B4S5N0 (EFG_PROA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor G
Short name=EF-G

Gene names

Name:	fusA
Ordered Locus Names:	Paes_2067

Organism

Prosthecochloris aestuarii (strain DSM 271 / SK 413) [Complete proteome] [HAMAP]

Taxonomic identifier

290512 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Prosthecochloris

Protein attributes

Sequence length	704 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome By similarity. HAMAP MF_00054_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00054_B.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 704	704	Elongation factor G HAMAP MF_00054_B		PRO_1000091748
Regions
	Nucleotide binding	17 – 24	8	GTP By similarity
	Nucleotide binding	90 – 94	5	GTP By similarity
	Nucleotide binding	144 – 147	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4S5N0 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 1066129438B9F47E	FASTA	704	77,727
	10 20 30 40 50 60 MARQVGLDKV RNIGIMAHID AGKTTTTERI LYYTGRLHKM GEVHDGGATM DWMEQEKERG 70 80 90 100 110 120 ITITSAATTC FWEPRYGIYK GKNHRINIID TPGHVDFTVE VERSLRVLDG AVALFCAVGG 130 140 150 160 170 180 VEPQSETVWR QANKYNVPRV AYVNKMDRVG ADFFETVKSI KERLGANPVP VQIPIGQGEM 190 200 210 220 230 240 FAGVVDLIRM KGIIYDKEDG STYEEVAIPH DLESEAKHWR INMLEAVSEV DETLLEKYLE 250 260 270 280 290 300 GEDITEDEVR KVLRQATLAG TIIPTLCGSS FKNKGVQFML DTVVECLPSP VDVGNVAGHH 310 320 330 340 350 360 PDTDEDIVRE PKDEAPFAGL AFKIATDPFV GKLTFFRVYS GVLKAGSYIL NSTTGKKERI 370 380 390 400 410 420 GRLLQMHSNK REDLTEVHAG DIAAAVGMKD VKTGDTMCDE AKPIILEKMV FPEPVIQIAI 430 440 450 460 470 480 EPKTKADSDK LGMSLAKLAE EDPTFRVSSD EETGQTLIAG MGELHLEVLV DRLKREFKVE 490 500 510 520 530 540 ANVGQPQVAY RETIKKAVDH EGKFVRQSGG KGQFGLVNLK VEPLEQGKGY EFVDAIKGGV 550 560 570 580 590 600 IPKEYIPAVS NGIQEAMKDG VIAGYPVQDV KVTLYDGKYH DVDSSEMAFK IAGSIGFKGA 610 620 630 640 650 660 VRKADPVLLE PLMKVEVITP EEYLGDVMGD LSSRRGHIEG MGDRAGAQFV KAKVPLSEMF 670 680 690 700 GYSTVLRSMT QGRANYTMEF EQYHEVPKNI AEALQEKNSA KNAD

References

[1]

"Complete sequence of chromosome of Prosthecochloris aestuarii DSM 271." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Liu Z., Li T., Zhao F. , Overmann J., Bryant D.A., Richardson P. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 271 / SK 413.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001108 Genomic DNA. Translation: ACF47077.1.
RefSeq	YP_002016724.1. NC_011059.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B4S5N0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6459162.
GenomeReviews	Gene locus Paes_2067 in contig CP001108_GR.
KEGG	paa:Paes_2067.
PATRIC	23044070. VBIProAes37017_2199.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG737692.
OMA	CNEWREK.
ProtClustDB	PRK00007.
Family and domain databases
HAMAP	MF_00054_B. EF-G_EF-2_B. [Tree]
InterPro	IPR009022. Elongation_fac_G/III/V. IPR000795. ProtSyn_GTP-bd. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR005225. Small_GTP-bd_dom. IPR004540. Transl_elong_EFG/EF2. IPR000640. Transl_elong_EFG/EF2_C. IPR005517. Transl_elong_EFG/EF2_IV. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.30.70.240. Transl_elong_EFG/EF2_C. 1 hit.
KO	K02355.
Pfam	PF00679. EFG_C. 1 hit. PF03764. EFG_IV. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SMART	SM00838. EFG_C. 1 hit. SM00889. EFG_IV. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00484. EF-G. 1 hit. TIGR00231. Small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFG_PROA2

Accession

Primary (citable) accession number: B4S5N0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program