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B4S2K9

- BGAL_ALTMD

UniProt

B4S2K9 - BGAL_ALTMD

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Protein

Beta-galactosidase

Gene
lacZ, MADE_1001350
Organism
Alteromonas macleodii (strain DSM 17117 / Deep ecotype)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Binds 2 magnesium ions per monomer By similarity.UniRule annotation
Binds 1 sodium ion per monomer By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei103 – 1031Substrate By similarity
Metal bindingi201 – 2011Sodium By similarity
Binding sitei201 – 2011Substrate By similarity
Sitei356 – 3561Transition state stabilizer By similarity
Sitei390 – 3901Transition state stabilizer By similarity
Metal bindingi415 – 4151Magnesium 1 By similarity
Metal bindingi417 – 4171Magnesium 1 By similarity
Active sitei460 – 4601Proton donor By similarity
Metal bindingi460 – 4601Magnesium 1 By similarity
Binding sitei460 – 4601Substrate By similarity
Active sitei536 – 5361Nucleophile By similarity
Metal bindingi596 – 5961Magnesium 2 By similarity
Metal bindingi600 – 6001Sodium; via carbonyl oxygen By similarity
Metal bindingi603 – 6031Sodium By similarity
Binding sitei603 – 6031Substrate By similarity
Binding sitei1016 – 10161Substrate By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Enzyme and pathway databases

BioCyciAMAC314275:GHA7-276-MONOMER.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
Ordered Locus Names:MADE_1001350
OrganismiAlteromonas macleodii (strain DSM 17117 / Deep ecotype)
Taxonomic identifieri314275 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeAlteromonas
ProteomesiUP000001870: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10411041Beta-galactosidaseUniRule annotationPRO_0000366980Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi314275.MADE_00275.

Structurei

3D structure databases

ProteinModelPortaliB4S2K9.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni536 – 5394Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3250.
KOiK01190.
OMAiNPPFVPK.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4S2K9-1 [UniParc]FASTAAdd to Basket

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MKTVAQIVAQ NDWQNPVVFQ RNRVGGHSPH HGYKTLEDAL HNANAQKYLL     50
NGEWDFCLFD APEQVPESLL AATLSAEERA KWQSIVVPSN WQLKGYDKPI 100
YCNVKYPFPV NPPIVPSENP TGCYRTTFSV TSAQLSQRNH IVFEGVNSAF 150
HLWCNGEYVG YSQDSRLPAE FDISNLLVEG ENRLAAMVIR WSDGSYLEDQ 200
DMWWLSGIFR DVSLITKPRQ HIQDVFVTPT LDACYRDATV SVRTSIKAQL 250
RCKVGIQLFD GDTTVTEQIV TGTNNKRVDE KGGWDDVVFQ TLEVKEPKHW 300
TAETPYLYRI VVSLIDDSGN VIDREAYNVG FRNVEMKNGQ LLVNGKAVLI 350
RGVNRHEHHQ VKGHAINEDD MLEDIKLLKQ NNFNAVRTAH YPNHPRWYEL 400
CDEYGLYVVD EANIETHGMF PMGRLSRDPL WAGAYMARFT QMVERDKNHP 450
SIIIWSLGNE CGHGPTHDAM YGWAKSFDPS RPVQYEGGGA DTTATDIIAP 500
MYARVDTDVE DDAVPKWAIK KWLSLPGENR PVILCEYAHA MGNSLGSFDE 550
YWKAFKDYPR LQGGFIWDWV DQGLTKHTDS GDAFWAYGGD FGDTDNDRQF 600
CINGLLFPDR TPHPHLFEAK YCQQHLSFSL TEETDKWQLS VKSDYLFRHT 650
DNELLRWQVL ENGKPIIEGE CPIYVAPQQA QTVSIAPEIN FKAGALYHLN 700
IDVVLANDCA WAKAGHVIDT AQLALANKSG LIPFVSTANV ANENAESGVT 750
VKAENTTLLV SVKNNVFSFN SESGLLTSWL HEDSEMLSAP LEDNFFRAPL 800
DNDIGVSEVD NPDPNAWESR WRRAGIGKWD RICTSVDVEQ STFDVRITSL 850
FEYHYNDKLI AATKWVYTIN HQAALTVEVE VLLDDSLPPM PRIGLQAAVP 900
APRSNEQERM RVTWQGLGPF ENYPDRKAAA RFGEHSLSIA DLQTHYIFPT 950
DNGLRSDCKQ LNISGLRVNG QFCFSVSEYG QVQLDTAKHT SDLMPQDCVF 1000
VYIDHAHMGV GGDDSWSPST HKAFLIEEKC YRYSVTFCAK V 1041
Length:1,041
Mass (Da):117,714
Last modified:September 23, 2008 - v1
Checksum:i33F86621BAB56C1A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001103 Genomic DNA. Translation: AEA96419.1.
RefSeqiYP_004425417.1. NC_011138.3.

Genome annotation databases

EnsemblBacteriaiAEA96419; AEA96419; MADE_1001350.
GeneIDi6775998.
KEGGiamc:MADE_1001350.
PATRICi20871819. VBIAltMac49397_0278.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001103 Genomic DNA. Translation: AEA96419.1 .
RefSeqi YP_004425417.1. NC_011138.3.

3D structure databases

ProteinModelPortali B4S2K9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 314275.MADE_00275.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AEA96419 ; AEA96419 ; MADE_1001350 .
GeneIDi 6775998.
KEGGi amc:MADE_1001350.
PATRICi 20871819. VBIAltMac49397_0278.

Phylogenomic databases

eggNOGi COG3250.
KOi K01190.
OMAi NPPFVPK.

Enzyme and pathway databases

BioCyci AMAC314275:GHA7-276-MONOMER.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter."
    Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F.
    ISME J. 2:1194-1212(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 17117 / Deep ecotype.

Entry informationi

Entry nameiBGAL_ALTMD
AccessioniPrimary (citable) accession number: B4S2K9
Secondary accession number(s): F2G3U0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: May 14, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi