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B4SP18

- SYI_STRM5

UniProt

B4SP18 - SYI_STRM5

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Stenotrophomonas maltophilia (strain R551-3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei577 – 5771Aminoacyl-adenylateUniRule annotation
Binding sitei621 – 6211ATPUniRule annotation
Metal bindingi906 – 9061ZincUniRule annotation
Metal bindingi909 – 9091ZincUniRule annotation
Metal bindingi926 – 9261ZincUniRule annotation
Metal bindingi929 – 9291ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSMAL391008:GH1H-1158-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Smal_1125
OrganismiStenotrophomonas maltophilia (strain R551-3)
Taxonomic identifieri391008 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeStenotrophomonasStenotrophomonas maltophilia group
ProteomesiUP000001867: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 943943Isoleucine--tRNA ligasePRO_1000189199Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi391008.Smal_1125.

Structurei

3D structure databases

ProteinModelPortaliB4SP18.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 6911"HIGH" regionAdd
BLAST
Motifi618 – 6225"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4SP18-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQDYKTTLN LPATEFPMRG DLPKREPGIL ARWEEQGLYQ QLRDNAAGRP
60 70 80 90 100
LFVLHDGPPY ANGRIHLGHA VNKILKDIIV KSRYLAGFDA PYVPGWDCHG
110 120 130 140 150
LPIEIAVEKK WGKVGTKLDA VEFRQKCREF AEEQINIQRV DFKRLGVTGD
160 170 180 190 200
WDNPYKTLSF DFEANEIRAL AKVVANGHLV RGAKPVYWCF DCGSALAEAE
210 220 230 240 250
IEYQEKESPA IDVAYAARDA QAIGQAFGVS VPADVEVAVP IWTTTPWTLP
260 270 280 290 300
ASLAVSLGAE INYVLAEGPA HNGKRRWLVL AAALAERALQ RYGVEAVVLH
310 320 330 340 350
GEADGSALEN QLLAHPFYPE REILVLNGEH VSDEDGTGAV HTAPGHGQED
360 370 380 390 400
YVVSQKYGLL DKYNAGQVTP IDGRGVYLES TPPAGDVVLA GQHLWKAQEA
410 420 430 440 450
IVGVLRDNGA LLAFHPIRHS YPHCWRHKTP VVFRATPQWF ISMDKANLRN
460 470 480 490 500
DALAAIDTVG WFPSWGKARI QSMVDGRPDW TISRQRTWGV PIALFTHRQT
510 520 530 540 550
GEIHPRSVEL MQQVADRVEA EGIDVWYSLD AAELLGAEAA DYEKVTDILD
560 570 580 590 600
VWFDSGVTHE GVLAARGFGK PADLYLEGSD QHRGWFQSSL LTGVAIDKRA
610 620 630 640 650
PYKQCLTHGF TVDEHGRKMS KSLGNGIEPQ DIMNKLGADI LRLWIASADY
660 670 680 690 700
SNEMSLSQEI LKRNADAYRR LRNTARFLLG NLDGFDPAQH LQPLDQMVAL
710 720 730 740 750
DRWIVHRAWE LQEKIKAAYD GYNMAEIVQL LLNFCSVDLG SLYLDVTKDR
760 770 780 790 800
LYTMPTDSSG RRSAQSAMYH IAEAFTRWVA PILTFTADEL WGYLPGERAE
810 820 830 840 850
HVLFTTWYDG LAPLPADAQL NAADFDQLLA VREQVAKVLE PMRANGAIGA
860 870 880 890 900
ALEAEITIAA SEEQAARWQP LADELRFLFI SGDVQVRPAT TDEVFVSAQP
910 920 930 940
TTKAKCVRCW HHRADVGSNA DHPELCGRCV SNITGAGEVR SWF
Length:943
Mass (Da):104,808
Last modified:September 23, 2008 - v1
Checksum:i7BD71ACF615A5DD0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001111 Genomic DNA. Translation: ACF50830.1.
RefSeqiYP_002027513.1. NC_011071.1.

Genome annotation databases

EnsemblBacteriaiACF50830; ACF50830; Smal_1125.
GeneIDi6474976.
KEGGismt:Smal_1125.
PATRICi23707138. VBISteMal40512_1142.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001111 Genomic DNA. Translation: ACF50830.1 .
RefSeqi YP_002027513.1. NC_011071.1.

3D structure databases

ProteinModelPortali B4SP18.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 391008.Smal_1125.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACF50830 ; ACF50830 ; Smal_1125 .
GeneIDi 6474976.
KEGGi smt:Smal_1125.
PATRICi 23707138. VBISteMal40512_1142.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SMAL391008:GH1H-1158-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: R551-3.

Entry informationi

Entry nameiSYI_STRM5
AccessioniPrimary (citable) accession number: B4SP18
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 23, 2008
Last modified: October 29, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3