B4RW95 (HUTI_ALTMD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Imidazolonepropionase
EC=3.5.2.7

Alternative name(s):
Imidazolone-5-propionate hydrolase

Gene names

Name:	hutI
Ordered Locus Names:	MADE_1004495

Organism

Alteromonas macleodii (strain DSM 17117 / Deep ecotype) [Complete proteome] [HAMAP]

Taxonomic identifier

314275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Alteromonas

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372
Cofactor	Binds 1 zinc or iron ion per subunit By similarity. HAMAP MF_00372
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372
Subcellular location	Cytoplasm Potential HAMAP MF_00372.
Sequence similarities	Belongs to the HutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazolonepropionase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	Imidazolonepropionase HAMAP MF_00372		PRO_1000121527
Sites
	Metal binding	87	1	Zinc or iron By similarity
	Metal binding	89	1	Zinc or iron By similarity
	Metal binding	257	1	Zinc or iron By similarity
	Metal binding	332	1	Zinc or iron By similarity
	Binding site	96	1	Substrate By similarity
	Binding site	109	1	Substrate By similarity
	Binding site	159	1	Substrate By similarity
	Binding site	192	1	Substrate By similarity
	Binding site	260	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4RW95 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 250D5FB21BD60771	FASTA	419	45,544
	10 20 30 40 50 60 MDSKYDTLIY NVRIASMQHN GMPYGELPPT AIAIKEGHIV ALLSCDEQLN DALQQTSTVI 70 80 90 100 110 120 DGSRLIPCNE NEQPTLPWLL PGFIDCHTHL VYGGNRAEEF EMRLQGASYV EIAERGGGIK 130 140 150 160 170 180 GTVKKTRQAD EQTLLNTAIK RAMRLCEEGV TTIEVKSGYG LDVDTEIRML SVAKSLEKHL 190 200 210 220 230 240 PVTIQTTYLG AHALPNEFNH DADAYIDFIC RDALPQIASL GLADAVDVFC ETIGFSLQQT 250 260 270 280 290 300 ERVFSCAKQY GLPVKAHVEQ LSDSKGAVLA AKYGALSVDH IEYLVDSDIP SLVKSETVAV 310 320 330 340 350 360 LLPGAFYYLS EVQKPPVEAL RAHNVPIAVA TDFNPGSSPL ASLLTALNMS CVLFKLTPEE 370 380 390 400 410 ALRGATEHAA SALGLNDRGV VDIGNVADLT LWDIETPAEL VYCINGHRPI AVFKDGKHV

References

[1]

"Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter." Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F. ISME J. 2:1194-1212(2008) [PubMed: 18670397] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17117 / Deep ecotype.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001103 Genomic DNA. Translation: AEA97045.1.
RefSeq	YP_004426043.1. NC_011138.2.
3D structure databases
ProteinModelPortal	B4RW95.
ModBase	Search...
Protein-protein interaction databases
STRING	B4RW95.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	10556240.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG686142.
OMA	MNMACTL.
ProtClustDB	PRK09356.
Family and domain databases
HAMAP	MF_00372. HutI. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR005920. HutI. IPR011059. Metal-dep_hydrolase_composite. [Graphical view]
PANTHER	PTHR22642. PTHR22642. 1 hit.
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
SUPFAM	SSF51338. Metalo_hydrolase. 1 hit.
TIGRFAMs	TIGR01224. HutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUTI_ALTMD

Accession

Primary (citable) accession number: B4RW95
Secondary accession number(s): F2GB04

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program