Reviewed, UniProtKB/Swiss-Prot B4RYN9 (PANC_ALTMD)

Names and origin

Protein names

Recommended name:
    Pantothenate synthetase
      Short name=PS
    EC=6.3.2.1
Alternative name(s):
    Pantoate--beta-alanine ligase
    Pantoate-activating enzyme

Gene names

Name:	panC
Ordered Locus Names:	MADE_03551

Organism

Alteromonas macleodii (strain DSM 17117 / Deep ecotype) [Complete proteome] [HAMAP]

Taxonomic identifier

314275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Alteromonas

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate By similarity. HAMAP MF_00158
Catalytic activity	ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate. HAMAP MF_00158
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1. HAMAP MF_00158
Subunit structure	Homodimer By similarity. HAMAP MF_00158
Subcellular location	Cytoplasm Potential HAMAP MF_00158.
Miscellaneous	The reaction proceeds by a bi uni uni bi ping pong mechanism By similarity. HAMAP MF_00158
Sequence similarities	Belongs to the pantothenate synthetase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pantoate-beta-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	Pantothenate synthetase HAMAP MF_00158		PRO_1000097028
Regions
	Nucleotide binding	30 – 37	8	ATP By similarity
	Nucleotide binding	149 – 152	4	ATP By similarity
	Nucleotide binding	186 – 189	4	ATP By similarity
Sites
	Active site	37	1	Proton donor By similarity
	Binding site	61	1	Beta-alanine By similarity
	Binding site	61	1	Pantoate By similarity
	Binding site	155	1	Pantoate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4RYN9-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: AC943C3604B879E8	FASTA	282	30,833
	10 20 30 40 50 60 MKVIDSISTL RQTVNAWRRN GESVGFVPTM GNLHDGHLKL VKKAKAHNDN VVVSIFVNPM 70 80 90 100 110 120 QFGANEDLDA YPRTIEEDKA KLISVGADAV FLPSVAEMYP AGLDAQTFVE VPGISDSHCG 130 140 150 160 170 180 ASRPGHFRGV ATVVTKLFNM VQPDDAFFGE KDFQQLQVIR ALARDLSMAV TIHGVPTERE 190 200 210 220 230 240 ASGLAMSSRN GYLSKEEKAT ASAIYEEMQR VKAGIEGGNI DFSELEDAMV TNLEAKGFKK 250 260 270 280 DYCQVVNAST FKAATANDKE LVLLVAMFMG KTRLIDNLQI TR

References

[1]

"Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter." Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F. ISME J. 2:1194-1212(2008) [PubMed: 18670397] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001103 Genomic DNA. Translation: ACG67843.1.
RefSeq	YP_002127837.1.
3D structure databases
SMR	B4RYN9. Positions 1-281.
ModBase	Search...
Genome annotation databases
GeneID	6777961.
GenomeReviews	Gene locus MADE_03551 in contig CP001103_GR.
KEGG	amc:MADE_03551.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG428839.
OMA	MPIQIIG.
Family and domain databases
HAMAP	MF_00158. PanC. [Tree]
InterPro	IPR004821. Cyt_trans_rel. IPR003721. Pantoate_ligase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR21299:SF1. Pantoate_ligase. 1 hit.
Pfam	PF02569. Pantoate_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00125. cyt_tran_rel. 1 hit. TIGR00018. panC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANC_ALTMD

Accession

Primary (citable) accession number: B4RYN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	February 9, 2010
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)