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B4RYH7 (SYQ_ALTMD) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine--tRNA ligase

EC=6.1.1.18
Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS
Gene names
Name:glnS
Ordered Locus Names:MADE_1009580
OrganismAlteromonas macleodii (strain DSM 17117 / Deep ecotype) [Complete proteome] [HAMAP]
Taxonomic identifier314275 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeAlteromonas

Protein attributes

Sequence length555 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP-Rule MF_00126

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00126

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00126.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutaminyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

glutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 555555Glutamine--tRNA ligase HAMAP-Rule MF_00126
PRO_1000095477

Regions

Motif34 – 4411"HIGH" region HAMAP-Rule MF_00126
Motif268 – 2725"KMSKS" region HAMAP-Rule MF_00126

Sites

Binding site2711ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B4RYH7 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: ECF1E7277A6FA339

FASTA55563,478
        10         20         30         40         50         60 
MAETEHRPTN FIRQIIDKDL SNGLHDAIKT RFPPEPNGFL HIGHAKSICL NFGIAEDYTG 

        70         80         90        100        110        120 
SCNLRFDDTN PAKEDITFVN SIKEDVTWLG FTWDGEARYS SNYFDQLHAY AVELIEKGLA 

       130        140        150        160        170        180 
YVDFSAQDVM REMRGTLKEP GQNSPYRDTD VETNLKEFAK MTAGDYKEGE CSLRAKIDMT 

       190        200        210        220        230        240 
SPFMCMRDPV IYRVKHAHHH QTGDKWCVYP MYDFTHCISD AIEGITHSLC TLEFQDNRRL 

       250        260        270        280        290        300 
YDWVIENISI DCTPRQYEFS RLNLEYTVLS KRRLIQLVEE NHVSGWDDPR MPTIAGLRRR 

       310        320        330        340        350        360 
GYTPGSIVEF CKRIGVTKMD NMVEMSMLEA CIRDDLNANA PRAMAVLDPI KLVIENYEEG 

       370        380        390        400        410        420 
KTETLVAPNH PNDESMGTRN IGFSREVYIE AEDFRESANK KFKRLVLEKE VRLRNAYVVK 

       430        440        450        460        470        480 
ANRVEKDEEG NVTTVYCTYD PDTLGKDPAD GRKVKGVIHW VDAATAQAAE FRLYDRLFNV 

       490        500        510        520        530        540 
PNPAAEENFT DAINPESLEV KQGWAEAGLI GHVPEVGAWQ FERTGYFCVD KDSTDAKPVF 

       550 
NRTVGLRDTW AKIGE 

« Hide

References

[1]"Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter."
Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F.
ISME J. 2:1194-1212(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 17117 / Deep ecotype.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001103 Genomic DNA. Translation: AEA98054.1.
RefSeqYP_004427052.1. NC_011138.3.

3D structure databases

ProteinModelPortalB4RYH7.
SMRB4RYH7. Positions 9-549.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING314275.MADE_01892.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEA98054; AEA98054; MADE_1009580.
GeneID10557258.
KEGGamc:MADE_1009580.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
KOK01886.
OMAVTHSICT.
ProtClustDBPRK05347.

Enzyme and pathway databases

BioCycAMAC314275:GHA7-1936-MONOMER.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPMF_00126. Gln_tRNA_synth.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00440. glnS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYQ_ALTMD
AccessionPrimary (citable) accession number: B4RYH7
Secondary accession number(s): F2GC38
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: February 19, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries