tRNA-guanine transglycosylaseUniRule annotation
Gene namesi
Name:tgtUniRule annotation
Ordered Locus Names:SNSL254_A0451
OrganismiSalmonella newport (strain SL254)
Taxonomic identifieri423368 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000008824: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 375375Queuine tRNA-ribosyltransferasePRO_1000097562Add
BLAST

Proteomic databases

PRIDEiB4SWP7.

Interactioni

Protein-protein interaction databases

STRINGi423368.SNSL254_A0451.

Structurei

3D structure databases

ProteinModelPortaliB4SWP7.
SMRiB4SWP7. Positions 1-364.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the queuine tRNA-ribosyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0343.
HOGENOMiHOG000223473.
OMAiFGSDIIM.
OrthoDBiEOG6SNDVG.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PANTHERiPTHR11962. PTHR11962. 1 hit.
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.

Sequencei

Sequence statusi: Complete.

B4SWP7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKFELDTTDG RARRGRLVFD RGVVETPAFM PVGTYGTVKG MTPEEVEATG
60 70 80 90 100
AQIILGNTFH LWLRPGQEIM KLHGDLHDFM QWKGPILTDS GGFQVFSLGD
110 120 130 140 150
IRKITEQGVH FRNPINGDPI FLDPEKSMEI QYDLGSDIVM IFDECTPYPA
160 170 180 190 200
DWDYAKRSME MSLRWAKRSR DRFDSLGNKN ALFGIIQGSV YEDLRDISVK
210 220 230 240 250
GLVEIGFDGY AVGGLAVGEP KADMHRILEH VCPQIPADKP RYLMGVGKPE
260 270 280 290 300
DLVEGVRRGI DMFDCVMPTR NARNGHLFVT DGVVKIRNAK HKSDTSPLDA
310 320 330 340 350
ECDCYTCRNY SRAYLHHLDR CNEILGARLN TIHNLRYYQR LMAGLRKAIE
360 370
EGKLESFVTE FYQRQGRPVP PLNVD
Length:375
Mass (Da):42,522
Last modified:September 23, 2008 - v1
Checksum:iC7F7178062F05443
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001113 Genomic DNA. Translation: ACF63460.1.
RefSeqiYP_002039647.1. NC_011080.1.

Genome annotation databases

EnsemblBacteriaiACF63460; ACF63460; SNSL254_A0451.
PATRICi18519336. VBISalEnt114557_0587.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001113 Genomic DNA. Translation: ACF63460.1 .
RefSeqi YP_002039647.1. NC_011080.1.

3D structure databases

ProteinModelPortali B4SWP7.
SMRi B4SWP7. Positions 1-364.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 423368.SNSL254_A0451.

Proteomic databases

PRIDEi B4SWP7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACF63460 ; ACF63460 ; SNSL254_A0451 .
PATRICi 18519336. VBISalEnt114557_0587.

Phylogenomic databases

eggNOGi COG0343.
HOGENOMi HOG000223473.
OMAi FGSDIIM.
OrthoDBi EOG6SNDVG.

Enzyme and pathway databases

UniPathwayi UPA00392 .
BioCyci SENT423368:GHJB-443-MONOMER.

Family and domain databases

Gene3Di 3.20.20.105. 1 hit.
HAMAPi MF_00168. Q_tRNA_Tgt.
InterProi IPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view ]
PANTHERi PTHR11962. PTHR11962. 1 hit.
Pfami PF01702. TGT. 1 hit.
[Graphical view ]
SUPFAMi SSF51713. SSF51713. 1 hit.
TIGRFAMsi TIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Comparative genomics of 28 Salmonella enterica isolates: evidence for CRISPR-mediated adaptive sublineage evolution."
    Fricke W.F., Mammel M.K., McDermott P.F., Tartera C., White D.G., Leclerc J.E., Ravel J., Cebula T.A.
    J. Bacteriol. 193:3556-3568(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SL254.

Entry informationi

Entry nameiTGT_SALNS
AccessioniPrimary (citable) accession number: B4SWP7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: November 26, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3