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B4SM81

- BIOB_STRM5

UniProt

B4SM81 - BIOB_STRM5

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Protein

Biotin synthase

Gene
bioB, Smal_3827
Organism
Stenotrophomonas maltophilia (strain R551-3)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi55 – 551Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi59 – 591Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi62 – 621Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi99 – 991Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi130 – 1301Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi190 – 1901Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi262 – 2621Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSMAL391008:GH1H-3906-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Smal_3827
OrganismiStenotrophomonas maltophilia (strain R551-3)
Taxonomic identifieri391008 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeStenotrophomonasStenotrophomonas maltophilia group
ProteomesiUP000001867: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 347347Biotin synthaseUniRule annotationPRO_0000381666Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi391008.Smal_3827.

Structurei

3D structure databases

ProteinModelPortaliB4SM81.
SMRiB4SM81. Positions 6-314.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B4SM81-1 [UniParc]FASTAAdd to Basket

« Hide

MAAAIRHDWQ HDELQALFDL PFPELLFRAA AVHREHFDPA QVQVSTLLSV    50
KTGGCPEDCA YCPQAQRYNT GVNAQKLMET DAVLAKARQA KAAGASRFCM 100
GAAWRSPKDR DIPKVAAMIA GVKALGLETC ATLGMLSGGQ ARALKDAGLD 150
YYNHNLDTAP DYYDSIIHTR QYQDRLDTLE HVRDAGLKTC CGGIVGMGET 200
RAQRVGLLLA LATLPAHPDS VPINKLVQVA GTPLHGSAEL DPFEFVRMIA 250
VARIVMPRSM VRLSAGREAM SDELQALCFV AGANSIFYGD KLLTTGNPES 300
ERDLALFARL GLQPMAVQVD AEGHDHGGTV HADISADAPG CGCAHAA 347
Length:347
Mass (Da):37,186
Last modified:September 23, 2008 - v1
Checksum:i3BE804ABC30CAD17
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001111 Genomic DNA. Translation: ACF53526.1.
RefSeqiWP_006400133.1. NC_011071.1.
YP_002030209.1. NC_011071.1.

Genome annotation databases

EnsemblBacteriaiACF53526; ACF53526; Smal_3827.
GeneIDi6474710.
KEGGismt:Smal_3827.
PATRICi23712700. VBISteMal40512_3877.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001111 Genomic DNA. Translation: ACF53526.1 .
RefSeqi WP_006400133.1. NC_011071.1.
YP_002030209.1. NC_011071.1.

3D structure databases

ProteinModelPortali B4SM81.
SMRi B4SM81. Positions 6-314.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 391008.Smal_3827.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACF53526 ; ACF53526 ; Smal_3827 .
GeneIDi 6474710.
KEGGi smt:Smal_3827.
PATRICi 23712700. VBISteMal40512_3877.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci SMAL391008:GH1H-3906-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: R551-3.

Entry informationi

Entry nameiBIOB_STRM5
AccessioniPrimary (citable) accession number: B4SM81
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 23, 2008
Last modified: September 3, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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