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B4SDX2

- B4SDX2_PELPB

UniProt

B4SDX2 - B4SDX2_PELPB

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Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Pelodictyon phaeoclathratiforme (strain DSM 5477 / BU-1)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei332 – 3321Coenzyme AUniRule annotation
Binding sitei356 – 3561Coenzyme AUniRule annotation
Binding sitei521 – 5211ATPUniRule annotation
Binding sitei536 – 5361ATPUniRule annotation
Binding sitei544 – 5441Coenzyme A; via carbonyl oxygenUniRule annotation
Binding sitei547 – 5471ATPUniRule annotation
Metal bindingi558 – 5581Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi560 – 5601Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi563 – 5631Magnesium; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi408 – 4103ATPUniRule annotation
Nucleotide bindingi432 – 4376ATPUniRule annotation

GO - Molecular functioni

  1. acetate-CoA ligase activity Source: UniProtKB-HAMAP
  2. AMP binding Source: InterPro
  3. ATP binding Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. acetyl-CoA biosynthetic process from acetate Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciPPHA324925:GHBF-678-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
Short name:
AcCoA synthetaseUniRule annotation
Short name:
AcsUniRule annotation
Alternative name(s):
Acetate--CoA ligaseUniRule annotation
Acyl-activating enzymeUniRule annotation
Gene namesi
Name:acsAUniRule annotation
Ordered Locus Names:Ppha_0664Imported
OrganismiPelodictyon phaeoclathratiforme (strain DSM 5477 / BU-1)Imported
Taxonomic identifieri324925 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupPelodictyon
ProteomesiUP000002724: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei630 – 6301N6-acetyllysineUniRule annotation

Post-translational modificationi

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

Keywords - PTMi

AcetylationUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi324925.Ppha_0664.

Structurei

3D structure databases

ProteinModelPortaliB4SDX2.
SMRiB4SDX2. Positions 32-665.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni214 – 2174Coenzyme A bindingUniRule annotation

Sequence similaritiesi

Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0365.
HOGENOMiHOG000229981.
KOiK01895.
OMAiVEWMDSE.
OrthoDBiEOG68WR2H.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth.
InterProiIPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4SDX2 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATEETTSNA QHTETSSSGE ESISSVLSEK RKFPPSAEFS NNAHISSMEE
60 70 80 90 100
YEKLYAAAEA DPDAYWGGIA EQFHWFKKWD SVLEWNTPYA KWFNGGKTNI
110 120 130 140 150
CYNALDVHVN SWRKNKAAII WEGEEGNQRV LTYGELHRQV SKFANVLKIA
160 170 180 190 200
GIKPGDRVAI YMGMVPELVI AVLACARVGA VHNVIFAGFS AHAITERVND
210 220 230 240 250
SRAKMVICAD GTRRRGGSIN LKNIVDEAIV NTPSIRSVIV LKVTDEEIHM
260 270 280 290 300
HNGMDHWWHD LMGLAADESE PAELDAEHPL FVLYTSGSTG KPKGILHTTA
310 320 330 340 350
GYMVHAASSF KYVFDIKDED IYFCTADVGW ITGHTYMIYG PMLNGATILM
360 370 380 390 400
YEGAPNYPQW DRFWDIINRH KVTIFYTAPT AIRAFIRAGN EWVTKHNLSS
410 420 430 440 450
LRLLGTVGEP INPEAWMWYQ KVVGQEKCPI VDTWWQTETG GIMVSPLPGA
460 470 480 490 500
TPTKPGTATR PLPGIMVDVV RKDGTPCEAN EGGYLVIKKP WPSMLRTIYG
510 520 530 540 550
DNERYEKTYW SEFHDMYFTG DGARKDEDGY IWIMGRVDDV VNVSGHRLGT
560 570 580 590 600
SEVESALVSH EAVAEAAVVS RPDELKGNAL VAFVTLKDEY VGDMTLREAL
610 620 630 640 650
RNHVAKEIGP IAKPDEIRWA KGLPKTRSGK IMRRLLRELA TSNEIKGDVT
660
TLEDFGVLEN LRDQEND
Length:667
Mass (Da):74,643
Last modified:September 23, 2008 - v1
Checksum:i6D0D7BD4DE17DE57
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001110 Genomic DNA. Translation: ACF42963.1.
RefSeqiYP_002017580.1. NC_011060.1.

Genome annotation databases

EnsemblBacteriaiACF42963; ACF42963; Ppha_0664.
GeneIDi6461587.
KEGGipph:Ppha_0664.
PATRICi22901760. VBIPelPha134556_0672.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001110 Genomic DNA. Translation: ACF42963.1 .
RefSeqi YP_002017580.1. NC_011060.1.

3D structure databases

ProteinModelPortali B4SDX2.
SMRi B4SDX2. Positions 32-665.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 324925.Ppha_0664.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACF42963 ; ACF42963 ; Ppha_0664 .
GeneIDi 6461587.
KEGGi pph:Ppha_0664.
PATRICi 22901760. VBIPelPha134556_0672.

Phylogenomic databases

eggNOGi COG0365.
HOGENOMi HOG000229981.
KOi K01895.
OMAi VEWMDSE.
OrthoDBi EOG68WR2H.

Enzyme and pathway databases

BioCyci PPHA324925:GHBF-678-MONOMER.

Family and domain databases

HAMAPi MF_01123. Ac_CoA_synth.
InterProi IPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Pelodictyon phaeoclathratiforme BU-1."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Liu Z., Li T., Zhao F.
    , Overmann J., Bryant D.A., Richardson P.
    Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 5477 / BU-1Imported.

Entry informationi

Entry nameiB4SDX2_PELPB
AccessioniPrimary (citable) accession number: B4SDX2
Entry historyi
Integrated into UniProtKB/TrEMBL: September 23, 2008
Last sequence update: September 23, 2008
Last modified: October 29, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3