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Protein

Pyrophosphate--fructose 6-phosphate 1-phosphotransferase

Gene

pfp

Organism
Stenotrophomonas maltophilia (strain R551-3)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis. Consistently, PPi-PFK can replace the enzymes of both the forward (ATP-PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.UniRule annotation

Catalytic activityi

Diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Non-allosteric.UniRule annotation

Pathway: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (pfp)
  4. Fructose-bisphosphate aldolase (Smal_3213)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131Diphosphate; via amide nitrogenUniRule annotation
Metal bindingi111 – 1111Magnesium; catalyticUniRule annotation
Sitei112 – 1121Important for catalytic activity and substrate specificity; stabilizes the transition state when the phosphoryl donor is PPi; prevents ATP from binding by mimicking the alpha-phosphate group of ATPUniRule annotation
Sitei138 – 1381Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPiUniRule annotation
Active sitei141 – 1411Proton acceptorUniRule annotation
Binding sitei244 – 2441SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationImported, Transferase

Keywords - Biological processi

GlycolysisUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciSMAL391008:GH1H-3378-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrophosphate--fructose 6-phosphate 1-phosphotransferaseUniRule annotation (EC:2.7.1.90UniRule annotation)
Alternative name(s):
6-phosphofructokinase, pyrophosphate dependentUniRule annotation
PPi-dependent phosphofructokinaseUniRule annotation
Pyrophosphate-dependent 6-phosphofructose-1-kinaseUniRule annotation
Gene namesi
Name:pfpUniRule annotation
Ordered Locus Names:Smal_3303Imported
OrganismiStenotrophomonas maltophilia (strain R551-3)Imported
Taxonomic identifieri391008 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeStenotrophomonasStenotrophomonas maltophilia group
ProteomesiUP000001867 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi391008.Smal_3303.

Structurei

3D structure databases

ProteinModelPortaliB4SI38.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni139 – 1413Substrate bindingUniRule annotation
Regioni187 – 1893Substrate bindingUniRule annotation
Regioni295 – 2984Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Clade "B2" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000007357.
KOiK00850.
OMAiCGVIETA.
OrthoDBiEOG6PP9HS.

Family and domain databases

HAMAPiMF_01978. Phosphofructokinase_II_B2.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR011404. PPi-PFK_XF0274.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF036483. PFK_XF0274. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

B4SI38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRNGTLLYAQ SGGVTAVINA TAAAVIEQAR AKGIKVLAAR NGILGALREE
60 70 80 90 100
LIDTSKESAA AIRALAHTPG GAFGSCRVKL KSLDADRARY DRLLQVLQAH
110 120 130 140 150
DVRWFLYNGG NDSADTALKV SQLAKASGYD LTCIGVPKTI DNDLAVTDTC
160 170 180 190 200
PGFGSAAKYT AVSVREAALD VAAMAETSTR VFIYEAMGRH AGWLAAAAGL
210 220 230 240 250
AGNGDDEAPH IILLPERAYD EAVFLAKVKA VVERVGYCVV VASEGIATAD
260 270 280 290 300
GRFVADAGGG KDSFGHSQLG GVAAHLAGRV KDQLGLKVHW ALPDYLQRSA
310 320 330 340 350
RHLASKTDWE QAQAVGKAAV QLALKGQNGV MPVIVRSSDA PYRWKIEAAP
360 370 380 390 400
LSKIANHEKK MPAGFIRRDG FGITAKARAY LSPLIKGEAP LPYGTDGLPK
410
YVTLKNVAVK QKLPAFEG
Length:418
Mass (Da):44,114
Last modified:September 23, 2008 - v1
Checksum:iB55B8535A4603EBB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001111 Genomic DNA. Translation: ACF53002.1.
RefSeqiWP_006390031.1. NC_011071.1.
YP_002029685.1. NC_011071.1.

Genome annotation databases

EnsemblBacteriaiACF53002; ACF53002; Smal_3303.
KEGGismt:Smal_3303.
PATRICi23711594. VBISteMal40512_3328.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001111 Genomic DNA. Translation: ACF53002.1.
RefSeqiWP_006390031.1. NC_011071.1.
YP_002029685.1. NC_011071.1.

3D structure databases

ProteinModelPortaliB4SI38.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi391008.Smal_3303.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACF53002; ACF53002; Smal_3303.
KEGGismt:Smal_3303.
PATRICi23711594. VBISteMal40512_3328.

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000007357.
KOiK00850.
OMAiCGVIETA.
OrthoDBiEOG6PP9HS.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciSMAL391008:GH1H-3378-MONOMER.

Family and domain databases

HAMAPiMF_01978. Phosphofructokinase_II_B2.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR011404. PPi-PFK_XF0274.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF036483. PFK_XF0274. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: R551-3Imported.

Entry informationi

Entry nameiB4SI38_STRM5
AccessioniPrimary (citable) accession number: B4SI38
Entry historyi
Integrated into UniProtKB/TrEMBL: September 23, 2008
Last sequence update: September 23, 2008
Last modified: May 27, 2015
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.