B4SCV6 (SYE_PELPB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	Ppha_0464

Organism

Pelodictyon phaeoclathratiforme (strain DSM 5477 / BU-1) [Complete proteome] [HAMAP]

Taxonomic identifier

324925 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Pelodictyon

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000367731
Regions
	Motif	12 – 22	11	"HIGH" region HAMAP MF_00022_B
	Motif	259 – 263	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	262	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4SCV6 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: A8BB1BF060A7CB84	FASTA	502	57,811
	10 20 30 40 50 60 MVGQRVRTRF APSPTGYLHV GGLRTALYNY LFAKKMHGDF IIRIEDTDQT RKVEGAQQNL 70 80 90 100 110 120 IKALEWAGIV ADESPEKGGN YGPYVQSERL DLYGRYCQQL LDDQHAYYCF ATHEELEENR 130 140 150 160 170 180 QLQIKQGLQP KYNRKWLPEE MGGSMPPSVI RKKLDEGGPY VIRMKVPDYV SVWFEDIIRG 190 200 210 220 230 240 PVEFDSATID DQVLMKSDGF PTYHFASVID DHLMEFTHII RGEEWLSSMP KHLLLYEFFG 250 260 270 280 290 300 WEPPKVAHLP LLLNADRSKL SKRQGDVAVE DYIRKGYSND AIINFVAMLG WNEGEGSEQE 310 320 330 340 350 360 VYSMEQLIEK FSLERVGKAG AVFNIDKLNW LEKQYIKNRP TEQLVPVIKP ILLAELERKE 370 380 390 400 410 420 TMMPVDLITS EAYLEKVIEL MRERVGFEHE FVTFSSYFFF EPDSYDEEAV KKRWAPDTNS 430 440 450 460 470 480 LLQEFTEILA TLEEFSAENI EAHLKEFVAP KGLKAAALIH PLRIVSSGVS FGPSLYHMLE 490 500 VLGKETVLRR ILKGREKITF SA

References

[1]

"Complete sequence of Pelodictyon phaeoclathratiforme BU-1." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Liu Z., Li T., Zhao F. , Overmann J., Bryant D.A., Richardson P. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 5477 / BU-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001110 Genomic DNA. Translation: ACF42790.1.
RefSeq	YP_002017407.1. NC_011060.1.
3D structure databases
ProteinModelPortal	B4SCV6.
ModBase	Search...
Protein-protein interaction databases
STRING	B4SCV6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6462145.
GenomeReviews	Gene locus Ppha_0464 in contig CP001110_GR.
KEGG	pph:Ppha_0464.
PATRIC	22901354. VBIPelPha134556_0470.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	MAHIPLI.
ProtClustDB	PRK01406.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_PELPB

Accession

Primary (citable) accession number: B4SCV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program