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B4SP18

- SYI_STRM5

UniProt

B4SP18 - SYI_STRM5

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Stenotrophomonas maltophilia (strain R551-3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (23 Sep 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei577 – 5771Aminoacyl-adenylateUniRule annotation
    Binding sitei621 – 6211ATPUniRule annotation
    Metal bindingi906 – 9061ZincUniRule annotation
    Metal bindingi909 – 9091ZincUniRule annotation
    Metal bindingi926 – 9261ZincUniRule annotation
    Metal bindingi929 – 9291ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciSMAL391008:GH1H-1158-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Smal_1125
    OrganismiStenotrophomonas maltophilia (strain R551-3)
    Taxonomic identifieri391008 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeStenotrophomonasStenotrophomonas maltophilia group
    ProteomesiUP000001867: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 943943Isoleucine--tRNA ligasePRO_1000189199Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi391008.Smal_1125.

    Structurei

    3D structure databases

    ProteinModelPortaliB4SP18.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi59 – 6911"HIGH" regionAdd
    BLAST
    Motifi618 – 6225"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B4SP18-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQDYKTTLN LPATEFPMRG DLPKREPGIL ARWEEQGLYQ QLRDNAAGRP    50
    LFVLHDGPPY ANGRIHLGHA VNKILKDIIV KSRYLAGFDA PYVPGWDCHG 100
    LPIEIAVEKK WGKVGTKLDA VEFRQKCREF AEEQINIQRV DFKRLGVTGD 150
    WDNPYKTLSF DFEANEIRAL AKVVANGHLV RGAKPVYWCF DCGSALAEAE 200
    IEYQEKESPA IDVAYAARDA QAIGQAFGVS VPADVEVAVP IWTTTPWTLP 250
    ASLAVSLGAE INYVLAEGPA HNGKRRWLVL AAALAERALQ RYGVEAVVLH 300
    GEADGSALEN QLLAHPFYPE REILVLNGEH VSDEDGTGAV HTAPGHGQED 350
    YVVSQKYGLL DKYNAGQVTP IDGRGVYLES TPPAGDVVLA GQHLWKAQEA 400
    IVGVLRDNGA LLAFHPIRHS YPHCWRHKTP VVFRATPQWF ISMDKANLRN 450
    DALAAIDTVG WFPSWGKARI QSMVDGRPDW TISRQRTWGV PIALFTHRQT 500
    GEIHPRSVEL MQQVADRVEA EGIDVWYSLD AAELLGAEAA DYEKVTDILD 550
    VWFDSGVTHE GVLAARGFGK PADLYLEGSD QHRGWFQSSL LTGVAIDKRA 600
    PYKQCLTHGF TVDEHGRKMS KSLGNGIEPQ DIMNKLGADI LRLWIASADY 650
    SNEMSLSQEI LKRNADAYRR LRNTARFLLG NLDGFDPAQH LQPLDQMVAL 700
    DRWIVHRAWE LQEKIKAAYD GYNMAEIVQL LLNFCSVDLG SLYLDVTKDR 750
    LYTMPTDSSG RRSAQSAMYH IAEAFTRWVA PILTFTADEL WGYLPGERAE 800
    HVLFTTWYDG LAPLPADAQL NAADFDQLLA VREQVAKVLE PMRANGAIGA 850
    ALEAEITIAA SEEQAARWQP LADELRFLFI SGDVQVRPAT TDEVFVSAQP 900
    TTKAKCVRCW HHRADVGSNA DHPELCGRCV SNITGAGEVR SWF 943
    Length:943
    Mass (Da):104,808
    Last modified:September 23, 2008 - v1
    Checksum:i7BD71ACF615A5DD0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001111 Genomic DNA. Translation: ACF50830.1.
    RefSeqiWP_012510415.1. NC_011071.1.
    YP_002027513.1. NC_011071.1.

    Genome annotation databases

    EnsemblBacteriaiACF50830; ACF50830; Smal_1125.
    GeneIDi6474976.
    KEGGismt:Smal_1125.
    PATRICi23707138. VBISteMal40512_1142.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001111 Genomic DNA. Translation: ACF50830.1 .
    RefSeqi WP_012510415.1. NC_011071.1.
    YP_002027513.1. NC_011071.1.

    3D structure databases

    ProteinModelPortali B4SP18.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 391008.Smal_1125.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACF50830 ; ACF50830 ; Smal_1125 .
    GeneIDi 6474976.
    KEGGi smt:Smal_1125.
    PATRICi 23707138. VBISteMal40512_1142.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci SMAL391008:GH1H-1158-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: R551-3.

    Entry informationi

    Entry nameiSYI_STRM5
    AccessioniPrimary (citable) accession number: B4SP18
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: September 23, 2008
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3