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B4S0P9

- BIOB_ALTMD

UniProt

B4S0P9 - BIOB_ALTMD

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Protein

Biotin synthase

Gene
bioB, MADE_1007255
Organism
Alteromonas macleodii (strain DSM 17117 / Deep ecotype)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi64 – 641Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi68 – 681Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi71 – 711Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi108 – 1081Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi139 – 1391Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi199 – 1991Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi271 – 2711Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAMAC314275:GHA7-1469-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:MADE_1007255
OrganismiAlteromonas macleodii (strain DSM 17117 / Deep ecotype)
Taxonomic identifieri314275 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeAlteromonas
ProteomesiUP000001870: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 374374Biotin synthaseUniRule annotationPRO_0000381194Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi314275.MADE_02211.

Structurei

3D structure databases

ProteinModelPortaliB4S0P9.
SMRiB4S0P9. Positions 15-326.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
KOiK01012.
OMAiRIMMPAS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B4S0P9-1 [UniParc]FASTAAdd to Basket

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MTLAYAPTAQ TITIRNDWTK AEVEALFAMP FNDLLFNAQV VHRQHFNPNE    50
VQVSTLLSIK TGACPEDCKY CPQSARYDTG LEKERLLEIE KVIQRAKEAK 100
QVGSTRFCMG AAWRNPRDRD MPYILKMVEE VKSLGLETCM TLGMLTRDQA 150
VALKQAGLDY YNHNLDTSPE YYGDIITTRT YEDRLNTLEN VRAAGMNVCS 200
GGIVGMGETV SDRASMLVQL ANLPEQPQSV PINMLVKVKG TPLDSVEDLD 250
YFEFIRTIAV ARIMMPKSHV RLSAGREAMN EQMQAMCFMA GANSIFYGCK 300
LLTTSNPDTH EDVMLFKKLG INTERTRDYS DEAHQQVLEE EIAQQQEQAE 350
GSNDLFIDAT KPKVAAKQQH ATEA 374
Length:374
Mass (Da):41,999
Last modified:September 23, 2008 - v1
Checksum:i949F05B2DF1C24D2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001103 Genomic DNA. Translation: AEA97593.1.
RefSeqiWP_012517935.1. NC_011138.3.
YP_004426591.1. NC_011138.3.

Genome annotation databases

EnsemblBacteriaiAEA97593; AEA97593; MADE_1007255.
GeneIDi10556793.
KEGGiamc:MADE_1007255.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001103 Genomic DNA. Translation: AEA97593.1 .
RefSeqi WP_012517935.1. NC_011138.3.
YP_004426591.1. NC_011138.3.

3D structure databases

ProteinModelPortali B4S0P9.
SMRi B4S0P9. Positions 15-326.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 314275.MADE_02211.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AEA97593 ; AEA97593 ; MADE_1007255 .
GeneIDi 10556793.
KEGGi amc:MADE_1007255.

Phylogenomic databases

eggNOGi COG0502.
KOi K01012.
OMAi RIMMPAS.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci AMAC314275:GHA7-1469-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter."
    Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F.
    ISME J. 2:1194-1212(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 17117 / Deep ecotype.

Entry informationi

Entry nameiBIOB_ALTMD
AccessioniPrimary (citable) accession number: B4S0P9
Secondary accession number(s): F2G6Q6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 23, 2008
Last modified: September 3, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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