Reviewed, UniProtKB/Swiss-Prot B4S1X4 (ILVC_ALTMD)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase
    Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	MADE_04021

Organism

Alteromonas macleodii (strain DSM 17117 / Deep ecotype) [Complete proteome] [HAMAP]

Taxonomic identifier

314275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Alteromonas

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 492	492	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_1000190903
Sites
	Active site	132	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	B4S1X4-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 56CF5E0475A22504	FASTA	492	54,009
	10 20 30 40 50 60 MANYFNTLSL RQQLDQLGRC RFMARDEFAD GCQFLKGKKV VIVGCGAQGL NQGLNMRDSG 70 80 90 100 110 120 LDVSYALRQA AIDEQRDSYK RASSNGFTVG TYQQLIPEAD VVYNLTPDKQ HASVVEAVMP 130 140 150 160 170 180 LMKKGATLGY SHGFNIVEEG QQIRSDITVI MCAPKSPGSE VREEYKRGFG VPTLIAVHPE 190 200 210 220 230 240 NDPEGKGWDQ AKALASATGG DRAGVLESSF VAEVKSDLMG EQTILCGMQQ VAAVLAFEKM 250 260 270 280 290 300 VDDGIDPGYA GALIQYGLEA ITEALKIGGV TNMMDRLSNP AKVKAFELSE QLTDLLRPLF 310 320 330 340 350 360 EKHQDDIISG EFSRTMMEDW ANGDANLLKW REETGETAFE NAPAYEGEIS EQEFFDHGIL 370 380 390 400 410 420 LVAMIKCGVE VAFDVMVEAG ILPESAYYES LHETPLISNT IARKRLYEMN VVISDTAEYG 430 440 450 460 470 480 NYLFANAAIP ILREKFMPTI DTTVIGKGLT AASNQVENKR LVEINEAIRS HGVESVGKTL 490 RGYMTDMKAI VV

References

[1]

"Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter." Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F. ISME J. 2:1194-1212(2008) [PubMed: 18670397] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001103 Genomic DNA. Translation: ACG68311.1.
RefSeq	YP_002128305.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6776298.
GenomeReviews	Gene locus MADE_04021 in contig CP001103_GR.
KEGG	amc:MADE_04021.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LKHGGIT.
Family and domain databases
HAMAP	MF_00435. [Tree]
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. IPR014359. KetolA_reductoisomerase_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
PIRSF	PIRSF000116. IlvC_gammaproteo. 1 hit.
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_ALTMD

Accession

Primary (citable) accession number: B4S1X4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 23, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)