Reviewed, UniProtKB/Swiss-Prot B4RYS6 (CYSI_ALTMD)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] hemoprotein beta-component
      Short name=SiR-HP
      Short name=SiRHP
    EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	MADE_03588

Organism

Alteromonas macleodii (strain DSM 17117 / Deep ecotype) [Complete proteome] [HAMAP]

Taxonomic identifier

314275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Alteromonas

Protein attributes

Sequence length	573 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity. HAMAP MF_01540
Catalytic activity	H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH. HAMAP MF_01540
Cofactor	Binds 1 siroheme per subunit By similarity. HAMAP MF_01540 Binds 1 4Fe-4S cluster per subunit By similarity. HAMAP MF_01540
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1. HAMAP MF_01540
Subunit structure	Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity. HAMAP MF_01540
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW hydrogen sulfide biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADP or NADPH binding Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 573	573	Sulfite reductase [NADPH] hemoprotein beta-component HAMAP MF_01540		PRO_1000146642
Sites
	Metal binding	436	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	442	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	481	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	485	1	Iron (siroheme axial ligand) By similarity
	Metal binding	485	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4RYS6-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: E2A6169D732D1744	FASTA	573	63,913
	10 20 30 40 50 60 MSNEKSPFIV EGKLADNERL KRESNNLRGT ITTDLKDDLT GGFTADNFQL IRFHGMYQQD 70 80 90 100 110 120 DRDIRAERAK QKLEPLHNVM LRARLPGGVI TPDQWLAIDK FADDHTMYGS IRLTTRQTFQ 130 140 150 160 170 180 FHGVLKPQIK LMHQTLNKVG IDSIATAGDV NRNVLCTSNP VESELHLHAY EWAKKISEHL 190 200 210 220 230 240 LPKTRAYAEI WLDGEKVETT EKPVEPILGD NYLPRKFKTT VVIPPQNDVD VHANDLNFVA 250 260 270 280 290 300 IAENGQLVGF NVLVGGGLAM THGDKSTYPR KASDFGFIPL ENTLDVAAAV VTTQRDWGNR 310 320 330 340 350 360 VNRKNAKTKY TLERVGVDNF KAEVEKRAGV TFQESRPYEF TDRGDRFGWV EGIDGKYHLT 370 380 390 400 410 420 VFIENGRILD YPGKTLKTGC AEIAKIHKGD FRLTANQNLI IAGVPPQDKD KIEALAREHG 430 440 450 460 470 480 LIAPSISNQR LDSMACVALP TCPLAMAEAE RYLPDAVTEL EGLLAKHGLE EDSVIFRVTG 490 500 510 520 530 540 CPNGCGRAML SEVGLVGKGP GKYNLHLGGN REGTRIPRMY RENISEQEIM AELDTLLGQW 550 560 570 AKERDSGEAF GDYVVRAGVV KPVVDSARDF YDQ

References

[1]

"Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter." Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F. ISME J. 2:1194-1212(2008) [PubMed: 18670397] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001103 Genomic DNA. Translation: ACG67880.1.
RefSeq	YP_002127874.1.
3D structure databases
SMR	B4RYS6. Positions 80-572.
ModBase	Search...
Genome annotation databases
GeneID	6779105.
GenomeReviews	Gene locus MADE_03588 in contig CP001103_GR.
KEGG	amc:MADE_03588.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG626671.
OMA	EEIVIRM.
Family and domain databases
HAMAP	MF_01540. CysI. [Tree]
InterPro	IPR011786. CysI. IPR005117. NiRdtase/SiRdtase_haem-b_fer. IPR006067. NO2/SO3_Rdtase_4Fe4S_dom. IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS. [Graphical view]
Pfam	PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSI_ALTMD

Accession

Primary (citable) accession number: B4RYS6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 23, 2008
Last modified:	February 9, 2010
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)