Skip Header

Contribute Send feedback
Read comments (?) or add your own

B4S2H6 (GLPK_ALTMD) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycerol kinase
EC=2.7.1.30
Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK
Gene names
Name:glpK
Ordered Locus Names:MADE_1001185
OrganismAlteromonas macleodii (strain DSM 17117 / Deep ecotype) [Complete proteome] [HAMAP]
Taxonomic identifier314275 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Alteromonas

Protein attributes

Sequence length494 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Key enzyme in the regulation of glycerol uptake and metabolism By similarity. HAMAP MF_00186

Catalytic activity

ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186

Pathway

Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186

Sequence similarities

Belongs to the FGGY kinase family.

Ontologies

Keywords
   Biological processGlycerol metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processglycerol-3-phosphate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glycerol kinase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 494494Glycerol kinase HAMAP MF_00186
PRO_1000098712

Regions

Nucleotide binding409 – 4135ATP By similarity

Sites

Binding site121Substrate By similarity
Binding site161ATP By similarity
Binding site821Substrate By similarity
Binding site1351Substrate By similarity
Binding site2441Substrate By similarity
Binding site2661ATP By similarity
Binding site3091ATP; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
B4S2H6 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: 8036D4B06E7DC07E

FASTA49454,225
        10         20         30         40         50         60 
MGQHILAIDQ GTTSSRSIIF SPKRSIDAIA QQEFSQKYPK DGWVEHDPEE IWESVVSTLK 

        70         80         90        100        110        120 
EVFNKCSVAP SDIAAIGITN QRETTLVWDK HSGKPVYNAI VWQDRRTAQY CRDFSEDEAF 

       130        140        150        160        170        180 
VSYITEATGL LLDPYFSATK IAWILDNVEG AREKAENGDL LFGTVDSYLI WRLTGGESHK 

       190        200        210        220        230        240 
TDATNASRTM LFDIHNQCWD EKLLSKFNIP ASMLPEVMDC AADFGVIKEE IIGRAIPIQG 

       250        260        270        280        290        300 
VAGDQQAALV GQACFEKGMA KSTYGTGCFM ILNTGDAPLQ SKNRLLTTVG YRLNGKTTYA 

       310        320        330        340        350        360 
LEGSIFMAGA TVQWLRDGLK LIDDAAESEA LAQRAREDNG VFLVPAFTGL GAPYWDPDAR 

       370        380        390        400        410        420 
GAILGLTRDT GISEIVAAGL QSVCYQTKDL QKAMESDGAR PTTIRVDGGM SRNDWVMGFL 

       430        440        450        460        470        480 
SDILGAEVER PEITETTALG AAFLAGLQAG VFNSIDDLTH CWKSDSVFTP RLTKQERDQA 

       490 
YDGWKSAVAR IRCS

« Hide

References

[1]"Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter."
Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F.
ISME J. 2:1194-1212(2008) [PubMed: 18670397] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 17117 / Deep ecotype.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001103 Genomic DNA. Translation: AEA96386.1.
RefSeqYP_004425384.1. NC_011138.2.

3D structure databases

ProteinModelPortalB4S2H6.
SMRB4S2H6. Positions 3-492.
ModBaseSearch...

Protein-protein interaction databases

STRINGB4S2H6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6775577.
KEGGamc:MADE_1001185.
PATRIC20871753. VBIAltMac49397_0245.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG511469.
OMAMLPEVMD.

Family and domain databases

HAMAPMF_00186. Glycerol_kin.
[Tree]
InterProIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
KOK00864.
PANTHERPTHR10196. FGGY_kin. 1 hit.
PTHR10196:SF9. Glycerol_kin. 1 hit.
PfamPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01311. Glycerol_kin. 1 hit.
PROSITEPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLPK_ALTMD
AccessionPrimary (citable) accession number: B4S2H6
Secondary accession number(s): F2G3Q7
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents