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B4SJ16

- LIPA_STRM5

UniProt

B4SJ16 - LIPA_STRM5

Protein

Lipoyl synthase

Gene

lipA

Organism
Stenotrophomonas maltophilia (strain R551-3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 1 (23 Sep 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi81 – 811Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi86 – 861Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi92 – 921Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi107 – 1071Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi111 – 1111Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi114 – 1141Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciSMAL391008:GH1H-3517-MONOMER.
    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lip-synUniRule annotation
    Short name:
    LSUniRule annotation
    Lipoate synthaseUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Sulfur insertion protein LipAUniRule annotation
    Gene namesi
    Name:lipAUniRule annotation
    Ordered Locus Names:Smal_3442
    OrganismiStenotrophomonas maltophilia (strain R551-3)
    Taxonomic identifieri391008 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeStenotrophomonasStenotrophomonas maltophilia group
    ProteomesiUP000001867: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 336336Lipoyl synthasePRO_1000099638Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi391008.Smal_3442.

    Structurei

    3D structure databases

    ProteinModelPortaliB4SJ16.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0320.
    HOGENOMiHOG000235997.
    KOiK03644.
    OMAiHPHIPTK.
    OrthoDBiEOG6038ZS.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B4SJ16-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTESTARSIP LQIVQGDSPS AAPLQAGVKQ LGGDKINRSP VQFADAPVLR    50
    KPSWIRVRIP SGNAVQNLKA KLRENRLVTV CEEASCPNIH ECFGHGTATF 100
    MILGEVCTRR CSFCDVAHGR PKPPDANEPA SLGQTVADMG LKYVVVTSVD 150
    RDDLRDGGAQ HFVDCISAIR EKSPGTRIEV LTPDFRGKGR MERALEILAQ 200
    NPPDVFNHNI ETVPDLYRNV RPGADYQWSL NLLKNFKAQH PEVPTKSGIM 250
    LGLGEEFEQI KATMRDLRAH DVDMITIGQY LQPTAHHHPV LKYWTPEDYK 300
    ALEDYGYELG FSHVASGPMV RSSYHADVQA KGAGVS 336
    Length:336
    Mass (Da):37,062
    Last modified:September 23, 2008 - v1
    Checksum:i90B3A5ED3A2CFF99
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001111 Genomic DNA. Translation: ACF53141.1.
    RefSeqiWP_006392304.1. NC_011071.1.
    YP_002029824.1. NC_011071.1.

    Genome annotation databases

    EnsemblBacteriaiACF53141; ACF53141; Smal_3442.
    GeneIDi6476721.
    KEGGismt:Smal_3442.
    PATRICi23711888. VBISteMal40512_3475.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001111 Genomic DNA. Translation: ACF53141.1 .
    RefSeqi WP_006392304.1. NC_011071.1.
    YP_002029824.1. NC_011071.1.

    3D structure databases

    ProteinModelPortali B4SJ16.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 391008.Smal_3442.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACF53141 ; ACF53141 ; Smal_3442 .
    GeneIDi 6476721.
    KEGGi smt:Smal_3442.
    PATRICi 23711888. VBISteMal40512_3475.

    Phylogenomic databases

    eggNOGi COG0320.
    HOGENOMi HOG000235997.
    KOi K03644.
    OMAi HPHIPTK.
    OrthoDBi EOG6038ZS.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .
    BioCyci SMAL391008:GH1H-3517-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: R551-3.

    Entry informationi

    Entry nameiLIPA_STRM5
    AccessioniPrimary (citable) accession number: B4SJ16
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: September 23, 2008
    Last modified: October 1, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3