Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B4SDX3 (SYL_PELPB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Ppha_0665
OrganismPelodictyon phaeoclathratiforme (strain DSM 5477 / BU-1) [Complete proteome] [HAMAP]
Taxonomic identifier324925 [NCBI]
Taxonomic lineageBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupPelodictyon

Protein attributes

Sequence length807 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 807807Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091341

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif576 – 5805"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5791ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B4SDX3 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: 37E6878C5B71A15B

FASTA80793,181
        10         20         30         40         50         60 
MRYEFSKTEA KWQAWWKEQN TFKTGDDADK PKYYVLDMFP YPSGTGLHVG HLEGYTASDI 

        70         80         90        100        110        120 
TARYKRSCGY NVLHPMGWDA FGLPAEQFAI KTGTHPKTTT EENIRNFKGT LQAMGFSYDW 

       130        140        150        160        170        180 
SREINTTDPH YFKWTQWIFL KLYERGLAYM SEVDVNWCEE LKTVLANEEV DEKIADGYTV 

       190        200        210        220        230        240 
VRRPLRQWVL KITAYADRLL ADLDELNWPE NVKQMQRNWI GRSEGVEIDF ELRCHNKKLR 

       250        260        270        280        290        300 
VYTTRPDTLF GATYLVISPE HPLAEKLATA QQLVEVKNYI SKAKLKTELE RTGLQKEKTG 

       310        320        330        340        350        360 
VFTGSYAINP ATGEPLPVWI SDFVLISYGT GAIMSVPAHD SRDWEFAKQY NLPIIEVIQS 

       370        380        390        400        410        420 
PHDVQERVFE EKESICVNSA NNEITLNGLP FCEAFERMAS WLETKKAGER KVNYKLRDWI 

       430        440        450        460        470        480 
FSRQRYWGEP IPIKHYHDGT LRLETALPLV LPEVEAYHPS STGESPLANI PHWLYGSDEH 

       490        500        510        520        530        540 
GSFRRETNTM PQWAGSCWYY LRFIDPENQE KLVDPEKERY WMNVDLYIGG AEHAVLHLLY 

       550        560        570        580        590        600 
ARFWHKVLFD LGVVSSREPF KKLFNQGMIL GEDNEKMSKS RGNVIPADHV LERYGADAVR 

       610        620        630        640        650        660 
LYEMFLGPLE QVKPWNTNGI EGISRFLSKV WRLVYGEHEE SATLLSEEAM PEELLRRMHK 

       670        680        690        700        710        720 
SIKKVGEDTE QLKFNTAISE MMVFVNELQK SGCKNRSAIE TLLVLLSPYA PHITEELWES 

       730        740        750        760        770        780 
IGHNYSISQA PFPSFDPKLV EESILTIAVQ INGKLRGTFL APAKSPKELL LQEAKKTESV 

       790        800 
FKFLDGQTIL REIVVVDKLV NFVVKKE 

« Hide

References

[1]"Complete sequence of Pelodictyon phaeoclathratiforme BU-1."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Liu Z., Li T., Zhao F. expand/collapse author list , Overmann J., Bryant D.A., Richardson P.
Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 5477 / BU-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001110 Genomic DNA. Translation: ACF42964.1.
RefSeqYP_002017581.1. NC_011060.1.

3D structure databases

ProteinModelPortalB4SDX3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING324925.Ppha_0665.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACF42964; ACF42964; Ppha_0665.
GeneID6461585.
KEGGpph:Ppha_0665.
PATRIC22901764. VBIPelPha134556_0674.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAERYWMNV.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycPPHA324925:GHBF-679-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYL_PELPB
AccessionPrimary (citable) accession number: B4SDX3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: May 14, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries