Reviewed, UniProtKB/Swiss-Prot B4S1W8 (PLSB_ALTMD)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate acyltransferase
      Short name=GPAT
    EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	MADE_04015

Organism

Alteromonas macleodii (strain DSM 17117 / Deep ecotype) [Complete proteome] [HAMAP]

Taxonomic identifier

314275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Alteromonas

Protein attributes

Sequence length	818 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 818	818	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000123074
Regions
	Motif	308 – 313	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	B4S1W8-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 36D4704FA216914F	FASTA	818	92,322
	10 20 30 40 50 60 MSWMRKALLS VFHYPVKLLV KAHSIPVNVE TELGIDKSKP IVYLLPTNSV TDQLSLRMST 70 80 90 100 110 120 QALDLPSPTK TLTLAGREYS STLFLRKTQP LFRSSAKDTG IEEVFTDLFH LHRDHENLDL 130 140 150 160 170 180 QVVPVYVTWG RAPGRGNPGL SDLIADKAAP SWLRKLFIVL FLGRDNFINY SKAVSARAMS 190 200 210 220 230 240 NQHGSDQSIA HKLVRVASTH FQRKRQSMTG PTLLERQELN NSVLGSDAVR RAIAEESRSK 250 260 270 280 290 300 KVSHDKAKAC AQSYITEIAA DYREGLIRFG DRLLTRIWNK IYNGISVGHA ERIRELAANG 310 320 330 340 350 360 HEIIYVPCHR SHMDYLLLTY VIYHEGMVTP HIAAGINLNF WPVGKMFRRG GAFFLRRSFA 370 380 390 400 410 420 GNKLYTAVFR EYLELLFNKG YSVKYYPEGG RSRTGRLIPP KTGMLAITIQ AMLKGVNRPV 430 440 450 460 470 480 SIVPVYIGYE NVMEVKSYLN ELKGSKKKKE SNLQVFSAIR KLKNYGHGYV NFGEPIALNQ 490 500 510 520 530 540 FLESHVPNWR DCKDAEPEKK PAWLTPAVNE LANNVMTRIN RAAALNGMAL TSLCLLSSKT 550 560 570 580 590 600 QTMSEAELKQ SIGDFMDLFK AVPFSDDATI PDSTAEALLR DTLKLGRFDI KEDDYGRLIS 610 620 630 640 650 660 PQPKSAVYLT YYRNNILHLF AIPGLVMASV FAKKGTTKND ILQLIAALYP LLQKELFLHL 670 680 690 700 710 720 TQDEALAHTD ALVTALLNNG LLRQKDKELL PPDAHCKQFH SAWLLSRCMQ ETLQRYAVVL 730 740 750 760 770 780 TILDKEKVIS RGALERESKQ VAERLSALYG LSSPEFYDKN VLSSFIGALK ENHWLDSEKD 790 800 810 GSLKYSEECE ALRQDVMALI WPEMMQHLEN VALNGQTN

References

[1]

"Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter." Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F. ISME J. 2:1194-1212(2008) [PubMed: 18670397] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001103 Genomic DNA. Translation: ACG68305.1.
RefSeq	YP_002128299.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6778583.
GenomeReviews	Gene locus MADE_04015 in contig CP001103_GR.
KEGG	amc:MADE_04015.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VPVYIGY.
Family and domain databases
HAMAP	MF_00393. [Tree]
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLSB_ALTMD

Accession

Primary (citable) accession number: B4S1W8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 23, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)