Reviewed, UniProtKB/Swiss-Prot B4RZX7 (PUR5_ALTMD)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine cyclo-ligase
    EC=6.3.3.1
Alternative name(s):
    AIRS
    Phosphoribosyl-aminoimidazole synthetase
    AIR synthase

Gene names

Name:	purM
Ordered Locus Names:	MADE_02148

Organism

Alteromonas macleodii (strain DSM 17117 / Deep ecotype) [Complete proteome] [HAMAP]

Taxonomic identifier

314275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Alteromonas

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP MF_00741
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP MF_00741
Subcellular location	Cytoplasm By similarity HAMAP MF_00741.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP MF_00741		PRO_1000192989

Sequences

	Sequence		Length	Mass (Da)
	B4RZX7-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: C74E2D1D9BAC0547	FASTA	346	37,011
	10 20 30 40 50 60 MSENKTSLSY KDAGVDIDAG NQLVERIKSV TKKTHRPEVK GNLGGFGALC ELPTKYKKPL 70 80 90 100 110 120 LVSGTDGVGT KLRVAMDANR HDGVGIDLVA MCVNDLIVQG AEPLFFLDYY ATGKLDVDVA 130 140 150 160 170 180 ASVVTGIGAG CEQAGCALIG GETAEMPGMY HDGDYDIAGF CVGVVEADNV IDGTNVKPGQ 190 200 210 220 230 240 KLIALGSSGP HSNGYSLVRK IIEVSGADVN AELNGKPIID QLLEPTRIYV KSVLALLEEV 250 260 270 280 290 300 QVSAISHITG GGFWENIPRV LPEDAKVVID EKSWEWPAVF SWLQENGNVT RHEMYRTFNC 310 320 330 340 GVGLVIVVDD ADTEQAVNIL KQHGENAWVI GDIASKDGEE QVEINA

References

[1]

"Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter." Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F. ISME J. 2:1194-1212(2008) [PubMed: 18670397] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001103 Genomic DNA. Translation: ACG66448.1.
RefSeq	YP_002126442.1.
3D structure databases
SMR	B4RZX7. Positions 6-344.
ModBase	Search...
Genome annotation databases
GeneID	6775714.
GenomeReviews	Gene locus MADE_02148 in contig CP001103_GR.
KEGG	amc:MADE_02148.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG531222.
OMA	HYDLAGF.
Family and domain databases
HAMAP	MF_00741_B. AIRS_B. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00878. purM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_ALTMD

Accession

Primary (citable) accession number: B4RZX7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 23, 2008
Last modified:	February 9, 2010
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)