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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Pelodictyon phaeoclathratiforme (strain DSM 5477 / BU-1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei579 – 5791ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPPHA324925:GHBF-679-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Ppha_0665
OrganismiPelodictyon phaeoclathratiforme (strain DSM 5477 / BU-1)
Taxonomic identifieri324925 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupPelodictyon
ProteomesiUP000002724: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 807807Leucine--tRNA ligasePRO_1000091341Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi324925.Ppha_0665.

Structurei

3D structure databases

ProteinModelPortaliB4SDX3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5112"HIGH" regionAdd
BLAST
Motifi576 – 5805"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

B4SDX3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRYEFSKTEA KWQAWWKEQN TFKTGDDADK PKYYVLDMFP YPSGTGLHVG
60 70 80 90 100
HLEGYTASDI TARYKRSCGY NVLHPMGWDA FGLPAEQFAI KTGTHPKTTT
110 120 130 140 150
EENIRNFKGT LQAMGFSYDW SREINTTDPH YFKWTQWIFL KLYERGLAYM
160 170 180 190 200
SEVDVNWCEE LKTVLANEEV DEKIADGYTV VRRPLRQWVL KITAYADRLL
210 220 230 240 250
ADLDELNWPE NVKQMQRNWI GRSEGVEIDF ELRCHNKKLR VYTTRPDTLF
260 270 280 290 300
GATYLVISPE HPLAEKLATA QQLVEVKNYI SKAKLKTELE RTGLQKEKTG
310 320 330 340 350
VFTGSYAINP ATGEPLPVWI SDFVLISYGT GAIMSVPAHD SRDWEFAKQY
360 370 380 390 400
NLPIIEVIQS PHDVQERVFE EKESICVNSA NNEITLNGLP FCEAFERMAS
410 420 430 440 450
WLETKKAGER KVNYKLRDWI FSRQRYWGEP IPIKHYHDGT LRLETALPLV
460 470 480 490 500
LPEVEAYHPS STGESPLANI PHWLYGSDEH GSFRRETNTM PQWAGSCWYY
510 520 530 540 550
LRFIDPENQE KLVDPEKERY WMNVDLYIGG AEHAVLHLLY ARFWHKVLFD
560 570 580 590 600
LGVVSSREPF KKLFNQGMIL GEDNEKMSKS RGNVIPADHV LERYGADAVR
610 620 630 640 650
LYEMFLGPLE QVKPWNTNGI EGISRFLSKV WRLVYGEHEE SATLLSEEAM
660 670 680 690 700
PEELLRRMHK SIKKVGEDTE QLKFNTAISE MMVFVNELQK SGCKNRSAIE
710 720 730 740 750
TLLVLLSPYA PHITEELWES IGHNYSISQA PFPSFDPKLV EESILTIAVQ
760 770 780 790 800
INGKLRGTFL APAKSPKELL LQEAKKTESV FKFLDGQTIL REIVVVDKLV

NFVVKKE
Length:807
Mass (Da):93,181
Last modified:September 23, 2008 - v1
Checksum:i37E6878C5B71A15B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001110 Genomic DNA. Translation: ACF42964.1.
RefSeqiWP_012507459.1. NC_011060.1.
YP_002017581.1. NC_011060.1.

Genome annotation databases

EnsemblBacteriaiACF42964; ACF42964; Ppha_0665.
GeneIDi6461585.
KEGGipph:Ppha_0665.
PATRICi22901764. VBIPelPha134556_0674.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001110 Genomic DNA. Translation: ACF42964.1.
RefSeqiWP_012507459.1. NC_011060.1.
YP_002017581.1. NC_011060.1.

3D structure databases

ProteinModelPortaliB4SDX3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi324925.Ppha_0665.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACF42964; ACF42964; Ppha_0665.
GeneIDi6461585.
KEGGipph:Ppha_0665.
PATRICi22901764. VBIPelPha134556_0674.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciPPHA324925:GHBF-679-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Pelodictyon phaeoclathratiforme BU-1."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Liu Z., Li T., Zhao F.
    , Overmann J., Bryant D.A., Richardson P.
    Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 5477 / BU-1.

Entry informationi

Entry nameiSYL_PELPB
AccessioniPrimary (citable) accession number: B4SDX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: March 4, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.