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Protein

Pimeloyl-[acyl-carrier protein] methyl ester esterase

Gene

bioH

Organism
Stenotrophomonas maltophilia (strain R551-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters.UniRule annotation

Catalytic activityi

Pimeloyl-[acyl-carrier protein] methyl ester + H2O = pimeloyl-[acyl-carrier protein] + methanol.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in the pathway biotin biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei18Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1
Active sitei78NucleophileUniRule annotation1
Active sitei203UniRule annotation1
Active sitei231UniRule annotation1
Binding sitei231SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Keywords - Biological processi

Biotin biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00078.

Protein family/group databases

ESTHERistrm5-bioh. BioH.

Names & Taxonomyi

Protein namesi
Recommended name:
Pimeloyl-[acyl-carrier protein] methyl ester esteraseUniRule annotation (EC:3.1.1.85UniRule annotation)
Alternative name(s):
Biotin synthesis protein BioHUniRule annotation
Carboxylesterase BioHUniRule annotation
Gene namesi
Name:bioHUniRule annotation
Ordered Locus Names:Smal_3829
OrganismiStenotrophomonas maltophilia (strain R551-3)
Taxonomic identifieri391008 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeStenotrophomonasStenotrophomonas maltophilia group
Proteomesi
  • UP000001867 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001400061 – 259Pimeloyl-[acyl-carrier protein] methyl ester esteraseAdd BLAST259

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi391008.Smal_3829.

Structurei

3D structure databases

ProteinModelPortaliB4SM83.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni78 – 79Substrate bindingUniRule annotation2
Regioni139 – 143Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D3C. Bacteria.
COG0596. LUCA.
HOGENOMiHOG000028062.
KOiK02170.
OMAiNEFRTHA.
OrthoDBiPOG091H04D0.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01260. Carboxylester. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR010076. BioH.
[Graphical view]
PfamiPF12697. Abhydrolase_6. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR01738. bioH. 1 hit.

Sequencei

Sequence statusi: Complete.

B4SM83-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHIEVTGRGP DLVLIHGWAL QGGVFAPLVQ RLADQFTLHL VDLPGHGHSR
60 70 80 90 100
DDTTPLRLPF VVNAIAAATP PAVWCGWSLG GLFALHAAAT LPKVRGLAMI
110 120 130 140 150
AATPRFVRGE DWPHAVEPAV FEQFGRELAS DFGGTLERFL ALDVMGSAHA
160 170 180 190 200
REELRTLRQR LVERGAPTER ALLEGLRLLE STDLRGALPT LGKPSLWIAG
210 220 230 240 250
QRDRLVSPAA MQAAAALALG GQALTIAHGG HAPFLGHADE VAAALQHFVA

GLSPADGGQ
Length:259
Mass (Da):27,415
Last modified:September 23, 2008 - v1
Checksum:i9DA185290C9BF372
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001111 Genomic DNA. Translation: ACF53528.1.
RefSeqiWP_012512400.1. NC_011071.1.

Genome annotation databases

EnsemblBacteriaiACF53528; ACF53528; Smal_3829.
KEGGismt:Smal_3829.
PATRICi23712704. VBISteMal40512_3879.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001111 Genomic DNA. Translation: ACF53528.1.
RefSeqiWP_012512400.1. NC_011071.1.

3D structure databases

ProteinModelPortaliB4SM83.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi391008.Smal_3829.

Protein family/group databases

ESTHERistrm5-bioh. BioH.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACF53528; ACF53528; Smal_3829.
KEGGismt:Smal_3829.
PATRICi23712704. VBISteMal40512_3879.

Phylogenomic databases

eggNOGiENOG4105D3C. Bacteria.
COG0596. LUCA.
HOGENOMiHOG000028062.
KOiK02170.
OMAiNEFRTHA.
OrthoDBiPOG091H04D0.

Enzyme and pathway databases

UniPathwayiUPA00078.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01260. Carboxylester. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR010076. BioH.
[Graphical view]
PfamiPF12697. Abhydrolase_6. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR01738. bioH. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBIOH_STRM5
AccessioniPrimary (citable) accession number: B4SM83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 23, 2008
Last modified: November 2, 2016
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.