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B4SWP7

- TGT_SALNS

UniProt

B4SWP7 - TGT_SALNS

Protein

Queuine tRNA-ribosyltransferase

Gene

tgt

Organism
Salmonella newport (strain SL254)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (23 Sep 2008)
      Previous versions | rss
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    Functioni

    Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).UniRule annotation

    Catalytic activityi

    Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine.UniRule annotation
    Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine.UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei89 – 891NucleophileUniRule annotation
    Binding sitei90 – 901SubstrateUniRule annotation
    Metal bindingi302 – 3021ZincUniRule annotation
    Metal bindingi304 – 3041ZincUniRule annotation
    Metal bindingi307 – 3071ZincUniRule annotation
    Metal bindingi333 – 3331ZincUniRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. queuine tRNA-ribosyltransferase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. queuosine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Queuosine biosynthesis, tRNA processing

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciSENT423368:GHJB-443-MONOMER.
    UniPathwayiUPA00392.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Queuine tRNA-ribosyltransferaseUniRule annotation (EC:2.4.2.29UniRule annotation)
    Alternative name(s):
    Guanine insertion enzymeUniRule annotation
    tRNA-guanine transglycosylaseUniRule annotation
    Gene namesi
    Name:tgtUniRule annotation
    Ordered Locus Names:SNSL254_A0451
    OrganismiSalmonella newport (strain SL254)
    Taxonomic identifieri423368 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000008824: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 375375Queuine tRNA-ribosyltransferasePRO_1000097562Add
    BLAST

    Proteomic databases

    PRIDEiB4SWP7.

    Interactioni

    Protein-protein interaction databases

    STRINGi423368.SNSL254_A0451.

    Structurei

    3D structure databases

    ProteinModelPortaliB4SWP7.
    SMRiB4SWP7. Positions 1-364.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the queuine tRNA-ribosyltransferase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0343.
    HOGENOMiHOG000223473.
    OMAiFGSDIIM.
    OrthoDBiEOG6SNDVG.

    Family and domain databases

    Gene3Di3.20.20.105. 1 hit.
    HAMAPiMF_00168. Q_tRNA_Tgt.
    InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
    IPR002616. tRNA_ribo_trans-like.
    [Graphical view]
    PANTHERiPTHR11962. PTHR11962. 1 hit.
    PfamiPF01702. TGT. 1 hit.
    [Graphical view]
    SUPFAMiSSF51713. SSF51713. 1 hit.
    TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
    TIGR00449. tgt_general. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B4SWP7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKFELDTTDG RARRGRLVFD RGVVETPAFM PVGTYGTVKG MTPEEVEATG    50
    AQIILGNTFH LWLRPGQEIM KLHGDLHDFM QWKGPILTDS GGFQVFSLGD 100
    IRKITEQGVH FRNPINGDPI FLDPEKSMEI QYDLGSDIVM IFDECTPYPA 150
    DWDYAKRSME MSLRWAKRSR DRFDSLGNKN ALFGIIQGSV YEDLRDISVK 200
    GLVEIGFDGY AVGGLAVGEP KADMHRILEH VCPQIPADKP RYLMGVGKPE 250
    DLVEGVRRGI DMFDCVMPTR NARNGHLFVT DGVVKIRNAK HKSDTSPLDA 300
    ECDCYTCRNY SRAYLHHLDR CNEILGARLN TIHNLRYYQR LMAGLRKAIE 350
    EGKLESFVTE FYQRQGRPVP PLNVD 375
    Length:375
    Mass (Da):42,522
    Last modified:September 23, 2008 - v1
    Checksum:iC7F7178062F05443
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001113 Genomic DNA. Translation: ACF63460.1.
    RefSeqiYP_002039647.1. NC_011080.1.

    Genome annotation databases

    EnsemblBacteriaiACF63460; ACF63460; SNSL254_A0451.
    PATRICi18519336. VBISalEnt114557_0587.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001113 Genomic DNA. Translation: ACF63460.1 .
    RefSeqi YP_002039647.1. NC_011080.1.

    3D structure databases

    ProteinModelPortali B4SWP7.
    SMRi B4SWP7. Positions 1-364.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 423368.SNSL254_A0451.

    Proteomic databases

    PRIDEi B4SWP7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACF63460 ; ACF63460 ; SNSL254_A0451 .
    PATRICi 18519336. VBISalEnt114557_0587.

    Phylogenomic databases

    eggNOGi COG0343.
    HOGENOMi HOG000223473.
    OMAi FGSDIIM.
    OrthoDBi EOG6SNDVG.

    Enzyme and pathway databases

    UniPathwayi UPA00392 .
    BioCyci SENT423368:GHJB-443-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.105. 1 hit.
    HAMAPi MF_00168. Q_tRNA_Tgt.
    InterProi IPR004803. Queuine_tRNA-ribosylTrfase.
    IPR002616. tRNA_ribo_trans-like.
    [Graphical view ]
    PANTHERi PTHR11962. PTHR11962. 1 hit.
    Pfami PF01702. TGT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51713. SSF51713. 1 hit.
    TIGRFAMsi TIGR00430. Q_tRNA_tgt. 1 hit.
    TIGR00449. tgt_general. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Comparative genomics of 28 Salmonella enterica isolates: evidence for CRISPR-mediated adaptive sublineage evolution."
      Fricke W.F., Mammel M.K., McDermott P.F., Tartera C., White D.G., Leclerc J.E., Ravel J., Cebula T.A.
      J. Bacteriol. 193:3556-3568(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SL254.

    Entry informationi

    Entry nameiTGT_SALNS
    AccessioniPrimary (citable) accession number: B4SWP7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: September 23, 2008
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3