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B4S200 (PYRE_ALTMD) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Orotate phosphoribosyltransferase
Short name=OPRT
Short name=OPRTase
EC=2.4.2.10
Gene names
Name:pyrE1
Ordered Locus Names:MADE_1011205
AND
Name:pyrE2
Ordered Locus Names:MADE_1020275
OrganismAlteromonas macleodii (strain DSM 17117 / Deep ecotype) [Complete proteome] [HAMAP]
Taxonomic identifier314275 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Alteromonas

Protein attributes

Sequence length213 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208

Catalytic activity

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208

Cofactor

Magnesium By similarity. HAMAP MF_01208

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208

Subunit structure

Homodimer By similarity. HAMAP MF_01208

Sequence similarities

Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
   Biological processPyrimidine biosynthesis
   LigandMagnesium
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processnucleoside metabolic process

Inferred from electronic annotation. Source: InterPro

pyrimidine nucleotide biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionorotate phosphoribosyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 213213Orotate phosphoribosyltransferase HAMAP MF_01208
PRO_1000138759

Regions

Region34 – 352Orotate binding By similarity
Region72 – 7325-phosphoribose 1-diphosphate binding By similarity
Region124 – 13295-phosphoribose 1-diphosphate binding By similarity

Sites

Binding site2615-phosphoribose 1-diphosphate By similarity
Binding site9915-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
Binding site10015-phosphoribose 1-diphosphate By similarity
Binding site10315-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
Binding site10515-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
Binding site1281Orotate By similarity
Binding site1561Orotate By similarity

Sequences

Sequence LengthMass (Da)Tools
B4S200 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: D20D11ADE57662C7

FASTA21323,213
        10         20         30         40         50         60 
MKAFQRDFIE FAIERGVLKF GEFTLKSGRV SPYFFNAGLF NRGGDLAKLG RFYANALMDA 

        70         80         90        100        110        120 
GVEFNVLFGP AYKGIPIATT TAVALADNHN LDVPYCFNRK EAKTHGEGGN LVGSPLEGKV 

       130        140        150        160        170        180 
MLVDDVITAG TAIRESMTLI EQQQASLSGV LIALDRQERG KGELSAIQEV ERDFNTQVIS 

       190        200        210 
IVSLADVVSY LGEKGGFDNE IAAINTYREN YGI

« Hide

References

[1]"Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter."
Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F.
ISME J. 2:1194-1212(2008) [PubMed: 18670397] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 17117 / Deep ecotype.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001103 Genomic DNA. Translation: AEA98377.1.
CP001103 Genomic DNA. Translation: AEB00178.1.
RefSeqYP_004427375.1. NC_011138.2.
YP_004429175.1. NC_011138.2.

3D structure databases

ProteinModelPortalB4S200.
ModBaseSearch...

Protein-protein interaction databases

STRINGB4S200.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID10557586.
10559395.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG404341.
OMAFAFNRKE.
ProtClustDBPRK00455.

Family and domain databases

HAMAPMF_01208. PyrE.
[Tree]
InterProIPR004467. Or_phspho_trans_clade-1.
IPR023031. Orotate_PribosylTferase.
IPR000836. PRibTrfase.
[Graphical view]
PfamPF00156. Pribosyltran. 1 hit.
[Graphical view]
TIGRFAMsTIGR00336. PyrE. 1 hit.
PROSITEPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePYRE_ALTMD
AccessionPrimary (citable) accession number: B4S200
Secondary accession number(s): F2G490
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 23, 2008
Last modified: December 14, 2011
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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