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Protein

Nitrogenase iron protein

Gene

nifH

Organism
Prosthecochloris aestuarii (strain DSM 271 / SK 413)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein.UniRule annotation

Catalytic activityi

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per dimer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi94Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation1
Metal bindingi131Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 15ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processNitrogen fixation
Ligand4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPAES290512:G1GCD-1657-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogenase iron proteinUniRule annotation (EC:1.18.6.1UniRule annotation)
Alternative name(s):
Nitrogenase Fe proteinUniRule annotation
Nitrogenase component IIUniRule annotation
Nitrogenase reductaseUniRule annotation
Gene namesi
Name:nifHUniRule annotation
Ordered Locus Names:Paes_1627
OrganismiProsthecochloris aestuarii (strain DSM 271 / SK 413)
Taxonomic identifieri290512 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeProsthecochloris
Proteomesi
  • UP000002725 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002118801 – 274Nitrogenase iron proteinAdd BLAST274

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei97ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferaseUniRule annotation1

Post-translational modificationi

The reversible ADP-ribosylation of Arg-97 inactivates the nitrogenase reductase and regulates nitrogenase activity.UniRule annotation

Keywords - PTMi

ADP-ribosylation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi290512.Paes_1627

Structurei

3D structure databases

ProteinModelPortaliB4S9H5
SMRiB4S9H5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NifH/BchL/ChlL family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DSM Bacteria
COG1348 LUCA
HOGENOMiHOG000228826
KOiK02588
OMAiPGDIVCG
OrthoDBiPOG091H0230

Family and domain databases

CDDicd02040 NifH, 1 hit
HAMAPiMF_00533 NifH, 1 hit
InterProiView protein in InterPro
IPR030655 NifH/chlL_CS
IPR000392 NifH/frxC
IPR005977 Nitrogenase_Fe_NifH
IPR027417 P-loop_NTPase
PANTHERiPTHR42864 PTHR42864, 1 hit
PfamiView protein in Pfam
PF00142 Fer4_NifH, 1 hit
PIRSFiPIRSF000363 Nitrogenase_iron, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01287 nifH, 1 hit
PROSITEiView protein in PROSITE
PS00746 NIFH_FRXC_1, 1 hit
PS00692 NIFH_FRXC_2, 1 hit
PS51026 NIFH_FRXC_3, 1 hit

Sequencei

Sequence statusi: Complete.

B4S9H5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKIAIYGKG GIGKSTTTQN TVAGLAEMGN KVMVVGCDPK ADSTRLLLGG
60 70 80 90 100
LTQKTVLDTL REEGEDVELE DIIKEGYGAS RCTESGGPEP GVGCAGRGII
110 120 130 140 150
TSVNLLEQLG AYDDEWGLDY VFYDVLGDVV CGGFAMPIRD GKAQEIYIVV
160 170 180 190 200
SGEMMAMYAA NNICKGILKY ADAGGVRLGG LICNSRKVDN EREMIEELAR
210 220 230 240 250
QLGTQMIHFV PRDNMVQRAE INRKTVIDFD PSHNQADEYR ALATKIDQNE
260 270
MFVIPKPLEI EELEKLLIDF GIAN
Length:274
Mass (Da):29,919
Last modified:September 23, 2008 - v1
Checksum:iBC48B23433CAFF78
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001108 Genomic DNA Translation: ACF46645.1
RefSeqiWP_012506178.1, NC_011059.1

Genome annotation databases

EnsemblBacteriaiACF46645; ACF46645; Paes_1627
KEGGipaa:Paes_1627

Similar proteinsi

Entry informationi

Entry nameiNIFH_PROA2
AccessioniPrimary (citable) accession number: B4S9H5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 23, 2008
Last modified: March 28, 2018
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health