B4SH00 (B4SH00_PELPB) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Ordered Locus Names:

Ppha_2842

Organism

Pelodictyon phaeoclathratiforme (strain DSM 5477 / BU-1) [Complete proteome] [HAMAP]

Taxonomic identifier

324925 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Pelodictyon

Protein attributes

Sequence length	798 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 RuleBase RU000518 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000518 SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B4SH00 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 1B91AC776D1B57AD	FASTA	798	89,281
	10 20 30 40 50 60 MASKPSTPSA RNRTLIVVES PSKAKTINKY LGDKYTVFAS VGHIKDLPKK EIGLDFDHHY 70 80 90 100 110 120 EPRYEIIPGK EKVVHQLKKL AAEADSILIA TDPDREGEAI AWHISKEIGV TSKPVSRVLF 130 140 150 160 170 180 NEITKTAIIA AIQAPRQIDD RLVRSQQTRQ GLDKIVGYKI SPFLWNVVLR GLSAGRVQSV 190 200 210 220 230 240 ALRLICEREA EISRFQIQEY WSIAAEFVTA GKESFKARLI RFEGDKPEIA NQKQAEALAA 250 260 270 280 290 300 LIRKGNFAVR EIAPRVQQRK QPLPFTTSLL QQAASNQLGF GSKKTMGVAQ QLYEGIDLGK 310 320 330 340 350 360 EGTTGLITYM RTDSTRIGEE AIAEARSYIN QQFGKEYIGF GGAAKPGKNA QDAHEAIRPT 370 380 390 400 410 420 SIFNKPDQVK PYLSADQFKL YELIWKRFLA SMMSAAKIEQ TRVDVEDSAS KFLFRATGSR 430 440 450 460 470 480 VLFPGFMRVY DDQKELDYEA QTSTKEEVEK ELTVKLPERL AVNEPLALDD LDQKQSFTRP 490 500 510 520 530 540 PARYSEATLV KDLDHFGIGR PSTYASIFST LQDRRYVELE KKKIVPTELG KDVSLILVAN 550 560 570 580 590 600 FPDLFNVDFT AFMENELDKV ASGEDEYEKV LDSFYKPLEA VLHLRKSNPI VPQNSEPVTC 610 620 630 640 650 660 EKCGEGLMVV KWTANGKFFG CSCYPKCKNI KAISNNKAKP VDSAILCPSC KEGHMLLRSG 670 680 690 700 710 720 RFGTFLACSG YPKCNTLLNL NKQRHIEPMK TPPVLTTLLC PKCGSPLYLR SGKRGLWLGC 730 740 750 760 770 780 SKFPKCKGRL AWSTLDEKAQ HHWEAIMAEH QKAHPTVQLT MIDGKAVPMT IPVDEIILKA 790 EEAGLITSPS EEIVEITP

References

[1]

"Complete sequence of Pelodictyon phaeoclathratiforme BU-1." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Liu Z., Li T., Zhao F. , Overmann J., Bryant D.A., Richardson P. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 5477 / BU-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001110 Genomic DNA. Translation: ACF44988.1.
RefSeq	YP_002019605.1. NC_011060.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B4SH00.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6461173.
GenomeReviews	Gene locus Ppha_2842 in contig CP001110_GR.
KEGG	pph:Ppha_2842.
PATRIC	22906547. VBIPelPha134556_3029.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG512935.
OMA	EEYWSID.
ProtClustDB	PRK06599.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 3 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B4SH00_PELPB

Accession

Primary (citable) accession number: B4SH00

Entry history

Integrated into UniProtKB/TrEMBL:	September 23, 2008
Last sequence update:	September 23, 2008
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)