B4S6B2 (B4S6B2_PROA2) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
DNA topoisomerase I EMBL ACF47214.1
EC=5.99.1.2 EMBL ACF47214.1

Gene names

Ordered Locus Names:

Paes_2212

Organism

Prosthecochloris aestuarii (strain DSM 271 / SK 413) [Complete proteome] [HAMAP] EMBL ACF47214.1

Taxonomic identifier

290512 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Prosthecochloris

Protein attributes

Sequence length	798 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. SAAS SAAS005733
Sequence similarities	Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B4S6B2 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 2E39B714B1C58EB0	FASTA	798	89,204
	10 20 30 40 50 60 MASSSAAPSA KNKTLIVVES PSKAKTINKY LGTGYKVFAS VGHIKDLPKK EIGLDFDNNY 70 80 90 100 110 120 EPRYEVIAGK EKVVRQLRKL AGEANDIMIA TDPDREGEAI AWHIANEVEG ARKPVQRVLF 130 140 150 160 170 180 NEITKNAIID AISHPRQIDY RLVRSQQTRQ GLDKIVGYKI SPFLWNVVFR GLSAGRVQSV 190 200 210 220 230 240 ALRLICERED EIGKFETREY WTLFADFTTV SGETFSAKLV KINGKDVDIT NENDATAVAD 250 260 270 280 290 300 TILSRIYGVT DISSRIQQRK PPVAFTTSLL QQAASNQLGF GSKKTMRAAQ QLYEGIELGP 310 320 330 340 350 360 EGATGLITYM RTDSTRVSAE AVGQAQSYIE QQFGPQYAGQ GRAGKNDKKT QDAHEAIRPT 370 380 390 400 410 420 SIFRTPAAMK PFLTADQFKL YELIWKRFLA SRMAPAKIEL TKVEVSDHEG EFLFRANGSK 430 440 450 460 470 480 VLFPGFLQVY SDQKELDYEA KTSTKDDEEK EQTVQLPKKL DLNEKLDLQT LDKKQSFTRP 490 500 510 520 530 540 PARYTEASLV KELDNHGIGR PSTYAAIFST LQDRRYVELL KRKIIPTELG RDVSLILVAN 550 560 570 580 590 600 FPDLFNVTFT AQMEDELDKV AAGQDDYEKV LDSFYKPLEA SLSDRKKDPV LPQNDKAERC 610 620 630 640 650 660 DKCGTGKMVV KWTSSGKFLG CSEYPKCKNI KPLSNSKPKP KGTGIACPGC EDGHMVLRDG 670 680 690 700 710 720 RFGPFLACSN YPKCNTLLNL DKQRRIQPPK TPPLETDLSC PKCGAPLYLR TGKRGLWLGC 730 740 750 760 770 780 SKFPKCRGRQ AWGQLEPALQ QHWQSVMDQH LAAHPQVTLL MTDGSPVNMQ LTVDEIMVFA 790 EEKGLIPLME EQKTGVSS

References

[1]

"Complete sequence of chromosome of Prosthecochloris aestuarii DSM 271." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Liu Z., Li T., Zhao F. , Overmann J., Bryant D.A., Richardson P. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 271 / SK 413.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001108 Genomic DNA. Translation: ACF47214.1.
RefSeq	YP_002016861.1. NC_011059.1.
3D structure databases
ProteinModelPortal	B4S6B2.
ModBase	Search...
Protein-protein interaction databases
STRING	B4S6B2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6459713.
GenomeReviews	Gene locus Paes_2212 in contig CP001108_GR.
KEGG	paa:Paes_2212.
PATRIC	23044372. VBIProAes37017_2350.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG512935.
OMA	EEYWSID.
ProtClustDB	PRK06599.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 3 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B4S6B2_PROA2

Accession

Primary (citable) accession number: B4S6B2

Entry history

Integrated into UniProtKB/TrEMBL:	September 23, 2008
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)