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B4S690 (BIOB_PROA2) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Paes_2190
OrganismProsthecochloris aestuarii (strain DSM 271 / SK 413) [Complete proteome] [HAMAP]
Taxonomic identifier290512 [NCBI]
Taxonomic lineageBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeProsthecochloris

Protein attributes

Sequence length337 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 337337Biotin synthase HAMAP-Rule MF_01694
PRO_0000381534

Sites

Metal binding731Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding771Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding801Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1521Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2131Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2831Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B4S690 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: 188F7A96FDB89149

FASTA33736,401
        10         20         30         40         50         60 
MNGAVIDPLI QEAYGVLETG RSVSYDLATR LATLRGEAVV DLLSLANKVK NRFAAPDGAL 

        70         80         90        100        110        120 
HSCSILNAKS GACSENCRFC AQSLHNQADV ESYSLLDRSK VFEAAGRVYA KGVRHFGIVT 

       130        140        150        160        170        180 
SGYGYRTVNK EFQRIIDLIT GLREEYPGLK VCASLGVLGS DSAALLADAG IAHYNINLQV 

       190        200        210        220        230        240 
SPDRYSDLIA DSHSQNDRIE TIRLLRKHRI AVCCGAILGV GETMQERIDL IFVLQALDVA 

       250        260        270        280        290        300 
VIPLNVLVPV DGTPLEGSLP LAVSDIVKTF ALCRLAHPDK VIKFAAGRET VMKDFQGLLM 

       310        320        330 
LAGANGFLTG GYLTTRGRDV GDDQAFMEQL KHFSGRS 

« Hide

References

[1]"Complete sequence of chromosome of Prosthecochloris aestuarii DSM 271."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Liu Z., Li T., Zhao F. expand/collapse author list , Overmann J., Bryant D.A., Richardson P.
Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 271 / SK 413.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001108 Genomic DNA. Translation: ACF47192.1.
RefSeqYP_002016839.1. NC_011059.1.

3D structure databases

ProteinModelPortalB4S690.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290512.Paes_2190.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACF47192; ACF47192; Paes_2190.
GeneID6459765.
KEGGpaa:Paes_2190.
PATRIC23044322. VBIProAes37017_2325.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMARDFHGMA.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycPAES290512:GHUT-2239-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_PROA2
AccessionPrimary (citable) accession number: B4S690
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 23, 2008
Last modified: May 14, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways