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B4S2K9 (BGAL_ALTMD) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Beta-gal
EC=3.2.1.23
Alternative name(s):
Lactase
Gene names
Name:lacZ
Ordered Locus Names:MADE_1001350
OrganismAlteromonas macleodii (strain DSM 17117 / Deep ecotype) [Complete proteome] [HAMAP]
Taxonomic identifier314275 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeAlteromonas

Protein attributes

Sequence length1041 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP-Rule MF_01687

Cofactor

Binds 2 magnesium ions per monomer By similarity. HAMAP-Rule MF_01687

Binds 1 sodium ion per monomer By similarity. HAMAP-Rule MF_01687

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_01687

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10411041Beta-galactosidase HAMAP-Rule MF_01687
PRO_0000366980

Regions

Region536 – 5394Substrate binding By similarity

Sites

Active site4601Proton donor By similarity
Active site5361Nucleophile By similarity
Metal binding2011Sodium By similarity
Metal binding4151Magnesium 1 By similarity
Metal binding4171Magnesium 1 By similarity
Metal binding4601Magnesium 1 By similarity
Metal binding5961Magnesium 2 By similarity
Metal binding6001Sodium; via carbonyl oxygen By similarity
Metal binding6031Sodium By similarity
Binding site1031Substrate By similarity
Binding site2011Substrate By similarity
Binding site4601Substrate By similarity
Binding site6031Substrate By similarity
Binding site10161Substrate By similarity
Site3561Transition state stabilizer By similarity
Site3901Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
B4S2K9 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: 33F86621BAB56C1A

FASTA1,041117,714
        10         20         30         40         50         60 
MKTVAQIVAQ NDWQNPVVFQ RNRVGGHSPH HGYKTLEDAL HNANAQKYLL NGEWDFCLFD 

        70         80         90        100        110        120 
APEQVPESLL AATLSAEERA KWQSIVVPSN WQLKGYDKPI YCNVKYPFPV NPPIVPSENP 

       130        140        150        160        170        180 
TGCYRTTFSV TSAQLSQRNH IVFEGVNSAF HLWCNGEYVG YSQDSRLPAE FDISNLLVEG 

       190        200        210        220        230        240 
ENRLAAMVIR WSDGSYLEDQ DMWWLSGIFR DVSLITKPRQ HIQDVFVTPT LDACYRDATV 

       250        260        270        280        290        300 
SVRTSIKAQL RCKVGIQLFD GDTTVTEQIV TGTNNKRVDE KGGWDDVVFQ TLEVKEPKHW 

       310        320        330        340        350        360 
TAETPYLYRI VVSLIDDSGN VIDREAYNVG FRNVEMKNGQ LLVNGKAVLI RGVNRHEHHQ 

       370        380        390        400        410        420 
VKGHAINEDD MLEDIKLLKQ NNFNAVRTAH YPNHPRWYEL CDEYGLYVVD EANIETHGMF 

       430        440        450        460        470        480 
PMGRLSRDPL WAGAYMARFT QMVERDKNHP SIIIWSLGNE CGHGPTHDAM YGWAKSFDPS 

       490        500        510        520        530        540 
RPVQYEGGGA DTTATDIIAP MYARVDTDVE DDAVPKWAIK KWLSLPGENR PVILCEYAHA 

       550        560        570        580        590        600 
MGNSLGSFDE YWKAFKDYPR LQGGFIWDWV DQGLTKHTDS GDAFWAYGGD FGDTDNDRQF 

       610        620        630        640        650        660 
CINGLLFPDR TPHPHLFEAK YCQQHLSFSL TEETDKWQLS VKSDYLFRHT DNELLRWQVL 

       670        680        690        700        710        720 
ENGKPIIEGE CPIYVAPQQA QTVSIAPEIN FKAGALYHLN IDVVLANDCA WAKAGHVIDT 

       730        740        750        760        770        780 
AQLALANKSG LIPFVSTANV ANENAESGVT VKAENTTLLV SVKNNVFSFN SESGLLTSWL 

       790        800        810        820        830        840 
HEDSEMLSAP LEDNFFRAPL DNDIGVSEVD NPDPNAWESR WRRAGIGKWD RICTSVDVEQ 

       850        860        870        880        890        900 
STFDVRITSL FEYHYNDKLI AATKWVYTIN HQAALTVEVE VLLDDSLPPM PRIGLQAAVP 

       910        920        930        940        950        960 
APRSNEQERM RVTWQGLGPF ENYPDRKAAA RFGEHSLSIA DLQTHYIFPT DNGLRSDCKQ 

       970        980        990       1000       1010       1020 
LNISGLRVNG QFCFSVSEYG QVQLDTAKHT SDLMPQDCVF VYIDHAHMGV GGDDSWSPST 

      1030       1040 
HKAFLIEEKC YRYSVTFCAK V 

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References

[1]"Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter."
Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F.
ISME J. 2:1194-1212(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 17117 / Deep ecotype.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001103 Genomic DNA. Translation: AEA96419.1.
RefSeqYP_004425417.1. NC_011138.3.

3D structure databases

ProteinModelPortalB4S2K9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING314275.MADE_00275.

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEA96419; AEA96419; MADE_1001350.
GeneID6775998.
KEGGamc:MADE_1001350.
PATRIC20871819. VBIAltMac49397_0278.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3250.
KOK01190.
OMANPPFVPK.

Enzyme and pathway databases

BioCycAMAC314275:GHA7-276-MONOMER.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_01687. Beta_gal.
InterProIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SMARTSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_ALTMD
AccessionPrimary (citable) accession number: B4S2K9
Secondary accession number(s): F2G3U0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: May 14, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries