B4S404 (SYE_PROA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	Paes_1783

Organism

Prosthecochloris aestuarii (strain DSM 271 / SK 413) [Complete proteome] [HAMAP]

Taxonomic identifier

290512 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Prosthecochloris

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 503	503	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000367738
Regions
	Motif	15 – 25	11	"HIGH" region HAMAP MF_00022_B
	Motif	262 – 266	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	265	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4S404 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 943B6E8CBFBB4614	FASTA	503	57,531
	10 20 30 40 50 60 MQTTLGPRVR TRFAPSPTGY LHVGGLRTAL YNYLYAKKVG GDFIVRIEDT DQARKVEGAD 70 80 90 100 110 120 QSLLKTLEES GIVADESILH GGNFGPYMQS ERLDTYAQYC RQLLDQNNAY YCFSTAEELD 130 140 150 160 170 180 ENRKLQMKQG IQPKYNRKWL PETMGGNMPQ SEIQKKLDEG APYVIRMKVP DYISIMFEDI 190 200 210 220 230 240 VRGPVEFDSA TVDDQVLMKS DGFPTYHFAS VIDDHLMEIT HIIRGEEWLS SMPKHLLLYE 250 260 270 280 290 300 FFGWEAPRFA HLPLLLNPDR SKLSKRQGDV AVEDFIAKGY SPDAILNFVA LLGWNEGEGS 310 320 330 340 350 360 EQEIYSLEEL VQKFSLERVG KAGAVFNIDK LDWIEKQYIK ARPVDQIIQA IKPLLQKELA 370 380 390 400 410 420 EKSTDMDVAR ISSDEYLTQV IDLMRERVNF ENEFITFSNY FYFDPETYDE AAVAKRWQDD 430 440 450 460 470 480 TNEVLAEFIT ELEESDDFSA DNIEAMLKAF VAPKGKKPAF LIHPLRILSS GVGFGPSLYH 490 500 MLEVLGKQTV IRRLKKGIER VTV

References

[1]

"Complete sequence of chromosome of Prosthecochloris aestuarii DSM 271." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Liu Z., Li T., Zhao F. , Overmann J., Bryant D.A., Richardson P. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 271 / SK 413.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001108 Genomic DNA. Translation: ACF46796.1.
RefSeq	YP_002016443.1. NC_011059.1.
3D structure databases
ProteinModelPortal	B4S404.
ModBase	Search...
Protein-protein interaction databases
STRING	B4S404.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6458845.
GenomeReviews	Gene locus Paes_1783 in contig CP001108_GR.
KEGG	paa:Paes_1783.
PATRIC	23043460. VBIProAes37017_1896.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	LMEITHI.
ProtClustDB	PRK01406.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_PROA2

Accession

Primary (citable) accession number: B4S404

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program