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B4RXT8 (IF2_ALTMD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Translation initiation factor IF-2
Gene names
Name:infB
Ordered Locus Names:MADE_1008920
OrganismAlteromonas macleodii (strain DSM 17117 / Deep ecotype) [Complete proteome] [HAMAP]
Taxonomic identifier314275 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeAlteromonas

Protein attributes

Sequence length868 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex By similarity. HAMAP-Rule MF_00100

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00100.

Sequence similarities

Belongs to the IF-2 family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandGTP-binding
Nucleotide-binding
   Molecular functionInitiation factor
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

translation initiation factor activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 868868Translation initiation factor IF-2 HAMAP-Rule MF_00100
PRO_1000093755

Regions

Nucleotide binding377 – 3848GTP By similarity
Nucleotide binding423 – 4275GTP By similarity
Nucleotide binding477 – 4804GTP By similarity
Region371 – 519149G-domain HAMAP-Rule MF_00100

Sequences

Sequence LengthMass (Da)Tools
B4RXT8 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: 32AA374822B8D3FD

FASTA86895,580
        10         20         30         40         50         60 
MADVSIEKLA SDIGTTVDRL VGQFKDAGIS KSAGEQVNED EKQKLLDHLS KQHGSAAEPT 

        70         80         90        100        110        120 
RMTLKRKTTS TLSVGKSKEV KVEVRKKRTY VKRSDIEEQQ RQAEEEAKRL EEEARLKREA 

       130        140        150        160        170        180 
EEKAAAEAKK AAEEKARKAQ EAKKAAEEER VRRAEQAKKE AEARKKDEPE LTEAEKAEAE 

       190        200        210        220        230        240 
AARQEEERLR KAQEEEAQKK LEEDAKKAAD EARKLAEENE RRWKEEEERR KKAEAEEVHL 

       250        260        270        280        290        300 
HSNRYAQEAE DEEDMQVERS SRRRRKSKKN AGEHLKQGFN KPAAPVERVV KLGATITVGE 

       310        320        330        340        350        360 
LASKLAIKSN EVIKTMMKMG EMATINQVLD QDTAVLVIEE MGHKYELVND NALEDELLAD 

       370        380        390        400        410        420 
GTDGEKTSRA PVVTIMGHVD HGKTSLLDYI RRAKVADGEA GGITQHIGAY KVQTDNGEIT 

       430        440        450        460        470        480 
FLDTPGHAAF TAMRARGATA TDIVILVVAA DDGVMPQTKE AVQHARAAGV PLIVAVNKMD 

       490        500        510        520        530        540 
KETADPDRVK TELSQLEVIS EEWGGEHQFC NVSAKTGMGV DELLEAIVLQ SELLDLQAVA 

       550        560        570        580        590        600 
EGPGRGIVIE SRLDKGRGPV ASVLVQEGQL RAGDILLCGE EYGRVRAMRD ENGKDMKLAG 

       610        620        630        640        650        660 
PSTPVEVLGL SGVPVAGEDA AVVKDERKAR EVAAKRHQKK RELKLARQQK AKLENMFANM 

       670        680        690        700        710        720 
ESGDVSELNI VLKADVQGSV EAISESLIKL STSEVKVNIV GSGVGGITET DATLAAASGA 

       730        740        750        760        770        780 
IVLGFNVRAD ATARRVLEAE EIDLRYYSVI YNLIDEVKAA MSGMLAPEFK QEIIGLAEVR 

       790        800        810        820        830        840 
DVFKSPKLGA IAGCMVTEGN VKRSNPIRVL RDNVVIYEGE LESLRRFKDD VQDVRNGMEC 

       850        860 
GIGVKNYNDV KVGDQIEVFE IVEVKREI 

« Hide

References

[1]"Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter."
Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F.
ISME J. 2:1194-1212(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 17117 / Deep ecotype.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001103 Genomic DNA. Translation: AEA97922.1.
RefSeqYP_004426920.1. NC_011138.3.

3D structure databases

ProteinModelPortalB4RXT8.
SMRB4RXT8. Positions 2-50.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING314275.MADE_01760.

Proteomic databases

PRIDEB4RXT8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEA97922; AEA97922; MADE_1008920.
GeneID10557126.
KEGGamc:MADE_1008920.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0532.
KOK02519.
OMAMMKLGQM.
ProtClustDBPRK05306.

Enzyme and pathway databases

BioCycAMAC314275:GHA7-1804-MONOMER.

Family and domain databases

Gene3D3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00100_B. IF_2_B.
InterProIPR009061. DNA-bd_dom_put.
IPR000795. EF_GTP-bd_dom.
IPR013575. IF2_assoc_dom_bac.
IPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR015760. TIF_IF2.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PANTHERPTHR23115:SF41. PTHR23115:SF41. 1 hit.
PfamPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF11987. IF-2. 1 hit.
PF08364. IF2_assoc. 1 hit.
PF04760. IF2_N. 2 hits.
[Graphical view]
SUPFAMSSF46955. SSF46955. 1 hit.
SSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS01176. IF2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIF2_ALTMD
AccessionPrimary (citable) accession number: B4RXT8
Secondary accession number(s): F2GAC8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: April 16, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Translation initiation factors

List of translation initiation factor entries