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B4SGZ7 (BIOB_PELPB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Ppha_2839
OrganismPelodictyon phaeoclathratiforme (strain DSM 5477 / BU-1) [Complete proteome] [HAMAP]
Taxonomic identifier324925 [NCBI]
Taxonomic lineageBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupPelodictyon

Protein attributes

Sequence length337 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 337337Biotin synthase HAMAP-Rule MF_01694
PRO_0000381521

Sites

Metal binding761Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding801Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding831Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1551Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2161Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2861Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B4SGZ7 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: 77C6DD5DCA77A329

FASTA33736,779
        10         20         30         40         50         60 
MDYRTLHPQI AAAYRVLETG SPISQAEALL LAELPGELVL DLASLANKVK NRYGTGSAGS 

        70         80         90        100        110        120 
ELLHACSIMN AKSGVCGENC RFCAQSRHNR ADIEVYDLVD AESVLIEARN CFAEGVSHFG 

       130        140        150        160        170        180 
IVTSGYGYKR INPEFQRVLD MIDRLHEELP ELSVCASLGM LGEEPAEALA AHGIAHYNIN 

       190        200        210        220        230        240 
IQVVPDRYHD LIADTHSVEE RIETIKLLRK NNISVCCGGI MGVGETMQER IAMIFALQEL 

       250        260        270        280        290        300 
DVSVIPLNVL VPIEGTPLAG KEPLSVAEIV KTFAICRLAH PHKIIKFAAG RETIMKDFQG 

       310        320        330 
LLMLSGANGF LTGGYLTTRG RDIADDRRFA SQIASFN 

« Hide

References

[1]"Complete sequence of Pelodictyon phaeoclathratiforme BU-1."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Liu Z., Li T., Zhao F. expand/collapse author list , Overmann J., Bryant D.A., Richardson P.
Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 5477 / BU-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001110 Genomic DNA. Translation: ACF44985.1.
RefSeqYP_002019602.1. NC_011060.1.

3D structure databases

ProteinModelPortalB4SGZ7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING324925.Ppha_2839.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACF44985; ACF44985; Ppha_2839.
GeneID6461119.
KEGGpph:Ppha_2839.
PATRIC22906539. VBIPelPha134556_3025.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMARDFHGMA.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycPPHA324925:GHBF-2892-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_PELPB
AccessionPrimary (citable) accession number: B4SGZ7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 23, 2008
Last modified: May 14, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways