B4SHI1 (CYOE_STRM5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protoheme IX farnesyltransferase
EC=2.5.1.-

Alternative name(s):
Heme B farnesyltransferase
Heme O synthase

Gene names

Name:	cyoE
Ordered Locus Names:	Smal_0319

Organism

Stenotrophomonas maltophilia (strain R551-3) [Complete proteome] [HAMAP]

Taxonomic identifier

391008 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Stenotrophomonas › Stenotrophomonas maltophilia group › Stenotrophomonas maltophilia

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group By similarity. HAMAP-Rule MF_00154
Pathway	Porphyrin metabolism; heme O biosynthesis; heme O from protoheme: step 1/1. HAMAP-Rule MF_00154
Subcellular location	Cell inner membrane; Multi-pass membrane protein Potential HAMAP-Rule MF_00154.
Miscellaneous	Carbon 2 of the heme B porphyrin ring is defined according to the Fischer nomenclature By similarity. HAMAP-Rule MF_00154
Sequence similarities	Belongs to the UbiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily.

Ontologies

Keywords
Biological process	Heme biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	heme O biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protoheme IX farnesyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	Protoheme IX farnesyltransferase HAMAP-Rule MF_00154		PRO_1000096927
Regions
	Transmembrane	16 – 36	21	Helical; Potential
	Transmembrane	45 – 65	21	Helical; Potential
	Transmembrane	93 – 113	21	Helical; Potential
	Transmembrane	114 – 134	21	Helical; Potential
	Transmembrane	141 – 161	21	Helical; Potential
	Transmembrane	172 – 192	21	Helical; Potential
	Transmembrane	223 – 243	21	Helical; Potential
	Transmembrane	244 – 264	21	Helical; Potential
	Transmembrane	277 – 297	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	B4SHI1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 00C3B34D22A1F334	FASTA	297	33,163
	10 20 30 40 50 60 MFSNYRQYWD LTKPKVVALI VFTALVGMVL AIPGVPSWEQ VRAGVLGFLG IWLAASAAAA 70 80 90 100 110 120 INQLLDAHID AQMARTSWRP LVVGKVKPWQ VLVFASVLIV LSMVILVLWV NLITAVLTFA 130 140 150 160 170 180 SLIGYAVIYT VYLKRATSQN IVIGGLAGAM PPMLGWAAVT GMQGSSDWAY SSLLVLIIFI 190 200 210 220 230 240 WTPPHFWALA IFRREDYAKA EIPMLPVTHG VVHTRKQIMV YSVVLALVCL LPYLVGMSGA 250 260 270 280 290 FYLGGAIVLN AVFLWYAWRM LDPPDELFSM KMFYYSIVYL MALFAFLLVD HWILPWL

References

[1]

"Complete sequence of Stenotrophomonas maltophilia R551-3." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Taghavi S., Monchy S., Newman L., Vangronsveld J., van der Lelie D., Richardson P. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: R551-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001111 Genomic DNA. Translation: ACF50024.1.
RefSeq	YP_002026707.1. NC_011071.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	391008.Smal_0319.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACF50024; ACF50024; Smal_0319.
GeneID	6478115.
KEGG	smt:Smal_0319.
PATRIC	23705456. VBISteMal40512_0320.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0109.
HOGENOM	HOG000237291.
KO	K02301.
OMA	LGILVNW.
ProtClustDB	PRK04375.
Enzyme and pathway databases
BioCyc	SMAL391008:GH1H-331-MONOMER.
UniPathway	UPA00834; UER00712.
Family and domain databases
HAMAP	MF_00154. CyoE_CtaB.
InterPro	IPR006369. Protohaem_IX_farnesylTrfase. IPR000537. UbiA_prenyltransferase. [Graphical view]
Pfam	PF01040. UbiA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01473. cyoE_ctaB. 1 hit.
PROSITE	PS00943. UBIA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYOE_STRM5

Accession

Primary (citable) accession number: B4SHI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	May 1, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program