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B4SAI2 (DDL_PELPB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Ppha_1631
OrganismPelodictyon phaeoclathratiforme (strain DSM 5477 / BU-1) [Complete proteome] [HAMAP]
Taxonomic identifier324925 [NCBI]
Taxonomic lineageBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupPelodictyon

Protein attributes

Sequence length360 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 360360D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000091200

Regions

Domain146 – 352207ATP-grasp
Nucleotide binding179 – 23456ATP By similarity

Sites

Metal binding3051Magnesium or manganese 1 By similarity
Metal binding3191Magnesium or manganese 1 By similarity
Metal binding3191Magnesium or manganese 2 By similarity
Metal binding3211Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
B4SAI2 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: C56688E968C46B78

FASTA36039,470
        10         20         30         40         50         60 
MPRITVALIF GGRSTEHEIS IISAKSIAAN ISAERYKVIP LYITHQGTWL CEGIARDILN 

        70         80         90        100        110        120 
LDLSALLRNS SPEAAAMALD RMVEEAEQKP FNLDFRAIGI EVAFLTLHGT YGEDGRIQGF 

       130        140        150        160        170        180 
LDTCNIPYTG CGVLASALTM DKALTKLCVA DAGIAVAPSI TLLSAEYHAD TEKVHSRIEE 

       190        200        210        220        230        240 
KFIYPFFVKP ANLGSSIGIS KVHHREQLPA ALKSACSLDS KIVVEKAITG REIEVAVLGN 

       250        260        270        280        290        300 
DEPEVSVCGE IEPGSDFYDY HDKYIHNSAK LFIPARIPDE MQSEVRSIAL KAYKALGCRG 

       310        320        330        340        350        360 
MSRIDFFVDE KRGTIVLNEV NTIPGFTDIS MFPRLMAASG HSFPELAERL LQLALETLRP 

« Hide

References

[1]"Complete sequence of Pelodictyon phaeoclathratiforme BU-1."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Liu Z., Li T., Zhao F. expand/collapse author list , Overmann J., Bryant D.A., Richardson P.
Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 5477 / BU-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001110 Genomic DNA. Translation: ACF43868.1.
RefSeqYP_002018485.1. NC_011060.1.

3D structure databases

ProteinModelPortalB4SAI2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING324925.Ppha_1631.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACF43868; ACF43868; Ppha_1631.
GeneID6463210.
KEGGpph:Ppha_1631.
PATRIC22903886. VBIPelPha134556_1718.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAMDKIAMK.
OrthoDBEOG64BQ73.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycPPHA324925:GHBF-1662-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_PELPB
AccessionPrimary (citable) accession number: B4SAI2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: February 19, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways