Reviewed, UniProtKB/Swiss-Prot B4RW95 (HUTI_ALTMD)

Names and origin

Protein names

Recommended name:
    Imidazolonepropionase
    EC=3.5.2.7
Alternative name(s):
    Imidazolone-5-propionate hydrolase

Gene names

Name:	hutI
Ordered Locus Names:	MADE_03129

Organism

Alteromonas macleodii (strain DSM 17117 / Deep ecotype) [Complete proteome] [HAMAP]

Taxonomic identifier

314275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Alteromonas

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372
Cofactor	Binds 1 zinc or iron ion per subunit By similarity.
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the hutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazolonepropionase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	Imidazolonepropionase HAMAP MF_00372		PRO_1000121527
Sites
	Metal binding	87	1	Zinc or iron By similarity
	Metal binding	89	1	Zinc or iron By similarity
	Metal binding	257	1	Zinc or iron By similarity
	Metal binding	332	1	Zinc or iron By similarity
	Binding site	96	1	Substrate By similarity
	Binding site	109	1	Substrate By similarity
	Binding site	159	1	Substrate By similarity
	Binding site	192	1	Substrate By similarity
	Binding site	260	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4RW95-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 250D5FB21BD60771	FASTA	419	45,544
	10 20 30 40 50 60 MDSKYDTLIY NVRIASMQHN GMPYGELPPT AIAIKEGHIV ALLSCDEQLN DALQQTSTVI 70 80 90 100 110 120 DGSRLIPCNE NEQPTLPWLL PGFIDCHTHL VYGGNRAEEF EMRLQGASYV EIAERGGGIK 130 140 150 160 170 180 GTVKKTRQAD EQTLLNTAIK RAMRLCEEGV TTIEVKSGYG LDVDTEIRML SVAKSLEKHL 190 200 210 220 230 240 PVTIQTTYLG AHALPNEFNH DADAYIDFIC RDALPQIASL GLADAVDVFC ETIGFSLQQT 250 260 270 280 290 300 ERVFSCAKQY GLPVKAHVEQ LSDSKGAVLA AKYGALSVDH IEYLVDSDIP SLVKSETVAV 310 320 330 340 350 360 LLPGAFYYLS EVQKPPVEAL RAHNVPIAVA TDFNPGSSPL ASLLTALNMS CVLFKLTPEE 370 380 390 400 410 ALRGATEHAA SALGLNDRGV VDIGNVADLT LWDIETPAEL VYCINGHRPI AVFKDGKHV

References

[1]

"Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter." Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F. ISME J. 2:1194-1212(2008) [PubMed: 18670397] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001103 Genomic DNA. Translation: ACG67423.1.
RefSeq	YP_002127417.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6777455.
GenomeReviews	Gene locus MADE_03129 in contig CP001103_GR.
KEGG	amc:MADE_03129.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	MNMACTL.
Family and domain databases
HAMAP	MF_00372. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR005920. HutI. [Graphical view]
Pfam	PF01979. Amidohydro_1. 2 hits. [Graphical view]
TIGRFAMs	TIGR01224. hutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUTI_ALTMD

Accession

Primary (citable) accession number: B4RW95

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 23, 2008
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)