B4S5H3 (QUEF_PROA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13

Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase

Gene names

Name:	queF
Ordered Locus Names:	Paes_0514

Organism

Prosthecochloris aestuarii (strain DSM 271 / SK 413) [Complete proteome] [HAMAP]

Taxonomic identifier

290512 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Prosthecochloris

Protein attributes

Sequence length	118 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00818
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00818
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00818
Subcellular location	Cytoplasm By similarity HAMAP MF_00818.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 118	118	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00818		PRO_1000134309

Sequences

	Sequence		Length	Mass (Da)
	B4S5H3 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 238F33C8F25CCE4A	FASTA	118	13,521
	10 20 30 40 50 60 MKKELLEVFD NQFPDRDYTI EIVNPEFTSV CPKTGLPDFG TITLRYVPDK VCIELKSLKY 70 80 90 100 110 YYLEFRNAGI FYENITNTIL DHMISALHPR TLTVTTEWKA RGGITETVTA SYSSADKA

References

[1]

"Complete sequence of chromosome of Prosthecochloris aestuarii DSM 271." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Liu Z., Li T., Zhao F. , Overmann J., Bryant D.A., Richardson P. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 271 / SK 413.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001108 Genomic DNA. Translation: ACF45570.1.
RefSeq	YP_002015217.1. NC_011059.1.
3D structure databases
ProteinModelPortal	B4S5H3.
ModBase	Search...
Protein-protein interaction databases
STRING	B4S5H3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6459535.
GenomeReviews	Gene locus Paes_0514 in contig CP001108_GR.
KEGG	paa:Paes_0514.
PATRIC	23040686. VBIProAes37017_0538.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG294103.
OMA	FRNAGIF.
ProtClustDB	PRK13258.
Family and domain databases
HAMAP	MF_00818. QueF_type1. [Tree]
InterPro	IPR016856. CN_OxRdtase_NADPH-dep_QueF. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
KO	K09457.
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMs	TIGR03139. QueF-II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_PROA2

Accession

Primary (citable) accession number: B4S5H3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program