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B4S2K9

- BGAL_ALTMD

UniProt

B4S2K9 - BGAL_ALTMD

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Protein

Beta-galactosidase

Gene

lacZ

Organism
Alteromonas macleodii (strain DSM 17117 / Deep ecotype)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na(+)UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei103 – 1031SubstrateUniRule annotation
Metal bindingi201 – 2011SodiumUniRule annotation
Binding sitei201 – 2011SubstrateUniRule annotation
Sitei356 – 3561Transition state stabilizerUniRule annotation
Sitei390 – 3901Transition state stabilizerUniRule annotation
Metal bindingi415 – 4151Magnesium 1UniRule annotation
Metal bindingi417 – 4171Magnesium 1UniRule annotation
Active sitei460 – 4601Proton donorUniRule annotation
Metal bindingi460 – 4601Magnesium 1UniRule annotation
Binding sitei460 – 4601SubstrateUniRule annotation
Active sitei536 – 5361NucleophileUniRule annotation
Metal bindingi596 – 5961Magnesium 2UniRule annotation
Metal bindingi600 – 6001Sodium; via carbonyl oxygenUniRule annotation
Metal bindingi603 – 6031SodiumUniRule annotation
Binding sitei603 – 6031SubstrateUniRule annotation
Binding sitei1016 – 10161SubstrateUniRule annotation

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Enzyme and pathway databases

BioCyciAMAC314275:GHA7-276-MONOMER.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:MADE_1001350
OrganismiAlteromonas macleodii (strain DSM 17117 / Deep ecotype)
Taxonomic identifieri314275 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeAlteromonas
ProteomesiUP000001870: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10411041Beta-galactosidasePRO_0000366980Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi314275.MADE_00275.

Structurei

3D structure databases

ProteinModelPortaliB4S2K9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni536 – 5394Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3250.
KOiK01190.
OMAiNPPFVPK.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4S2K9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKTVAQIVAQ NDWQNPVVFQ RNRVGGHSPH HGYKTLEDAL HNANAQKYLL
60 70 80 90 100
NGEWDFCLFD APEQVPESLL AATLSAEERA KWQSIVVPSN WQLKGYDKPI
110 120 130 140 150
YCNVKYPFPV NPPIVPSENP TGCYRTTFSV TSAQLSQRNH IVFEGVNSAF
160 170 180 190 200
HLWCNGEYVG YSQDSRLPAE FDISNLLVEG ENRLAAMVIR WSDGSYLEDQ
210 220 230 240 250
DMWWLSGIFR DVSLITKPRQ HIQDVFVTPT LDACYRDATV SVRTSIKAQL
260 270 280 290 300
RCKVGIQLFD GDTTVTEQIV TGTNNKRVDE KGGWDDVVFQ TLEVKEPKHW
310 320 330 340 350
TAETPYLYRI VVSLIDDSGN VIDREAYNVG FRNVEMKNGQ LLVNGKAVLI
360 370 380 390 400
RGVNRHEHHQ VKGHAINEDD MLEDIKLLKQ NNFNAVRTAH YPNHPRWYEL
410 420 430 440 450
CDEYGLYVVD EANIETHGMF PMGRLSRDPL WAGAYMARFT QMVERDKNHP
460 470 480 490 500
SIIIWSLGNE CGHGPTHDAM YGWAKSFDPS RPVQYEGGGA DTTATDIIAP
510 520 530 540 550
MYARVDTDVE DDAVPKWAIK KWLSLPGENR PVILCEYAHA MGNSLGSFDE
560 570 580 590 600
YWKAFKDYPR LQGGFIWDWV DQGLTKHTDS GDAFWAYGGD FGDTDNDRQF
610 620 630 640 650
CINGLLFPDR TPHPHLFEAK YCQQHLSFSL TEETDKWQLS VKSDYLFRHT
660 670 680 690 700
DNELLRWQVL ENGKPIIEGE CPIYVAPQQA QTVSIAPEIN FKAGALYHLN
710 720 730 740 750
IDVVLANDCA WAKAGHVIDT AQLALANKSG LIPFVSTANV ANENAESGVT
760 770 780 790 800
VKAENTTLLV SVKNNVFSFN SESGLLTSWL HEDSEMLSAP LEDNFFRAPL
810 820 830 840 850
DNDIGVSEVD NPDPNAWESR WRRAGIGKWD RICTSVDVEQ STFDVRITSL
860 870 880 890 900
FEYHYNDKLI AATKWVYTIN HQAALTVEVE VLLDDSLPPM PRIGLQAAVP
910 920 930 940 950
APRSNEQERM RVTWQGLGPF ENYPDRKAAA RFGEHSLSIA DLQTHYIFPT
960 970 980 990 1000
DNGLRSDCKQ LNISGLRVNG QFCFSVSEYG QVQLDTAKHT SDLMPQDCVF
1010 1020 1030 1040
VYIDHAHMGV GGDDSWSPST HKAFLIEEKC YRYSVTFCAK V
Length:1,041
Mass (Da):117,714
Last modified:September 23, 2008 - v1
Checksum:i33F86621BAB56C1A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001103 Genomic DNA. Translation: AEA96419.1.
RefSeqiWP_012516793.1. NC_011138.3.
YP_004425417.1. NC_011138.3.

Genome annotation databases

EnsemblBacteriaiAEA96419; AEA96419; MADE_1001350.
GeneIDi6775998.
KEGGiamc:MADE_1001350.
PATRICi20871819. VBIAltMac49397_0278.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001103 Genomic DNA. Translation: AEA96419.1 .
RefSeqi WP_012516793.1. NC_011138.3.
YP_004425417.1. NC_011138.3.

3D structure databases

ProteinModelPortali B4S2K9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 314275.MADE_00275.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AEA96419 ; AEA96419 ; MADE_1001350 .
GeneIDi 6775998.
KEGGi amc:MADE_1001350.
PATRICi 20871819. VBIAltMac49397_0278.

Phylogenomic databases

eggNOGi COG3250.
KOi K01190.
OMAi NPPFVPK.

Enzyme and pathway databases

BioCyci AMAC314275:GHA7-276-MONOMER.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter."
    Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F.
    ISME J. 2:1194-1212(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 17117 / Deep ecotype.

Entry informationi

Entry nameiBGAL_ALTMD
AccessioniPrimary (citable) accession number: B4S2K9
Secondary accession number(s): F2G3U0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: November 26, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3