B4S253 (AROE_ALTMD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Shikimate dehydrogenase
EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	MADE_1000150

Organism

Alteromonas macleodii (strain DSM 17117 / Deep ecotype) [Complete proteome] [HAMAP]

Taxonomic identifier

314275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Alteromonas

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. HAMAP MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro shikimate 3-dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	Shikimate dehydrogenase HAMAP MF_00222		PRO_1000100102
Regions
	Nucleotide binding	127 – 131	5	NADP By similarity
Sites
	Active site	66	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	B4S253 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: B27223E1458BDA98	FASTA	279	29,783
	10 20 30 40 50 60 MKKFAVFGNP IAQSLSPTIH QMFADQVGEK ISYEKILAPE DGFVEAAKAF LAQEGAVGCN 70 80 90 100 110 120 VTMPFKLDAF NLAKVDDQAA KDAQAVNTLM NGDRGELLGF NTDGVGLVND LLNSGVKIKD 130 140 150 160 170 180 KHVLLIGAGG AARGVISPLL KAGAATLTIA NRTKAKAEEV ASAASNPKVD VVALEDIATI 190 200 210 220 230 240 APHIIINSTA ASLSNALPCS LNDGVLQHCE VVYDMVYKNS PTRFMRDAAE LGVKTQIDGL 250 260 270 GMLVEQAAEA FYIWTQKRPD TSDIVKRVRD IVEQSEKRN

References

[1]

"Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter." Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F. ISME J. 2:1194-1212(2008) [PubMed: 18670397] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17117 / Deep ecotype.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001103 Genomic DNA. Translation: AEA96179.1.
RefSeq	YP_004425177.1. NC_011138.2.
3D structure databases
ProteinModelPortal	B4S253.
ModBase	Search...
Protein-protein interaction databases
STRING	B4S253.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6779063.
KEGG	amc:MADE_1000150.
PATRIC	20871315. VBIAltMac49397_0032.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG553408.
OMA	VVFGNPI.
Family and domain databases
HAMAP	MF_00222. Shikimate_DH_AroE. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR011342. Shikimate_DH. IPR013708. Shikimate_DH-bd_N. IPR022893. Shikimate_quinate_DH. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00014.
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00507. AroE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROE_ALTMD

Accession

Primary (citable) accession number: B4S253
Secondary accession number(s): F2G1T2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program