B4RZB4 (RLMG_ALTMD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase G
EC=2.1.1.174

Alternative name(s):
23S rRNA m2G1835 methyltransferase
rRNA (guanine-N(2)-)-methyltransferase RlmG

Gene names

Name:	rlmG
Ordered Locus Names:	MADE_1003650

Organism

Alteromonas macleodii (strain DSM 17117 / Deep ecotype) [Complete proteome] [HAMAP]

Taxonomic identifier

314275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Alteromonas

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP MF_01859
Catalytic activity	S-adenosyl-L-methionine + guanosine(1835) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanosine(1835) in 23S rRNA. HAMAP MF_01859
Subcellular location	Cytoplasm Potential HAMAP MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 410	410	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366448

Sequences

	Sequence		Length	Mass (Da)
	B4RZB4 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: ED14E8EE11353804	FASTA	410	45,313
	10 20 30 40 50 60 MNTEFIFADR QLSLIRYPEK HQHVSLQAWD SADELVIEHL ESLLSENELS IGDNESTPSL 70 80 90 100 110 120 MIFNDDFGAL GCWFSHLAPY WVSDSYISLR SLHENLKANS LIRASEGSCT QELKTSPVKT 130 140 150 160 170 180 LTSVESASFK PACAPAVVVI KVPRALALLE QQLIDLQAYI TPETQVIATG KVKAITKSAL 190 200 210 220 230 240 NLFEKYIGPT TTSLAKKKSR LIFATPRDSL KQATSPYPTR WQCKSTMGAA LIIDNLANTF 250 260 270 280 290 300 ARQSLDIGAR IMLEHMTVSA NDVVVDLGCG NGVLGVNALS LAPDAKVIFV DESYMALESA 310 320 330 340 350 360 RLNVLNNFPD KIEQCEFVAS NCLETLLNRE NKPAVTKILC NPPFHQQNAI TDHIAWQMFT 370 380 390 400 410 DSRDLLVKSG HLVVVGNRHL EYHIKLKKLF GGAKVLASDK KFVILGTAKR

References

[1]

"Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter." Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F. ISME J. 2:1194-1212(2008) [PubMed: 18670397] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17117 / Deep ecotype.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001103 Genomic DNA. Translation: AEA96878.1.
RefSeq	YP_004425876.1. NC_011138.2.
3D structure databases
ProteinModelPortal	B4RZB4.
ModBase	Search...
Protein-protein interaction databases
STRING	B4RZB4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	10556075.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG321931.
OMA	NRHLGYH.
ProtClustDB	CLSK2315384.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G. [Tree]
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_ALTMD

Accession

Primary (citable) accession number: B4RZB4
Secondary accession number(s): F2G966

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	December 14, 2011
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program