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Protein

Thiazole synthase

Gene

thiG

Organism
Alteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei95Schiff-base intermediate with DXPUniRule annotation1
Binding sitei156DXP; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processThiamine biosynthesis
LigandSchiff base

Enzyme and pathway databases

UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotation (EC:2.8.1.10UniRule annotation)
Gene namesi
Name:thiGUniRule annotation
Ordered Locus Names:MADE_1000700
OrganismiAlteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype)
Taxonomic identifieri1774373 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeAlteromonas
Proteomesi
  • UP000001870 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001245961 – 256Thiazole synthaseAdd BLAST256

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB4S2W0
SMRiB4S2W0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni182 – 183DXP bindingUniRule annotation2
Regioni204 – 205DXP bindingUniRule annotation2

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CA8 Bacteria
COG2022 LUCA
KOiK03149
OMAiAQYPSPA

Family and domain databases

CDDicd04728 ThiG, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00443 ThiG, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR033983 Thiazole_synthase_ThiG
IPR008867 ThiG
PANTHERiPTHR34266 PTHR34266, 1 hit
PfamiView protein in Pfam
PF05690 ThiG, 1 hit

Sequencei

Sequence statusi: Complete.

B4S2W0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTLANHTFS SRLLTGTGKF SNATTMKSAV SAAQSNIVTL AMKRVASNNA
60 70 80 90 100
QDETLKALRE LGVTLLPNTS GAKNAQEAVF AAELSYEALG SQWVKLEIHP
110 120 130 140 150
DQRYLLPDPI ETLRAAETLV KKGFNVLPYC GADPVLCKRL EDVGCAAVMP
160 170 180 190 200
LGAPIGSNQG LQTAPFLKII VEQASIPVIV DAGIGKPSEA MAVMEMGVDA
210 220 230 240 250
VLVNTAIATA RNPVAMAKAF ASAVETGRCA FEAGLGATSN VALASSPLTA

FLEPTV
Length:256
Mass (Da):26,658
Last modified:September 23, 2008 - v1
Checksum:i0045148C9525D38A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001103 Genomic DNA Translation: AEA96289.1
RefSeqiWP_012516663.1, NC_011138.3

Genome annotation databases

EnsemblBacteriaiAEA96289; AEA96289; MADE_1000700
KEGGiamc:MADE_1000700
PATRICifig|314275.5.peg.145

Similar proteinsi

Entry informationi

Entry nameiTHIG_ALTMD
AccessioniPrimary (citable) accession number: B4S2W0
Secondary accession number(s): F2G2S9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 23, 2008
Last modified: March 28, 2018
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health