Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Beta-galactosidase

Gene

lacZ

Organism
Alteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na+UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei103SubstrateUniRule annotation1
Metal bindingi201SodiumUniRule annotation1
Binding sitei201SubstrateUniRule annotation1
Sitei356Transition state stabilizerUniRule annotation1
Sitei390Transition state stabilizerUniRule annotation1
Metal bindingi415Magnesium 1UniRule annotation1
Metal bindingi417Magnesium 1UniRule annotation1
Active sitei460Proton donorUniRule annotation1
Metal bindingi460Magnesium 1UniRule annotation1
Binding sitei460SubstrateUniRule annotation1
Active sitei536NucleophileUniRule annotation1
Metal bindingi596Magnesium 2UniRule annotation1
Metal bindingi600Sodium; via carbonyl oxygenUniRule annotation1
Metal bindingi603SodiumUniRule annotation1
Binding sitei603SubstrateUniRule annotation1
Binding sitei1016SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:MADE_1001350
OrganismiAlteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype)
Taxonomic identifieri1774373 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeAlteromonas
Proteomesi
  • UP000001870 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003669801 – 1041Beta-galactosidaseAdd BLAST1041

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi314275.MADE_1001350.

Structurei

3D structure databases

ProteinModelPortaliB4S2K9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni536 – 539Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CNT. Bacteria.
COG3250. LUCA.
KOiK01190.
OMAiAPIDNDM.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006103. Glyco_hydro_2_cat.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR032312. LacZ_4.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF16353. DUF4981. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4S2K9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTVAQIVAQ NDWQNPVVFQ RNRVGGHSPH HGYKTLEDAL HNANAQKYLL
60 70 80 90 100
NGEWDFCLFD APEQVPESLL AATLSAEERA KWQSIVVPSN WQLKGYDKPI
110 120 130 140 150
YCNVKYPFPV NPPIVPSENP TGCYRTTFSV TSAQLSQRNH IVFEGVNSAF
160 170 180 190 200
HLWCNGEYVG YSQDSRLPAE FDISNLLVEG ENRLAAMVIR WSDGSYLEDQ
210 220 230 240 250
DMWWLSGIFR DVSLITKPRQ HIQDVFVTPT LDACYRDATV SVRTSIKAQL
260 270 280 290 300
RCKVGIQLFD GDTTVTEQIV TGTNNKRVDE KGGWDDVVFQ TLEVKEPKHW
310 320 330 340 350
TAETPYLYRI VVSLIDDSGN VIDREAYNVG FRNVEMKNGQ LLVNGKAVLI
360 370 380 390 400
RGVNRHEHHQ VKGHAINEDD MLEDIKLLKQ NNFNAVRTAH YPNHPRWYEL
410 420 430 440 450
CDEYGLYVVD EANIETHGMF PMGRLSRDPL WAGAYMARFT QMVERDKNHP
460 470 480 490 500
SIIIWSLGNE CGHGPTHDAM YGWAKSFDPS RPVQYEGGGA DTTATDIIAP
510 520 530 540 550
MYARVDTDVE DDAVPKWAIK KWLSLPGENR PVILCEYAHA MGNSLGSFDE
560 570 580 590 600
YWKAFKDYPR LQGGFIWDWV DQGLTKHTDS GDAFWAYGGD FGDTDNDRQF
610 620 630 640 650
CINGLLFPDR TPHPHLFEAK YCQQHLSFSL TEETDKWQLS VKSDYLFRHT
660 670 680 690 700
DNELLRWQVL ENGKPIIEGE CPIYVAPQQA QTVSIAPEIN FKAGALYHLN
710 720 730 740 750
IDVVLANDCA WAKAGHVIDT AQLALANKSG LIPFVSTANV ANENAESGVT
760 770 780 790 800
VKAENTTLLV SVKNNVFSFN SESGLLTSWL HEDSEMLSAP LEDNFFRAPL
810 820 830 840 850
DNDIGVSEVD NPDPNAWESR WRRAGIGKWD RICTSVDVEQ STFDVRITSL
860 870 880 890 900
FEYHYNDKLI AATKWVYTIN HQAALTVEVE VLLDDSLPPM PRIGLQAAVP
910 920 930 940 950
APRSNEQERM RVTWQGLGPF ENYPDRKAAA RFGEHSLSIA DLQTHYIFPT
960 970 980 990 1000
DNGLRSDCKQ LNISGLRVNG QFCFSVSEYG QVQLDTAKHT SDLMPQDCVF
1010 1020 1030 1040
VYIDHAHMGV GGDDSWSPST HKAFLIEEKC YRYSVTFCAK V
Length:1,041
Mass (Da):117,714
Last modified:September 23, 2008 - v1
Checksum:i33F86621BAB56C1A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001103 Genomic DNA. Translation: AEA96419.1.
RefSeqiWP_012516793.1. NC_011138.3.

Genome annotation databases

EnsemblBacteriaiAEA96419; AEA96419; MADE_1001350.
GeneIDi6775998.
KEGGiamc:MADE_1001350.
PATRICi20871819. VBIAltMac49397_0278.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001103 Genomic DNA. Translation: AEA96419.1.
RefSeqiWP_012516793.1. NC_011138.3.

3D structure databases

ProteinModelPortaliB4S2K9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi314275.MADE_1001350.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEA96419; AEA96419; MADE_1001350.
GeneIDi6775998.
KEGGiamc:MADE_1001350.
PATRICi20871819. VBIAltMac49397_0278.

Phylogenomic databases

eggNOGiENOG4105CNT. Bacteria.
COG3250. LUCA.
KOiK01190.
OMAiAPIDNDM.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006103. Glyco_hydro_2_cat.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR032312. LacZ_4.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF16353. DUF4981. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGAL_ALTMD
AccessioniPrimary (citable) accession number: B4S2K9
Secondary accession number(s): F2G3U0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: November 2, 2016
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.