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B4SDX2

- B4SDX2_PELPB

UniProt

B4SDX2 - B4SDX2_PELPB

Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Pelodictyon phaeoclathratiforme (strain DSM 5477 / BU-1)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 41 (01 Oct 2014)
      Sequence version 1 (23 Sep 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

    Catalytic activityi

    ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei332 – 3321Coenzyme AUniRule annotation
    Binding sitei356 – 3561Coenzyme AUniRule annotation
    Binding sitei408 – 4081Substrate; via nitrogen amideUniRule annotation
    Binding sitei521 – 5211SubstrateUniRule annotation
    Binding sitei536 – 5361SubstrateUniRule annotation
    Active sitei538 – 5381UniRule annotation
    Binding sitei544 – 5441Coenzyme AUniRule annotation
    Binding sitei547 – 5471SubstrateUniRule annotation
    Metal bindingi558 – 5581Magnesium; via carbonyl oxygenUniRule annotation
    Metal bindingi560 – 5601Magnesium; via carbonyl oxygenUniRule annotation
    Metal bindingi563 – 5631Magnesium; via carbonyl oxygenUniRule annotation
    Binding sitei605 – 6051Coenzyme AUniRule annotation

    GO - Molecular functioni

    1. acetate-CoA ligase activity Source: UniProtKB-HAMAP
    2. AMP binding Source: InterPro
    3. ATP binding Source: UniProtKB-KW
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. acetyl-CoA biosynthetic process from acetate Source: InterPro

    Keywords - Molecular functioni

    LigaseUniRule annotationImported

    Keywords - Ligandi

    ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciPPHA324925:GHBF-678-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
    Short name:
    AcCoA synthetaseUniRule annotation
    Short name:
    AcsUniRule annotation
    Alternative name(s):
    Acetate--CoA ligaseUniRule annotation
    Acyl-activating enzymeUniRule annotation
    Gene namesi
    Name:acsAUniRule annotation
    Ordered Locus Names:Ppha_0664Imported
    OrganismiPelodictyon phaeoclathratiforme (strain DSM 5477 / BU-1)Imported
    Taxonomic identifieri324925 [NCBI]
    Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupPelodictyon
    ProteomesiUP000002724: Chromosome

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei630 – 6301N6-acetyllysineUniRule annotation

    Post-translational modificationi

    Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

    Keywords - PTMi

    AcetylationUniRule annotation

    Interactioni

    Protein-protein interaction databases

    STRINGi324925.Ppha_0664.

    Structurei

    3D structure databases

    ProteinModelPortaliB4SDX2.
    SMRiB4SDX2. Positions 32-665.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni432 – 4376Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0365.
    HOGENOMiHOG000229981.
    KOiK01895.
    OMAiVEWMDSE.
    OrthoDBiEOG68WR2H.

    Family and domain databases

    HAMAPiMF_01123. Ac_CoA_synth.
    InterProiIPR011904. Ac_CoA_lig.
    IPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
    PROSITEiPS00455. AMP_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B4SDX2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATEETTSNA QHTETSSSGE ESISSVLSEK RKFPPSAEFS NNAHISSMEE    50
    YEKLYAAAEA DPDAYWGGIA EQFHWFKKWD SVLEWNTPYA KWFNGGKTNI 100
    CYNALDVHVN SWRKNKAAII WEGEEGNQRV LTYGELHRQV SKFANVLKIA 150
    GIKPGDRVAI YMGMVPELVI AVLACARVGA VHNVIFAGFS AHAITERVND 200
    SRAKMVICAD GTRRRGGSIN LKNIVDEAIV NTPSIRSVIV LKVTDEEIHM 250
    HNGMDHWWHD LMGLAADESE PAELDAEHPL FVLYTSGSTG KPKGILHTTA 300
    GYMVHAASSF KYVFDIKDED IYFCTADVGW ITGHTYMIYG PMLNGATILM 350
    YEGAPNYPQW DRFWDIINRH KVTIFYTAPT AIRAFIRAGN EWVTKHNLSS 400
    LRLLGTVGEP INPEAWMWYQ KVVGQEKCPI VDTWWQTETG GIMVSPLPGA 450
    TPTKPGTATR PLPGIMVDVV RKDGTPCEAN EGGYLVIKKP WPSMLRTIYG 500
    DNERYEKTYW SEFHDMYFTG DGARKDEDGY IWIMGRVDDV VNVSGHRLGT 550
    SEVESALVSH EAVAEAAVVS RPDELKGNAL VAFVTLKDEY VGDMTLREAL 600
    RNHVAKEIGP IAKPDEIRWA KGLPKTRSGK IMRRLLRELA TSNEIKGDVT 650
    TLEDFGVLEN LRDQEND 667
    Length:667
    Mass (Da):74,643
    Last modified:September 23, 2008 - v1
    Checksum:i6D0D7BD4DE17DE57
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001110 Genomic DNA. Translation: ACF42963.1.
    RefSeqiWP_012507458.1. NC_011060.1.
    YP_002017580.1. NC_011060.1.

    Genome annotation databases

    EnsemblBacteriaiACF42963; ACF42963; Ppha_0664.
    GeneIDi6461587.
    KEGGipph:Ppha_0664.
    PATRICi22901760. VBIPelPha134556_0672.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001110 Genomic DNA. Translation: ACF42963.1 .
    RefSeqi WP_012507458.1. NC_011060.1.
    YP_002017580.1. NC_011060.1.

    3D structure databases

    ProteinModelPortali B4SDX2.
    SMRi B4SDX2. Positions 32-665.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 324925.Ppha_0664.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACF42963 ; ACF42963 ; Ppha_0664 .
    GeneIDi 6461587.
    KEGGi pph:Ppha_0664.
    PATRICi 22901760. VBIPelPha134556_0672.

    Phylogenomic databases

    eggNOGi COG0365.
    HOGENOMi HOG000229981.
    KOi K01895.
    OMAi VEWMDSE.
    OrthoDBi EOG68WR2H.

    Enzyme and pathway databases

    BioCyci PPHA324925:GHBF-678-MONOMER.

    Family and domain databases

    HAMAPi MF_01123. Ac_CoA_synth.
    InterProi IPR011904. Ac_CoA_lig.
    IPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
    PROSITEi PS00455. AMP_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Pelodictyon phaeoclathratiforme BU-1."
      US DOE Joint Genome Institute
      Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Liu Z., Li T., Zhao F.
      , Overmann J., Bryant D.A., Richardson P.
      Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 5477 / BU-1Imported.

    Entry informationi

    Entry nameiB4SDX2_PELPB
    AccessioniPrimary (citable) accession number: B4SDX2
    Entry historyi
    Integrated into UniProtKB/TrEMBL: September 23, 2008
    Last sequence update: September 23, 2008
    Last modified: October 1, 2014
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3