Reviewed, UniProtKB/Swiss-Prot B4S0F6 (AROK_ALTMD)

Names and origin

Protein names

Recommended name:
    Shikimate kinase
      Short name=SK
    EC=2.7.1.71

Gene names

Name:	aroK
Ordered Locus Names:	MADE_00510

Organism

Alteromonas macleodii (strain DSM 17117 / Deep ecotype) [Complete proteome] [HAMAP]

Taxonomic identifier

314275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Alteromonas

Protein attributes

Sequence length	171 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate By similarity.
Catalytic activity	ATP + shikimate = ADP + shikimate 3-phosphate. HAMAP MF_00109
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7. HAMAP MF_00109
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the shikimate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP shikimate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 171	171	Shikimate kinase HAMAP MF_00109		PRO_1000094371
Regions
	Nucleotide binding	14 – 19	6	ATP By similarity
Sites
	Metal binding	18	1	Magnesium By similarity
	Binding site	36	1	Substrate By similarity
	Binding site	60	1	Substrate By similarity
	Binding site	82	1	Substrate; via amide nitrogen By similarity
	Binding site	120	1	ATP By similarity
	Binding site	139	1	Substrate By similarity
	Binding site	156	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4S0F6-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 27FB0442E02F833F	FASTA	171	19,181
	10 20 30 40 50 60 MAEKRNIFLV GPMGAGKSTI GRHLADELHL DFFDSDQEIE RRTGADIAWI FDLEGEDGFR 70 80 90 100 110 120 AREENVINDL TDKQGIVLAT GGGSIVTKAV RNRLSARGIV VYLQTTIDKQ VARTQRDKRR 130 140 150 160 170 PLLQNEDPEQ VLRDLAEMRN PLYEEVADYI VDTDDQSARA VANQIISKIG L

References

[1]

"Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter." Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F. ISME J. 2:1194-1212(2008) [PubMed: 18670397] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001103 Genomic DNA. Translation: ACG64828.1.
RefSeq	YP_002124822.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6777425.
GenomeReviews	Gene locus MADE_00510 in contig CP001103_GR.
KEGG	amc:MADE_00510.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	HANVFFV.
Family and domain databases
HAMAP	MF_00109. [Tree]
InterPro	IPR000623. Shik_kinase. [Graphical view]
Pfam	PF01202. SKI. 1 hit. [Graphical view]
PRINTS	PR01100. SHIKIMTKNASE.
PROSITE	PS01128. SHIKIMATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROK_ALTMD

Accession

Primary (citable) accession number: B4S0F6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)