B4S1X4 (ILVC_ALTMD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	MADE_1020145

Organism

Alteromonas macleodii (strain DSM 17117 / Deep ecotype) [Complete proteome] [HAMAP]

Taxonomic identifier

314275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Alteromonas

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 492	492	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_1000190903
Sites
	Active site	132	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	B4S1X4 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 56CF5E0475A22504	FASTA	492	54,009
	10 20 30 40 50 60 MANYFNTLSL RQQLDQLGRC RFMARDEFAD GCQFLKGKKV VIVGCGAQGL NQGLNMRDSG 70 80 90 100 110 120 LDVSYALRQA AIDEQRDSYK RASSNGFTVG TYQQLIPEAD VVYNLTPDKQ HASVVEAVMP 130 140 150 160 170 180 LMKKGATLGY SHGFNIVEEG QQIRSDITVI MCAPKSPGSE VREEYKRGFG VPTLIAVHPE 190 200 210 220 230 240 NDPEGKGWDQ AKALASATGG DRAGVLESSF VAEVKSDLMG EQTILCGMQQ VAAVLAFEKM 250 260 270 280 290 300 VDDGIDPGYA GALIQYGLEA ITEALKIGGV TNMMDRLSNP AKVKAFELSE QLTDLLRPLF 310 320 330 340 350 360 EKHQDDIISG EFSRTMMEDW ANGDANLLKW REETGETAFE NAPAYEGEIS EQEFFDHGIL 370 380 390 400 410 420 LVAMIKCGVE VAFDVMVEAG ILPESAYYES LHETPLISNT IARKRLYEMN VVISDTAEYG 430 440 450 460 470 480 NYLFANAAIP ILREKFMPTI DTTVIGKGLT AASNQVENKR LVEINEAIRS HGVESVGKTL 490 RGYMTDMKAI VV

References

[1]

"Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter." Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F. ISME J. 2:1194-1212(2008) [PubMed: 18670397] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17117 / Deep ecotype.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001103 Genomic DNA. Translation: AEB00152.1.
RefSeq	YP_004429149.1. NC_011138.2.
3D structure databases
ProteinModelPortal	B4S1X4.
ModBase	Search...
Protein-protein interaction databases
STRING	B4S1X4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	10559369.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG297205.
OMA	EKHMDDI.
ProtClustDB	PRK05225.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. IPR014359. KetolA_reductoisomerase_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit. PTHR21371:SF2. PTHR21371:SF2. 1 hit.
Pfam	PF01450. IlvC. 2 hits. PF07991. IlvN. 1 hit. [Graphical view]
PIRSF	PIRSF000116. IlvC_gammaproteo. 1 hit.
SUPFAM	SSF48179. 6DGDH_C_like. 2 hits.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_ALTMD

Accession

Primary (citable) accession number: B4S1X4
Secondary accession number(s): F2GCG5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 23, 2008
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program