Reviewed, UniProtKB/Swiss-Prot B4S1G4 (HEMH_ALTMD)

Names and origin

Protein names

Recommended name:
    Ferrochelatase
    EC=4.99.1.1
Alternative name(s):
    Protoheme ferro-lyase
    Heme synthetase

Gene names

Name:	hemH
Ordered Locus Names:	MADE_00654

Organism

Alteromonas macleodii (strain DSM 17117 / Deep ecotype) [Complete proteome] [HAMAP]

Taxonomic identifier

314275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Alteromonas

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX By similarity.
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Ferrochelatase HAMAP MF_00323		PRO_1000116029
Sites
	Metal binding	210	1	Iron By similarity
	Metal binding	291	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4S1G4-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 44B43FEABDAD83E2	FASTA	359	40,487
	10 20 30 40 50 60 MKYKSNKGFS HGQADKIGVL VTNLGTPQAP TKQALRPYLK EFLSDPRVVE VPKAIWWFVL 70 80 90 100 110 120 NGIILNFRPK RSAEAYSTVW TEEGSPLLAI TKAQAAAIGQ RCKAEYGDDV IVDFAMRYGN 130 140 150 160 170 180 PAIGKTIEDM LAKGVRKLVV LPLYPQYSAS TTGSTFDAIA KDFTSRRWLP ELRFVNHYHD 190 200 210 220 230 240 RDNFITALAN KVKAHWEAHG KADKLILSYH GIPKRYLMNG DPYHCECHKT SRLLAEKLGL 250 260 270 280 290 300 SHDEYMTTFQ SRFGREEWLT PYTDETLKSL PGKGVKSIQV MCPGFSADCL ETIEEIGEEN 310 320 330 340 350 REYFMEAGGE RYEYIAALNS DKEHIDVLFD IVQENLHGWT VESDNGLRAG LARALGAEK

References

[1]

"Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter." Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F. ISME J. 2:1194-1212(2008) [PubMed: 18670397] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001103 Genomic DNA. Translation: ACG64972.1.
RefSeq	YP_002124966.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6778047.
GenomeReviews	Gene locus MADE_00654 in contig CP001103_GR.
KEGG	amc:MADE_00654.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	MSFHGLP.
Family and domain databases
HAMAP	MF_00323. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00109. hemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_ALTMD

Accession

Primary (citable) accession number: B4S1G4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)