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B4S2K9

- BGAL_ALTMD

UniProt

B4S2K9 - BGAL_ALTMD

Protein

Beta-galactosidase

Gene

lacZ

Organism
Alteromonas macleodii (strain DSM 17117 / Deep ecotype)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (23 Sep 2008)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

    Cofactori

    Binds 2 magnesium ions per monomer.UniRule annotation
    Binds 1 sodium ion per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei103 – 1031SubstrateUniRule annotation
    Metal bindingi201 – 2011SodiumUniRule annotation
    Binding sitei201 – 2011SubstrateUniRule annotation
    Sitei356 – 3561Transition state stabilizerUniRule annotation
    Sitei390 – 3901Transition state stabilizerUniRule annotation
    Metal bindingi415 – 4151Magnesium 1UniRule annotation
    Metal bindingi417 – 4171Magnesium 1UniRule annotation
    Active sitei460 – 4601Proton donorUniRule annotation
    Metal bindingi460 – 4601Magnesium 1UniRule annotation
    Binding sitei460 – 4601SubstrateUniRule annotation
    Active sitei536 – 5361NucleophileUniRule annotation
    Metal bindingi596 – 5961Magnesium 2UniRule annotation
    Metal bindingi600 – 6001Sodium; via carbonyl oxygenUniRule annotation
    Metal bindingi603 – 6031SodiumUniRule annotation
    Binding sitei603 – 6031SubstrateUniRule annotation
    Binding sitei1016 – 10161SubstrateUniRule annotation

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Sodium

    Enzyme and pathway databases

    BioCyciAMAC314275:GHA7-276-MONOMER.

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
    Short name:
    Beta-galUniRule annotation
    Alternative name(s):
    LactaseUniRule annotation
    Gene namesi
    Name:lacZUniRule annotation
    Ordered Locus Names:MADE_1001350
    OrganismiAlteromonas macleodii (strain DSM 17117 / Deep ecotype)
    Taxonomic identifieri314275 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeAlteromonas
    ProteomesiUP000001870: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10411041Beta-galactosidasePRO_0000366980Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi314275.MADE_00275.

    Structurei

    3D structure databases

    ProteinModelPortaliB4S2K9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni536 – 5394Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG3250.
    KOiK01190.
    OMAiNPPFVPK.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_01687. Beta_gal.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B4S2K9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKTVAQIVAQ NDWQNPVVFQ RNRVGGHSPH HGYKTLEDAL HNANAQKYLL     50
    NGEWDFCLFD APEQVPESLL AATLSAEERA KWQSIVVPSN WQLKGYDKPI 100
    YCNVKYPFPV NPPIVPSENP TGCYRTTFSV TSAQLSQRNH IVFEGVNSAF 150
    HLWCNGEYVG YSQDSRLPAE FDISNLLVEG ENRLAAMVIR WSDGSYLEDQ 200
    DMWWLSGIFR DVSLITKPRQ HIQDVFVTPT LDACYRDATV SVRTSIKAQL 250
    RCKVGIQLFD GDTTVTEQIV TGTNNKRVDE KGGWDDVVFQ TLEVKEPKHW 300
    TAETPYLYRI VVSLIDDSGN VIDREAYNVG FRNVEMKNGQ LLVNGKAVLI 350
    RGVNRHEHHQ VKGHAINEDD MLEDIKLLKQ NNFNAVRTAH YPNHPRWYEL 400
    CDEYGLYVVD EANIETHGMF PMGRLSRDPL WAGAYMARFT QMVERDKNHP 450
    SIIIWSLGNE CGHGPTHDAM YGWAKSFDPS RPVQYEGGGA DTTATDIIAP 500
    MYARVDTDVE DDAVPKWAIK KWLSLPGENR PVILCEYAHA MGNSLGSFDE 550
    YWKAFKDYPR LQGGFIWDWV DQGLTKHTDS GDAFWAYGGD FGDTDNDRQF 600
    CINGLLFPDR TPHPHLFEAK YCQQHLSFSL TEETDKWQLS VKSDYLFRHT 650
    DNELLRWQVL ENGKPIIEGE CPIYVAPQQA QTVSIAPEIN FKAGALYHLN 700
    IDVVLANDCA WAKAGHVIDT AQLALANKSG LIPFVSTANV ANENAESGVT 750
    VKAENTTLLV SVKNNVFSFN SESGLLTSWL HEDSEMLSAP LEDNFFRAPL 800
    DNDIGVSEVD NPDPNAWESR WRRAGIGKWD RICTSVDVEQ STFDVRITSL 850
    FEYHYNDKLI AATKWVYTIN HQAALTVEVE VLLDDSLPPM PRIGLQAAVP 900
    APRSNEQERM RVTWQGLGPF ENYPDRKAAA RFGEHSLSIA DLQTHYIFPT 950
    DNGLRSDCKQ LNISGLRVNG QFCFSVSEYG QVQLDTAKHT SDLMPQDCVF 1000
    VYIDHAHMGV GGDDSWSPST HKAFLIEEKC YRYSVTFCAK V 1041
    Length:1,041
    Mass (Da):117,714
    Last modified:September 23, 2008 - v1
    Checksum:i33F86621BAB56C1A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001103 Genomic DNA. Translation: AEA96419.1.
    RefSeqiYP_004425417.1. NC_011138.3.

    Genome annotation databases

    EnsemblBacteriaiAEA96419; AEA96419; MADE_1001350.
    GeneIDi6775998.
    KEGGiamc:MADE_1001350.
    PATRICi20871819. VBIAltMac49397_0278.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001103 Genomic DNA. Translation: AEA96419.1 .
    RefSeqi YP_004425417.1. NC_011138.3.

    3D structure databases

    ProteinModelPortali B4S2K9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 314275.MADE_00275.

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AEA96419 ; AEA96419 ; MADE_1001350 .
    GeneIDi 6775998.
    KEGGi amc:MADE_1001350.
    PATRICi 20871819. VBIAltMac49397_0278.

    Phylogenomic databases

    eggNOGi COG3250.
    KOi K01190.
    OMAi NPPFVPK.

    Enzyme and pathway databases

    BioCyci AMAC314275:GHA7-276-MONOMER.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_01687. Beta_gal.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter."
      Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F.
      ISME J. 2:1194-1212(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 17117 / Deep ecotype.

    Entry informationi

    Entry nameiBGAL_ALTMD
    AccessioniPrimary (citable) accession number: B4S2K9
    Secondary accession number(s): F2G3U0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: September 23, 2008
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3